(Linear) (Six-base) MAPSORT of: KIEE0676.seq  Check: 689  from: 1  to: 1188



KIEE0676.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AccI GT'mk_AC

Cuts at:      0     351    1188 
   Size:        351     837
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     334     397    1188 
   Size:        158     176      63     791

  Fragments arranged by size: 

                 791     176     158      63
 
AflII C'TTAA_G

Cuts at:      0     474    1188 
   Size:        474     714
 
AleI CACnn'nnGTG

Cuts at:      0     701    1188 
   Size:        701     487
 
ApoI r'AATT_y

Cuts at:      0     168     284     479     676     954    1188 
   Size:        168     116     195     197     278     234

  Fragments arranged by size: 

                 278     234     197     195     168     116
 
BanII G_rGCy'C

Cuts at:      0      40    1188 
   Size:         40    1148
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      36     650    1188 
   Size:         36     614     538

  Fragments arranged by size: 

                 614     538      36
 
BglI GCCn_nnn'nGGC

Cuts at:      0     137    1188 
   Size:        137    1051
 
BlpI GC'TnA_GC

Cuts at:      0     469    1188 
   Size:        469     719
 
BmgBI CAC'GTC

Cuts at:      0     721    1188 
   Size:        721     467
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     215    1188 
   Size:        215     973
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       5    1188 
   Size:          5    1183
 
BsaAI yAC'GTr

Cuts at:      0     961    1188 
   Size:        961     227
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1156    1188 
   Size:       1156      32
 
BsiHKAI G_wGCw'C

Cuts at:      0      40    1188 
   Size:         40    1148
 
Bsp1286I G_dGCh'C

Cuts at:      0      40    1188 
   Size:         40    1148
 
BssSI C'ACGA_G

Cuts at:      0       5     505     681    1188 
   Size:          5     500     176     507

  Fragments arranged by size: 

                 507     500     176       5
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     906    1188 
   Size:        906     282
 
BstYI r'GATC_y

Cuts at:      0     798    1188 
   Size:        798     390
 
BtsI GCAGTG_nn'

Cuts at:      0     195    1188 
   Size:        195     993
 
ClaI AT'CG_AT

Cuts at:      0     689    1188 
   Size:        689     499
 
DraIII CAC_nnn'GTG

Cuts at:      0     202    1188 
   Size:        202     986
 
EaeI y'GGCC_r

Cuts at:      0     129     768    1188 
   Size:        129     639     420

  Fragments arranged by size: 

                 639     420     129
 
EcoICRI GAG'CTC

Cuts at:      0      38    1188 
   Size:         38    1150
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     334     397    1188 
   Size:        158     176      63     791

  Fragments arranged by size: 

                 791     176     158      63
 
EcoRI G'AATT_C

Cuts at:      0     168     479     676    1188 
   Size:        168     311     197     512

  Fragments arranged by size: 

                 512     311     197     168
 
EcoRV GAT'ATC

Cuts at:      0     531    1188 
   Size:        531     657
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     378     410    1188 
   Size:        378      32     778

  Fragments arranged by size: 

                 778     378      32
 
HindIII A'AGCT_T

Cuts at:      0     471    1188 
   Size:        471     717
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      54    1188 
   Size:         54    1134
 
MscI TGG'CCA

Cuts at:      0     131     770    1188 
   Size:        131     639     418

  Fragments arranged by size: 

                 639     418     131
 
MslI CAynn'nnrTG

Cuts at:      0     509     515     701    1188 
   Size:        509       6     186     487

  Fragments arranged by size: 

                 509     487     186       6
 
SacI G_AGCT'C

Cuts at:      0      40    1188 
   Size:         40    1148
 
SexAI A'CCwGG_T

Cuts at:      0     572     885    1101    1188 
   Size:        572     313     216      87

  Fragments arranged by size: 

                 572     313     216      87
 
SfcI C'TryA_G

Cuts at:      0     989    1188 
   Size:        989     199
 
SmlI C'TyrA_G

Cuts at:      0      20     474    1188 
   Size:         20     454     714

  Fragments arranged by size: 

                 714     454      20
 
SspI AAT'ATT

Cuts at:      0     843    1188 
   Size:        843     345
 
StuI AGG'CCT

Cuts at:      0     538    1188 
   Size:        538     650
 
StyI C'CwwG_G

Cuts at:      0     132     778    1188 
   Size:        132     646     410

  Fragments arranged by size: 

                 646     410     132
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     313    1188 
   Size:        313     875
 
TatI w'GTAC_w

Cuts at:      0     578    1188 
   Size:        578     610
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     139     763    1188 
   Size:        139     624     425

  Fragments arranged by size: 

                 624     425     139
 
XmnI GAAnn'nnTTC

Cuts at:      0     500     623    1188 
   Size:        500     123     565

  Fragments arranged by size: 

                 565     500     123
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AleI     ApoI    BanII     BbsI     BglI
     BlpI    BmgBI     BmrI    BpuEI    BsaAI    BseRI  BsiHKAI Bsp1286I
    BssSI    BstXI    BstYI     BtsI     ClaI   DraIII     EaeI  EcoICRI
  Eco57MI    EcoRI    EcoRV    Hin4I  HindIII     MmeI     MscI     MslI
     SacI    SexAI     SfcI     SmlI     SspI     StuI     StyI    TaqII
     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII Bme1580I     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     BtgI    BtgZI    CspCI     DraI     DrdI     EagI     EarI
     EciI    EcoNI EcoO109I     FalI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI
     ZraI