Restriction map for
pF1KE0682
(Linear) (Six-base) MAPSORT of: KIEE0682.seq Check: 1916 from: 1 to: 1167
KIEE0682.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 0 776 1167
Size: 776 391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 1108 1167
Size: 45 1063 59
Fragments arranged by size:
1063 59 45
AlwNI CAG_nnn'CTG
Cuts at: 0 98 108 277 495 1167
Size: 98 10 169 218 672
Fragments arranged by size:
672 218 169 98 10
AvaI C'yCGr_G
Cuts at: 0 221 1167
Size: 221 946
BanI G'GyrC_C
Cuts at: 0 183 446 953 1167
Size: 183 263 507 214
Fragments arranged by size:
507 263 214 183
BanII G_rGCy'C
Cuts at: 0 332 554 716 1167
Size: 332 222 162 451
Fragments arranged by size:
451 332 222 162
BbeI G_GCGC'C
Cuts at: 0 450 1167
Size: 450 717
BbsI GAAGACnn'nnnn_
Cuts at: 0 244 1167
Size: 244 923
BbvCI CC'TCA_GC
Cuts at: 0 758 1167
Size: 758 409
BglI GCCn_nnn'nGGC
Cuts at: 0 142 1167
Size: 142 1025
BlpI GC'TnA_GC
Cuts at: 0 523 1167
Size: 523 644
Bme1580I G_kGCm'C
Cuts at: 0 956 1167
Size: 956 211
BmrI ACTGGGnnnn_n'
Cuts at: 0 636 670 1167
Size: 636 34 497
Fragments arranged by size:
636 497 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 165 1167
Size: 165 1002
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 346 1104 1167
Size: 209 137 758 63
Fragments arranged by size:
758 209 137 63
Bpu10I CC'TnA_GC
Cuts at: 0 436 572 727 758 1167
Size: 436 136 155 31 409
Fragments arranged by size:
436 409 155 136 31
BsaI GGTCTCn'nnnn_
Cuts at: 0 211 1167
Size: 211 956
BsaAI yAC'GTr
Cuts at: 0 453 1167
Size: 453 714
BsaHI Gr'CG_yC
Cuts at: 0 447 1167
Size: 447 720
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1079 1109 1167
Size: 1079 30 58
Fragments arranged by size:
1079 58 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 1151 1167
Size: 193 958 16
Fragments arranged by size:
958 193 16
BseYI C'CCAG_C
Cuts at: 0 431 459 719 901 1016 1167
Size: 431 28 260 182 115 151
Fragments arranged by size:
431 260 182 151 115 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 1167
Size: 487 680
BsmI GAATG_Cn'
Cuts at: 0 168 1023 1167
Size: 168 855 144
Fragments arranged by size:
855 168 144
BsmBI CGTCTCn'nnnn_
Cuts at: 0 532 1167
Size: 532 635
Bsp1286I G_dGCh'C
Cuts at: 0 332 554 716 956 1167
Size: 332 222 162 240 211
Fragments arranged by size:
332 240 222 211 162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 503 1167
Size: 503 664
BsrBI CCG'CTC
Cuts at: 0 522 1167
Size: 522 645
BstAPI GCAn_nnn'nTGC
Cuts at: 0 98 277 495 1167
Size: 98 179 218 672
Fragments arranged by size:
672 218 179 98
BstEII G'GTnAC_C
Cuts at: 0 375 1167
Size: 375 792
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1082 1167
Size: 1082 85
Bsu36I CC'TnA_GG
Cuts at: 0 338 592 1167
Size: 338 254 575
Fragments arranged by size:
575 338 254
BtgI C'CryG_G
Cuts at: 0 288 872 1167
Size: 288 584 295
Fragments arranged by size:
584 295 288
BtsI GCAGTG_nn'
Cuts at: 0 116 1167
Size: 116 1051
DraIII CAC_nnn'GTG
Cuts at: 0 456 1167
Size: 456 711
EarI CTCTTCn'nnn_
Cuts at: 0 964 1167
Size: 964 203
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 209 346 1104 1108 1167
Size: 45 164 137 758 4 59
Fragments arranged by size:
758 164 137 59 45 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 191 989 1167
Size: 191 798 178
Fragments arranged by size:
798 191 178
EcoO109I rG'GnC_Cy
Cuts at: 0 135 492 630 933 1051 1167
Size: 135 357 138 303 118 116
Fragments arranged by size:
357 303 138 135 118 116
HaeII r_GCGC'y
Cuts at: 0 450 476 1167
Size: 450 26 691
Fragments arranged by size:
691 450 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1079 1111 1167
Size: 1079 32 56
Fragments arranged by size:
1079 56 32
KasI G'GCGC_C
Cuts at: 0 446 1167
Size: 446 721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 1167
Size: 176 991
MslI CAynn'nnrTG
Cuts at: 0 82 913 1167
Size: 82 831 254
Fragments arranged by size:
831 254 82
MspA1I CmG'CkG
Cuts at: 0 723 762 1020 1167
Size: 723 39 258 147
Fragments arranged by size:
723 258 147 39
NarI GG'CG_CC
Cuts at: 0 447 1167
Size: 447 720
NcoI C'CATG_G
Cuts at: 0 288 872 1167
Size: 288 584 295
Fragments arranged by size:
584 295 288
PasI CC'CwG_GG
Cuts at: 0 889 1167
Size: 889 278
PflMI CCAn_nnn'nTGG
Cuts at: 0 108 1167
Size: 108 1059
PmlI CAC'GTG
Cuts at: 0 453 1167
Size: 453 714
PpuMI rG'GwC_Cy
Cuts at: 0 492 630 1167
Size: 492 138 537
Fragments arranged by size:
537 492 138
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 97 129 1167
Size: 97 32 1038
Fragments arranged by size:
1038 97 32
PstI C_TGCA'G
Cuts at: 0 212 263 491 582 1167
Size: 212 51 228 91 585
Fragments arranged by size:
585 228 212 91 51
PvuII CAG'CTG
Cuts at: 0 723 762 1020 1167
Size: 723 39 258 147
Fragments arranged by size:
723 258 147 39
SapI GCTCTTCn'nnn_
Cuts at: 0 964 1167
Size: 964 203
SbfI CC_TGCA'GG
Cuts at: 0 212 582 1167
Size: 212 370 585
Fragments arranged by size:
585 370 212
SfcI C'TryA_G
Cuts at: 0 208 259 487 578 1167
Size: 208 51 228 91 589
Fragments arranged by size:
589 228 208 91 51
SfoI GGC'GCC
Cuts at: 0 448 1167
Size: 448 719
SmaI CCC'GGG
Cuts at: 0 223 1167
Size: 223 944
StuI AGG'CCT
Cuts at: 0 984 1167
Size: 984 183
StyI C'CwwG_G
Cuts at: 0 30 288 872 948 1152 1167
Size: 30 258 584 76 204 15
Fragments arranged by size:
584 258 204 76 30 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 613 1167
Size: 613 554
TatI w'GTAC_w
Cuts at: 0 644 1167
Size: 644 523
XbaI T'CTAG_A
Cuts at: 0 1111 1167
Size: 1111 56
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1006 1167
Size: 1006 161
XmaI C'CCGG_G
Cuts at: 0 221 1167
Size: 221 946
Enzymes that do cut:
AccI AcuI AlwNI AvaI BanI BanII BbeI BbsI
BbvCI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsmI
BsmBI Bsp1286I BspMI BsrBI BstAPI BstEII BstXI Bsu36I
BtgI BtsI DraIII EarI Eco57MI EcoNI EcoO109I HaeII
Hin4I KasI MmeI MslI MspA1I NarI NcoI PasI
PflMI PmlI PpuMI PsrI PstI PvuII SapI SbfI
SfcI SfoI SmaI StuI StyI TaqII TatI XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglII BmgBI BmtI BpuEI BsaBI BsaWI BsiEI
BsiHKAI BsiWI BspEI BspHI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstYI BstZ17I BtgZI ClaI CspCI DraI
DrdI EaeI EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MscI NaeI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PpiI PshAI
PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI
SanDI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XhoI
XmnI ZraI