Restriction map for
pF1KE0690
(Linear) (Six-base) MAPSORT of: KIEE0690.seq Check: 8088 from: 1 to: 1203
KIEE0690.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 893 1203
Size: 703 190 310
Fragments arranged by size:
703 310 190
AflIII A'CryG_T
Cuts at: 0 1192 1203
Size: 1192 11
ApaI G_GGCC'C
Cuts at: 0 279 351 1203
Size: 279 72 852
Fragments arranged by size:
852 279 72
ApaLI G'TGCA_C
Cuts at: 0 670 1203
Size: 670 533
ApoI r'AATT_y
Cuts at: 0 340 1203
Size: 340 863
AvaI C'yCGr_G
Cuts at: 0 110 141 278 313 779 1203
Size: 110 31 137 35 466 424
Fragments arranged by size:
466 424 137 110 35 31
BamHI G'GATC_C
Cuts at: 0 120 500 1203
Size: 120 380 703
Fragments arranged by size:
703 380 120
BanI G'GyrC_C
Cuts at: 0 19 75 368 718 774 1203
Size: 19 56 293 350 56 429
Fragments arranged by size:
429 350 293 56 56 19
BanII G_rGCy'C
Cuts at: 0 279 351 454 524 986 1203
Size: 279 72 103 70 462 217
Fragments arranged by size:
462 279 217 103 72 70
BbeI G_GCGC'C
Cuts at: 0 23 79 372 1203
Size: 23 56 293 831
Fragments arranged by size:
831 293 56 23
BbvCI CC'TCA_GC
Cuts at: 0 91 515 840 1203
Size: 91 424 325 363
Fragments arranged by size:
424 363 325 91
BglI GCCn_nnn'nGGC
Cuts at: 0 157 283 1203
Size: 157 126 920
Fragments arranged by size:
920 157 126
Bme1580I G_kGCm'C
Cuts at: 0 279 351 674 1203
Size: 279 72 323 529
Fragments arranged by size:
529 323 279 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 58 1203
Size: 58 1145
Bpu10I CC'TnA_GC
Cuts at: 0 91 515 840 1203
Size: 91 424 325 363
Fragments arranged by size:
424 363 325 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 1203
Size: 346 857
BsaI GGTCTCn'nnnn_
Cuts at: 0 472 557 964 1203
Size: 472 85 407 239
Fragments arranged by size:
472 407 239 85
BsaHI Gr'CG_yC
Cuts at: 0 20 76 308 369 1203
Size: 20 56 232 61 834
Fragments arranged by size:
834 232 61 56 20
BsaWI w'CCGG_w
Cuts at: 0 123 1203
Size: 123 1080
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 409 532 1203
Size: 409 123 671
Fragments arranged by size:
671 409 123
BseYI C'CCAG_C
Cuts at: 0 79 425 600 766 1203
Size: 79 346 175 166 437
Fragments arranged by size:
437 346 175 166 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 637 1203
Size: 406 231 566
Fragments arranged by size:
566 406 231
BsiEI CG_ry'CG
Cuts at: 0 134 199 1203
Size: 134 65 1004
Fragments arranged by size:
1004 134 65
BsiHKAI G_wGCw'C
Cuts at: 0 454 674 986 1203
Size: 454 220 312 217
Fragments arranged by size:
454 312 220 217
Bsp1286I G_dGCh'C
Cuts at: 0 279 351 454 524 674 986 1203
Size: 279 72 103 70 150 312 217
Fragments arranged by size:
312 279 217 150 103 72 70
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 473 1203
Size: 473 730
BsrBI CCG'CTC
Cuts at: 0 48 711 1203
Size: 48 663 492
Fragments arranged by size:
663 492 48
BsrDI GCAATG_nn'
Cuts at: 0 420 641 944 1203
Size: 420 221 303 259
Fragments arranged by size:
420 303 259 221
BsrFI r'CCGG_y
Cuts at: 0 96 1148 1203
Size: 96 1052 55
Fragments arranged by size:
1052 96 55
BstYI r'GATC_y
Cuts at: 0 120 296 500 1203
Size: 120 176 204 703
Fragments arranged by size:
703 204 176 120
Bsu36I CC'TnA_GG
Cuts at: 0 173 1203
Size: 173 1030
BtgI C'CryG_G
Cuts at: 0 70 116 1203
Size: 70 46 1087
Fragments arranged by size:
1087 70 46
BtsI GCAGTG_nn'
Cuts at: 0 263 1203
Size: 263 940
DrdI GACnn_nn'nnGTC
Cuts at: 0 1092 1203
Size: 1092 111
EaeI y'GGCC_r
Cuts at: 0 131 196 260 737 1203
Size: 131 65 64 477 466
Fragments arranged by size:
477 466 131 65 64
EagI C'GGCC_G
Cuts at: 0 131 196 1203
Size: 131 65 1007
Fragments arranged by size:
1007 131 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 232 919 1203
Size: 232 687 284
Fragments arranged by size:
687 284 232
EcoICRI GAG'CTC
Cuts at: 0 452 984 1203
Size: 452 532 219
Fragments arranged by size:
532 452 219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 893 1203
Size: 703 190 310
Fragments arranged by size:
703 310 190
EcoO109I rG'GnC_Cy
Cuts at: 0 138 275 348 678 992 1203
Size: 138 137 73 330 314 211
Fragments arranged by size:
330 314 211 138 137 73
EcoRI G'AATT_C
Cuts at: 0 340 1203
Size: 340 863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 971 1131 1203
Size: 971 160 72
Fragments arranged by size:
971 160 72
HaeII r_GCGC'y
Cuts at: 0 23 79 334 372 641 1039 1203
Size: 23 56 255 38 269 398 164
Fragments arranged by size:
398 269 255 164 56 38 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 485 517 1203
Size: 485 32 686
Fragments arranged by size:
686 485 32
KasI G'GCGC_C
Cuts at: 0 19 75 368 1203
Size: 19 56 293 835
Fragments arranged by size:
835 293 56 19
MspA1I CmG'CkG
Cuts at: 0 72 272 302 630 1203
Size: 72 200 30 328 573
Fragments arranged by size:
573 328 200 72 30
NaeI GCC'GGC
Cuts at: 0 98 1203
Size: 98 1105
NarI GG'CG_CC
Cuts at: 0 20 76 369 1203
Size: 20 56 293 834
Fragments arranged by size:
834 293 56 20
NcoI C'CATG_G
Cuts at: 0 116 1203
Size: 116 1087
NgoMIV G'CCGG_C
Cuts at: 0 96 1203
Size: 96 1107
NotI GC'GGCC_GC
Cuts at: 0 196 1203
Size: 196 1007
NspI r_CATG'y
Cuts at: 0 1196 1203
Size: 1196 7
PasI CC'CwG_GG
Cuts at: 0 918 1203
Size: 918 285
PciI A'CATG_T
Cuts at: 0 1192 1203
Size: 1192 11
PfoI T'CCnGG_A
Cuts at: 0 586 1203
Size: 586 617
PpuMI rG'GwC_Cy
Cuts at: 0 678 992 1203
Size: 678 314 211
Fragments arranged by size:
678 314 211
PspOMI G'GGCC_C
Cuts at: 0 275 347 1203
Size: 275 72 856
Fragments arranged by size:
856 275 72
PstI C_TGCA'G
Cuts at: 0 410 1203
Size: 410 793
PvuII CAG'CTG
Cuts at: 0 630 1203
Size: 630 573
SacI G_AGCT'C
Cuts at: 0 454 986 1203
Size: 454 532 217
Fragments arranged by size:
532 454 217
SacII CC_GC'GG
Cuts at: 0 73 1203
Size: 73 1130
ScaI AGT'ACT
Cuts at: 0 829 1203
Size: 829 374
SexAI A'CCwGG_T
Cuts at: 0 1184 1203
Size: 1184 19
SfcI C'TryA_G
Cuts at: 0 406 625 1203
Size: 406 219 578
Fragments arranged by size:
578 406 219
SfoI GGC'GCC
Cuts at: 0 21 77 370 1203
Size: 21 56 293 833
Fragments arranged by size:
833 293 56 21
SmaI CCC'GGG
Cuts at: 0 112 143 280 1203
Size: 112 31 137 923
Fragments arranged by size:
923 137 112 31
SmlI C'TyrA_G
Cuts at: 0 361 1203
Size: 361 842
SrfI GCCC'GGGC
Cuts at: 0 112 1203
Size: 112 1091
StuI AGG'CCT
Cuts at: 0 151 1203
Size: 151 1052
StyI C'CwwG_G
Cuts at: 0 116 899 1203
Size: 116 783 304
Fragments arranged by size:
783 304 116
TatI w'GTAC_w
Cuts at: 0 827 1203
Size: 827 376
Tth111I GACn'n_nGTC
Cuts at: 0 1186 1203
Size: 1186 17
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 123 732 1203
Size: 123 609 471
Fragments arranged by size:
609 471 123
XmaI C'CCGG_G
Cuts at: 0 110 141 278 1203
Size: 110 31 137 925
Fragments arranged by size:
925 137 110 31
Enzymes that do cut:
AcuI AflIII ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbvCI BglI Bme1580I BmrI Bpu10I BpuEI
BsaI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BstYI Bsu36I BtgI
BtsI DrdI EaeI EagI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I KasI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PasI PciI PfoI PpuMI
PspOMI PstI PvuII SacI SacII ScaI SexAI SfcI
SfoI SmaI SmlI SrfI StuI StyI TatI Tth111I
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI AscI AseI AsiSI
AvrII BaeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BplI BpmI BsaAI BsaBI BsaXI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI
DraI DraIII EarI EcoNI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI NdeI NheI NruI NsiI PacI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SapI SbfI SfiI SgfI SgrAI SnaBI
SpeI SphI SspI SwaI TaqII TstI XbaI XhoI
XmnI ZraI