(Linear) (Six-base) MAPSORT of: KIEE0674.seq  Check: 9923  from: 1  to: 1188



KIEE0674.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AccI GT'mk_AC

Cuts at:      0    1074    1188 
   Size:       1074     114
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     177     329     447     867    1188 
   Size:        177     152     118     420     321

  Fragments arranged by size: 

                 420     321     177     152     118
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     598    1188 
   Size:        598     590
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     239     576    1188 
   Size:        239     337     612

  Fragments arranged by size: 

                 612     337     239
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1067    1188 
   Size:       1067     121
 
Bme1580I G_kGCm'C

Cuts at:      0     398    1188 
   Size:        398     790
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     851     935    1055    1188 
   Size:        851      84     120     133

  Fragments arranged by size: 

                 851     133     120      84
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     695     779    1188 
   Size:        695      84     409

  Fragments arranged by size: 

                 695     409      84
 
BsaAI yAC'GTr

Cuts at:      0    1055    1188 
   Size:       1055     133
 
BsaWI w'CCGG_w

Cuts at:      0     505    1188 
   Size:        505     683
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67    1188 
   Size:         67    1121
 
BsmI GAATG_Cn'

Cuts at:      0     563     796     964    1048    1170    1188 
   Size:        563     233     168      84     122      18

  Fragments arranged by size: 

                 563     233     168     122      84      18
 
Bsp1286I G_dGCh'C

Cuts at:      0     398    1188 
   Size:        398     790
 
BsrDI GCAATG_nn'

Cuts at:      0     790    1188 
   Size:        790     398
 
BstBI TT'CG_AA

Cuts at:      0     567    1188 
   Size:        567     621
 
BstYI r'GATC_y

Cuts at:      0     979    1188 
   Size:        979     209
 
BtsI GCAGTG_nn'

Cuts at:      0    1126    1188 
   Size:       1126      62
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     177     329     447     695     779     867    1188 
   Size:        177     152     118     248      84      88     321

  Fragments arranged by size: 

                 321     248     177     152     118      88      84
 
EcoO109I rG'GnC_Cy

Cuts at:      0       9    1085    1188 
   Size:          9    1076     103

  Fragments arranged by size: 

                1076     103       9
 
EcoRV GAT'ATC

Cuts at:      0     469    1188 
   Size:        469     719
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     477    1188 
   Size:        477     711
 
HincII GTy'rAC

Cuts at:      0     445    1188 
   Size:        445     743
 
HindIII A'AGCT_T

Cuts at:      0     299    1188 
   Size:        299     889
 
MslI CAynn'nnrTG

Cuts at:      0     438     580     858    1188 
   Size:        438     142     278     330

  Fragments arranged by size: 

                 438     330     278     142
 
NdeI CA'TA_TG

Cuts at:      0     855    1188 
   Size:        855     333
 
PfoI T'CCnGG_A

Cuts at:      0     287    1188 
   Size:        287     901
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     112     144    1188 
   Size:        112      32    1044

  Fragments arranged by size: 

                1044     112      32
 
PpuMI rG'GwC_Cy

Cuts at:      0       9    1085    1188 
   Size:          9    1076     103

  Fragments arranged by size: 

                1076     103       9
 
PsiI TTA'TAA

Cuts at:      0     605    1142    1188 
   Size:        605     537      46

  Fragments arranged by size: 

                 605     537      46
 
SfcI C'TryA_G

Cuts at:      0      90     131    1188 
   Size:         90      41    1057

  Fragments arranged by size: 

                1057      90      41
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      61    1188 
   Size:         61    1127
 
XmnI GAAnn'nnTTC

Cuts at:      0     460     796    1188 
   Size:        460     336     392

  Fragments arranged by size: 

                 460     392     336
 

 Enzymes that do cut:

     AccI     AcuI     AlfI     BbsI    BciVI Bme1580I     BmrI     BpmI
    BsaAI    BsaWI    BseRI     BsmI Bsp1286I    BsrDI    BstBI    BstYI
     BtsI  Eco57MI EcoO109I    EcoRV     FalI   HincII  HindIII     MslI
     NdeI     PfoI     PpiI    PpuMI     PsiI     SfcI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRI     FseI     FspI    FspAI    HaeII    Hin4I     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI