Restriction map for
pF1KE0698
(Linear) (Six-base) MAPSORT of: KIEE0698.seq Check: 6709 from: 1 to: 2289
KIEE0698.orf
With 182 enzymes: SgfI *
February 2, 2010 10:44 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 2137 2194 2289
Size: 598 1539 57 95
Fragments arranged by size:
1539 598 95 57
AflIII A'CryG_T
Cuts at: 0 1182 2289
Size: 1182 1107
AleI CACnn'nnGTG
Cuts at: 0 562 1108 2289
Size: 562 546 1181
Fragments arranged by size:
1181 562 546
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1735 2289
Size: 1735 554
AlwNI CAG_nnn'CTG
Cuts at: 0 960 978 2031 2289
Size: 960 18 1053 258
Fragments arranged by size:
1053 960 258 18
ApoI r'AATT_y
Cuts at: 0 289 500 703 1290 1949 2289
Size: 289 211 203 587 659 340
Fragments arranged by size:
659 587 340 289 211 203
AseI AT'TA_AT
Cuts at: 0 1481 2270 2289
Size: 1481 789 19
Fragments arranged by size:
1481 789 19
AvaI C'yCGr_G
Cuts at: 0 177 2289
Size: 177 2112
BanI G'GyrC_C
Cuts at: 0 787 1821 2289
Size: 787 1034 468
Fragments arranged by size:
1034 787 468
BanII G_rGCy'C
Cuts at: 0 115 920 2289
Size: 115 805 1369
Fragments arranged by size:
1369 805 115
BbeI G_GCGC'C
Cuts at: 0 791 2289
Size: 791 1498
BbsI GAAGACnn'nnnn_
Cuts at: 0 536 739 1106 1663 1936 2289
Size: 536 203 367 557 273 353
Fragments arranged by size:
557 536 367 353 273 203
BbvCI CC'TCA_GC
Cuts at: 0 1676 2289
Size: 1676 613
BglII A'GATC_T
Cuts at: 0 1063 2289
Size: 1063 1226
BlpI GC'TnA_GC
Cuts at: 0 971 2289
Size: 971 1318
Bme1580I G_kGCm'C
Cuts at: 0 628 2289
Size: 628 1661
BmgBI CAC'GTC
Cuts at: 0 1995 2289
Size: 1995 294
BmrI ACTGGGnnnn_n'
Cuts at: 0 1251 2289
Size: 1251 1038
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 590 1662 2289
Size: 590 1072 627
Fragments arranged by size:
1072 627 590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 1601 1883 1960 2289
Size: 118 1483 282 77 329
Fragments arranged by size:
1483 329 282 118 77
Bpu10I CC'TnA_GC
Cuts at: 0 1676 2289
Size: 1676 613
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1520 2083 2289
Size: 1520 563 206
Fragments arranged by size:
1520 563 206
BsaI GGTCTCn'nnnn_
Cuts at: 0 1313 2289
Size: 1313 976
BsaAI yAC'GTr
Cuts at: 0 432 1183 2289
Size: 432 751 1106
Fragments arranged by size:
1106 751 432
BsaBI GATnn'nnATC
Cuts at: 0 479 599 2289
Size: 479 120 1690
Fragments arranged by size:
1690 479 120
BsaHI Gr'CG_yC
Cuts at: 0 788 2289
Size: 788 1501
BsaWI w'CCGG_w
Cuts at: 0 887 1305 2128 2289
Size: 887 418 823 161
Fragments arranged by size:
887 823 418 161
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 129 159 2289
Size: 129 30 2130
Fragments arranged by size:
2130 129 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 2179 2289
Size: 292 1887 110
Fragments arranged by size:
1887 292 110
BseYI C'CCAG_C
Cuts at: 0 779 1013 1402 2039 2289
Size: 779 234 389 637 250
Fragments arranged by size:
779 637 389 250 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 2289
Size: 892 1397
BsmI GAATG_Cn'
Cuts at: 0 576 1362 2289
Size: 576 786 927
Fragments arranged by size:
927 786 576
Bsp1286I G_dGCh'C
Cuts at: 0 115 628 920 2289
Size: 115 513 292 1369
Fragments arranged by size:
1369 513 292 115
BspEI T'CCGG_A
Cuts at: 0 887 2289
Size: 887 1402
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 914 2289
Size: 914 1375
BsrDI GCAATG_nn'
Cuts at: 0 340 2289
Size: 340 1949
BstAPI GCAn_nnn'nTGC
Cuts at: 0 162 1546 1555 2289
Size: 162 1384 9 734
Fragments arranged by size:
1384 734 162 9
BstYI r'GATC_y
Cuts at: 0 1063 1608 2289
Size: 1063 545 681
Fragments arranged by size:
1063 681 545
Bsu36I CC'TnA_GG
Cuts at: 0 202 2289
Size: 202 2087
BtgI C'CryG_G
Cuts at: 0 867 1370 1728 1876 1886 2106 2289
Size: 867 503 358 148 10 220 183
Fragments arranged by size:
867 503 358 220 183 148 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1154 2289
Size: 1154 1135
BtsI GCAGTG_nn'
Cuts at: 0 1001 2289
Size: 1001 1288
DraI TTT'AAA
Cuts at: 0 170 2289
Size: 170 2119
EaeI y'GGCC_r
Cuts at: 0 614 807 1367 1936 2289
Size: 614 193 560 569 353
Fragments arranged by size:
614 569 560 353 193
EarI CTCTTCn'nnn_
Cuts at: 0 117 902 1229 2009 2156 2180 2234 2289
Size: 117 785 327 780 147 24 54 55
Fragments arranged by size:
785 780 327 147 117 55 54 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 598 1601 1883 1960 2137 2194 2289
Size: 118 480 1003 282 77 177 57 95
Fragments arranged by size:
1003 480 282 177 118 95 77 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1228 2289
Size: 1228 1061
EcoO109I rG'GnC_Cy
Cuts at: 0 1239 1375 1861 2289
Size: 1239 136 486 428
Fragments arranged by size:
1239 486 428 136
FspI TGC'GCA
Cuts at: 0 857 2289
Size: 857 1432
HaeII r_GCGC'y
Cuts at: 0 791 2289
Size: 791 1498
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1145 1177 1848 1880 2289
Size: 1145 32 671 32 409
Fragments arranged by size:
1145 671 409 32 32
HincII GTy'rAC
Cuts at: 0 951 2289
Size: 951 1338
KasI G'GCGC_C
Cuts at: 0 787 2289
Size: 787 1502
MfeI C'AATT_G
Cuts at: 0 943 2289
Size: 943 1346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 381 2289
Size: 381 1908
MscI TGG'CCA
Cuts at: 0 616 809 2289
Size: 616 193 1480
Fragments arranged by size:
1480 616 193
MslI CAynn'nnrTG
Cuts at: 0 562 1108 1727 2289
Size: 562 546 619 562
Fragments arranged by size:
619 562 562 546
MspA1I CmG'CkG
Cuts at: 0 327 960 975 2088 2289
Size: 327 633 15 1113 201
Fragments arranged by size:
1113 633 327 201 15
NarI GG'CG_CC
Cuts at: 0 788 2289
Size: 788 1501
NcoI C'CATG_G
Cuts at: 0 867 1370 1728 2289
Size: 867 503 358 561
Fragments arranged by size:
867 561 503 358
NspI r_CATG'y
Cuts at: 0 286 2289
Size: 286 2003
PasI CC'CwG_GG
Cuts at: 0 913 2289
Size: 913 1376
PflMI CCAn_nnn'nTGG
Cuts at: 0 960 2289
Size: 960 1329
PmlI CAC'GTG
Cuts at: 0 1183 2289
Size: 1183 1106
PpuMI rG'GwC_Cy
Cuts at: 0 1239 1375 2289
Size: 1239 136 914
Fragments arranged by size:
1239 914 136
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 652 684 2074 2106 2289
Size: 652 32 1390 32 183
Fragments arranged by size:
1390 652 183 32 32
PvuII CAG'CTG
Cuts at: 0 327 960 975 2289
Size: 327 633 15 1314
Fragments arranged by size:
1314 633 327 15
SapI GCTCTTCn'nnn_
Cuts at: 0 2234 2289
Size: 2234 55
ScaI AGT'ACT
Cuts at: 0 666 2289
Size: 666 1623
SfcI C'TryA_G
Cuts at: 0 452 2289
Size: 452 1837
SfoI GGC'GCC
Cuts at: 0 789 2289
Size: 789 1500
SmlI C'TyrA_G
Cuts at: 0 1535 2098 2289
Size: 1535 563 191
Fragments arranged by size:
1535 563 191
SphI G_CATG'C
Cuts at: 0 286 2289
Size: 286 2003
StyI C'CwwG_G
Cuts at: 0 617 867 1370 1728 1964 2289
Size: 617 250 503 358 236 325
Fragments arranged by size:
617 503 358 325 250 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1337 1416 2289
Size: 1337 79 873
Fragments arranged by size:
1337 873 79
TatI w'GTAC_w
Cuts at: 0 139 664 2057 2289
Size: 139 525 1393 232
Fragments arranged by size:
1393 525 232 139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 129 161 2289
Size: 129 32 2128
Fragments arranged by size:
2128 129 32
Tth111I GACn'n_nGTC
Cuts at: 0 2031 2289
Size: 2031 258
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApoI AseI AvaI
BanI BanII BbeI BbsI BbvCI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsmI
Bsp1286I BspEI BspMI BsrDI BstAPI BstYI Bsu36I BtgI
BtgZI BtsI DraI EaeI EarI Eco57MI EcoNI EcoO109I
FspI HaeII Hin4I HincII KasI MfeI MmeI MscI
MslI MspA1I NarI NcoI NspI PasI PflMI PmlI
PpuMI PsrI PvuII SapI ScaI SfcI SfoI SmlI
SphI StyI TaqII TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BfrBI BglI BmtI BsiEI
BsiHKAI BsiWI BsmBI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I ClaI CspCI DraIII
DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI
FspAI HindIII HpaI KpnI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PpiI PshAI PsiI PspOMI PspXI PstI PvuI RsrII
SacI SacII SalI SanDI SbfI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
XbaI XcmI XhoI XmaI XmnI ZraI