(Linear) (Six-base) MAPSORT of: KIEE0673.seq  Check: 7252  from: 1  to: 783



KIEE0673.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AccI GT'mk_AC

Cuts at:      0     110     481     691     783 
   Size:        110     371     210      92

  Fragments arranged by size: 

                 371     210     110      92
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     783 
   Size:        212     571
 
ApoI r'AATT_y

Cuts at:      0     516     783 
   Size:        516     267
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     648     783 
   Size:        648     135
 
BbvCI CC'TCA_GC

Cuts at:      0     486     783 
   Size:        486     297
 
BclI T'GATC_A

Cuts at:      0     321     783 
   Size:        321     462
 
BglII A'GATC_T

Cuts at:      0     751     783 
   Size:        751      32
 
Bpu10I CC'TnA_GC

Cuts at:      0     486     615     783 
   Size:        486     129     168

  Fragments arranged by size: 

                 486     168     129
 
BsaHI Gr'CG_yC

Cuts at:      0      56     783 
   Size:         56     727
 
BseYI C'CCAG_C

Cuts at:      0     343     783 
   Size:        343     440
 
BsmI GAATG_Cn'

Cuts at:      0     726     783 
   Size:        726      57
 
BssSI C'ACGA_G

Cuts at:      0      12     783 
   Size:         12     771
 
BstYI r'GATC_y

Cuts at:      0     664     751     783 
   Size:        664      87      32

  Fragments arranged by size: 

                 664      87      32
 
BstZ17I GTA'TAC

Cuts at:      0     692     783 
   Size:        692      91
 
Bsu36I CC'TnA_GG

Cuts at:      0      68     783 
   Size:         68     715
 
DraIII CAC_nnn'GTG

Cuts at:      0     262     783 
   Size:        262     521
 
EaeI y'GGCC_r

Cuts at:      0     231     783 
   Size:        231     552
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     783 
   Size:        212     571
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     489     783 
   Size:        489     294
 
EcoO109I rG'GnC_Cy

Cuts at:      0      17     783 
   Size:         17     766
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     217     783 
   Size:        217     566
 
PsiI TTA'TAA

Cuts at:      0     206     783 
   Size:        206     577
 
SfcI C'TryA_G

Cuts at:      0     104     783 
   Size:        104     679
 
StuI AGG'CCT

Cuts at:      0      66     783 
   Size:         66     717
 
StyI C'CwwG_G

Cuts at:      0     472     783 
   Size:        472     311
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     728     783 
   Size:        728      55
 
TatI w'GTAC_w

Cuts at:      0     711     783 
   Size:        711      72
 
XbaI T'CTAG_A

Cuts at:      0     526     783 
   Size:        526     257
 
XmnI GAAnn'nnTTC

Cuts at:      0     179     372     645     783 
   Size:        179     193     273     138

  Fragments arranged by size: 

                 273     193     179     138
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     BbsI    BbvCI     BclI    BglII   Bpu10I
    BsaHI    BseYI     BsmI    BssSI    BstYI  BstZ17I   Bsu36I   DraIII
     EaeI  Eco57MI    EcoNI EcoO109I     FalI     PsiI     SfcI     StuI
     StyI    TaqII     TatI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII   BstAPI    BstBI   BstEII    BstXI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EagI     EarI     EciI  EcoICRI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TstI  Tth111I     XcmI     XhoI     XmaI     ZraI