Restriction map for
pF1KE0687
(Linear) (Six-base) MAPSORT of: KIEE0687.seq Check: 9043 from: 1 to: 1284
KIEE0687.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 972 1284
Size: 972 312
AleI CACnn'nnGTG
Cuts at: 0 370 1284
Size: 370 914
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 562 1284
Size: 562 722
ApoI r'AATT_y
Cuts at: 0 721 1284
Size: 721 563
AseI AT'TA_AT
Cuts at: 0 691 1284
Size: 691 593
AvaI C'yCGr_G
Cuts at: 0 58 446 828 1284
Size: 58 388 382 456
Fragments arranged by size:
456 388 382 58
BanI G'GyrC_C
Cuts at: 0 364 497 546 1272 1284
Size: 364 133 49 726 12
Fragments arranged by size:
726 364 133 49 12
BanII G_rGCy'C
Cuts at: 0 65 845 1284
Size: 65 780 439
Fragments arranged by size:
780 439 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 927 961 1284
Size: 927 34 323
Fragments arranged by size:
927 323 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1006 1284
Size: 1006 278
BlpI GC'TnA_GC
Cuts at: 0 160 1284
Size: 160 1124
Bme1580I G_kGCm'C
Cuts at: 0 367 1284
Size: 367 917
BmtI G_CTAG'C
Cuts at: 0 734 1284
Size: 734 550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 1284
Size: 859 425
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1284
Size: 295 989
BsaBI GATnn'nnATC
Cuts at: 0 982 1284
Size: 982 302
BsaWI w'CCGG_w
Cuts at: 0 887 1284
Size: 887 397
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1006 1036 1069 1099 1284
Size: 1006 30 33 30 185
Fragments arranged by size:
1006 185 33 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 35 229 1057 1074 1219 1222 1284
Size: 35 194 828 17 145 3 62
Fragments arranged by size:
828 194 145 62 35 17 3
BseYI C'CCAG_C
Cuts at: 0 168 1284
Size: 168 1116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 1284
Size: 148 1136
BsiHKAI G_wGCw'C
Cuts at: 0 845 1284
Size: 845 439
BsmBI CGTCTCn'nnnn_
Cuts at: 0 67 1284
Size: 67 1217
Bsp1286I G_dGCh'C
Cuts at: 0 65 367 845 1284
Size: 65 302 478 439
Fragments arranged by size:
478 439 302 65
BspEI T'CCGG_A
Cuts at: 0 887 1284
Size: 887 397
BspHI T'CATG_A
Cuts at: 0 515 1115 1284
Size: 515 600 169
Fragments arranged by size:
600 515 169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 818 1284
Size: 818 466
BsrFI r'CCGG_y
Cuts at: 0 859 1284
Size: 859 425
BstEII G'GTnAC_C
Cuts at: 0 222 1284
Size: 222 1062
BstXI CCAn_nnnn'nTGG
Cuts at: 0 915 1284
Size: 915 369
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 812 1284
Size: 812 472
EaeI y'GGCC_r
Cuts at: 0 917 1145 1284
Size: 917 228 139
Fragments arranged by size:
917 228 139
EarI CTCTTCn'nnn_
Cuts at: 0 280 967 1284
Size: 280 687 317
Fragments arranged by size:
687 317 280
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1120 1284
Size: 1120 164
EcoICRI GAG'CTC
Cuts at: 0 843 1284
Size: 843 441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 972 1284
Size: 859 113 312
Fragments arranged by size:
859 312 113
EcoO109I rG'GnC_Cy
Cuts at: 0 824 1284
Size: 824 460
HaeII r_GCGC'y
Cuts at: 0 335 995 1035 1284
Size: 335 660 40 249
Fragments arranged by size:
660 335 249 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1006 1038 1284
Size: 1006 32 246
Fragments arranged by size:
1006 246 32
HincII GTy'rAC
Cuts at: 0 12 1095 1284
Size: 12 1083 189
Fragments arranged by size:
1083 189 12
MscI TGG'CCA
Cuts at: 0 919 1147 1284
Size: 919 228 137
Fragments arranged by size:
919 228 137
MslI CAynn'nnrTG
Cuts at: 0 190 370 1262 1284
Size: 190 180 892 22
Fragments arranged by size:
892 190 180 22
MspA1I CmG'CkG
Cuts at: 0 124 779 852 1284
Size: 124 655 73 432
Fragments arranged by size:
655 432 124 73
NdeI CA'TA_TG
Cuts at: 0 684 1259 1284
Size: 684 575 25
Fragments arranged by size:
684 575 25
NheI G'CTAG_C
Cuts at: 0 730 1284
Size: 730 554
NspI r_CATG'y
Cuts at: 0 285 942 1284
Size: 285 657 342
Fragments arranged by size:
657 342 285
PasI CC'CwG_GG
Cuts at: 0 1180 1284
Size: 1180 104
PflMI CCAn_nnn'nTGG
Cuts at: 0 113 1001 1284
Size: 113 888 283
Fragments arranged by size:
888 283 113
PfoI T'CCnGG_A
Cuts at: 0 660 1284
Size: 660 624
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 47 79 1284
Size: 47 32 1205
Fragments arranged by size:
1205 47 32
PvuII CAG'CTG
Cuts at: 0 852 1284
Size: 852 432
SacI G_AGCT'C
Cuts at: 0 845 1284
Size: 845 439
SexAI A'CCwGG_T
Cuts at: 0 493 1284
Size: 493 791
SmaI CCC'GGG
Cuts at: 0 448 1284
Size: 448 836
SmlI C'TyrA_G
Cuts at: 0 310 1284
Size: 310 974
SphI G_CATG'C
Cuts at: 0 285 942 1284
Size: 285 657 342
Fragments arranged by size:
657 342 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 791 1284
Size: 791 493
TatI w'GTAC_w
Cuts at: 0 140 1284
Size: 140 1144
XbaI T'CTAG_A
Cuts at: 0 408 1284
Size: 408 876
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 357 1284
Size: 357 927
XmaI C'CCGG_G
Cuts at: 0 446 1284
Size: 446 838
Enzymes that do cut:
AcuI AleI AlfI ApoI AseI AvaI BanI BanII
BcgI BglI BlpI Bme1580I BmtI BpmI BpuEI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrFI BstEII BstXI BtgZI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII
MscI MslI MspA1I NdeI NheI NspI PasI PflMI
PfoI PpiI PvuII SacI SexAI SmaI SmlI SphI
TaqII TatI XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BmgBI BmrI BplI Bpu10I BsaI BsaAI
BsaHI BsiEI BsiWI BsmI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtsI
ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NgoMIV
NotI NruI NsiI PacI PciI PmeI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
StyI SwaI TstI Tth111I XhoI XmnI ZraI