Restriction map for
pF1KE0699
(Linear) (Six-base) MAPSORT of: KIEE0699.seq Check: 5982 from: 1 to: 13689
KIEE0699.orf
With 182 enzymes: SgfI *
May 18, 2010 14:02 ..
AatII G_ACGT'C
Cuts at: 0 9869 13689
Size: 9869 3820
AccI GT'mk_AC
Cuts at: 0 4085 5162 7049 9408 11666 13689
Size: 4085 1077 1887 2359 2258 2023
Fragments arranged by size:
4085 2359 2258 2023 1887 1077
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 358 1618 1872 2033 2785 3118 3697
Size: 295 63 1260 254 161 752 333 579
Cuts at: 3697 3793 4385 4818 5052 5476 5612 6316 6993
Size: 96 592 433 234 424 136 704 677
Cuts at: 6993 7409 7795 7804 8107 8491 10568 11251 11277
Size: 416 386 9 303 384 2077 683 26
Cuts at: 11277 11396 11844 11884 12880 12993 13084 13689
Size: 119 448 40 996 113 91 605
Fragments arranged by size:
2077 1260 996 752 704 683 677 605
592 579 448 433 424 416 386 384
333 303 295 254 234 161 136 119
113 96 91 63 40 26 9
AflII C'TTAA_G
Cuts at: 0 5206 6805 13252 13689
Size: 5206 1599 6447 437
Fragments arranged by size:
6447 5206 1599 437
AflIII A'CryG_T
Cuts at: 0 1197 7262 12509 13689
Size: 1197 6065 5247 1180
Fragments arranged by size:
6065 5247 1197 1180
AleI CACnn'nnGTG
Cuts at: 0 12880 13689
Size: 12880 809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 349 3635 9284 12347 13689
Size: 349 3286 5649 3063 1342
Fragments arranged by size:
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AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2866 2898 9740 9772 13689
Size: 2866 32 6842 32 3917
Fragments arranged by size:
6842 3917 2866 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 993 1191 1275 1386 1590 2382 5563 7368
Size: 993 198 84 111 204 792 3181 1805
Cuts at: 7368 8037 12190 12450 12462 13689
Size: 669 4153 260 12 1227
Fragments arranged by size:
4153 3181 1805 1227 993 792 669 260
204 198 111 84 12
ApoI r'AATT_y
Cuts at: 0 1736 1894 3194 3523 4285 4306 4857 5086
Size: 1736 158 1300 329 762 21 551 229
Cuts at: 5086 5248 5788 6151 6378 6660 7252 7358 7531
Size: 162 540 363 227 282 592 106 173
Cuts at: 7531 7945 8158 8218 8960 9102 9162 9300 9478
Size: 414 213 60 742 142 60 138 178
Cuts at: 9478 9740 10210 10295 10314 10375 10563 10644 11185
Size: 262 470 85 19 61 188 81 541
Cuts at: 11185 11220 11368 12541 12961 13081 13534 13689
Size: 35 148 1173 420 120 453 155
Fragments arranged by size:
1736 1300 1173 762 742 592 551 541
540 470 453 420 414 363 329 282
262 229 227 213 188 178 173 162
158 155 148 142 138 120 106 85
81 61 60 60 35 21 19
AseI AT'TA_AT
Cuts at: 0 3688 4907 8456 9889 12977 13689
Size: 3688 1219 3549 1433 3088 712
Fragments arranged by size:
3688 3549 3088 1433 1219 712
AvaI C'yCGr_G
Cuts at: 0 1255 3995 10517 11359 13689
Size: 1255 2740 6522 842 2330
Fragments arranged by size:
6522 2740 2330 1255 842
AvrII C'CTAG_G
Cuts at: 0 9855 13689
Size: 9855 3834
BamHI G'GATC_C
Cuts at: 0 2423 4521 10987 13689
Size: 2423 2098 6466 2702
Fragments arranged by size:
6466 2702 2423 2098
BanI G'GyrC_C
Cuts at: 0 70 2404 3541 4585 5935 10147 12019 12406
Size: 70 2334 1137 1044 1350 4212 1872 387
Cuts at: 12406 13689
Size: 1283
Fragments arranged by size:
4212 2334 1872 1350 1283 1137 1044 387
70
BanII G_rGCy'C
Cuts at: 0 392 1262 2461 3065 3706 4161 5233 6098
Size: 392 870 1199 604 641 455 1072 865
Cuts at: 6098 9725 12284 13689
Size: 3627 2559 1405
Fragments arranged by size:
3627 2559 1405 1199 1072 870 865 641
604 455 392
BbeI G_GCGC'C
Cuts at: 0 74 13689
Size: 74 13615
BbsI GAAGACnn'nnnn_
Cuts at: 0 283 872 985 1514 3724 5676 7801 10574
Size: 283 589 113 529 2210 1952 2125 2773
Cuts at: 10574 11417 12293 13689
Size: 843 876 1396
Fragments arranged by size:
2773 2210 2125 1952 1396 876 843 589
529 283 113
BbvCI CC'TCA_GC
Cuts at: 0 1263 1953 8346 11997 13689
Size: 1263 690 6393 3651 1692
Fragments arranged by size:
6393 3651 1692 1263 690
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 619 653 13689
Size: 619 34 13036
Fragments arranged by size:
13036 619 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 531 3084 6750 9742 13689
Size: 531 2553 3666 2992 3947
Fragments arranged by size:
3947 3666 2992 2553 531
BclI T'GATC_A
Cuts at: 0 713 6312 6543 13229 13689
Size: 713 5599 231 6686 460
Fragments arranged by size:
6686 5599 713 460 231
BfrBI ATG'CAT
Cuts at: 0 4369 9643 13689
Size: 4369 5274 4046
Fragments arranged by size:
5274 4369 4046
BglI GCCn_nnn'nGGC
Cuts at: 0 1585 13689
Size: 1585 12104
BglII A'GATC_T
Cuts at: 0 1283 1827 3924 11262 13689
Size: 1283 544 2097 7338 2427
Fragments arranged by size:
7338 2427 2097 1283 544
BlpI GC'TnA_GC
Cuts at: 0 1333 5924 7129 10441 13689
Size: 1333 4591 1205 3312 3248
Fragments arranged by size:
4591 3312 3248 1333 1205
Bme1580I G_kGCm'C
Cuts at: 0 2409 5910 9246 10150 10603 12224 13689
Size: 2409 3501 3336 904 453 1621 1465
Fragments arranged by size:
3501 3336 2409 1621 1465 904 453
BmgBI CAC'GTC
Cuts at: 0 12381 13689
Size: 12381 1308
BmrI ACTGGGnnnn_n'
Cuts at: 0 1445 2169 4333 5137 5780 9062 9233 10814
Size: 1445 724 2164 804 643 3282 171 1581
Cuts at: 10814 11463 12276 12422 13689
Size: 649 813 146 1267
Fragments arranged by size:
3282 2164 1581 1445 1267 813 804 724
649 643 171 146
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1054 2344 11610 11685 13689
Size: 1054 1290 9266 75 2004
Fragments arranged by size:
9266 2004 1290 1054 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 970 1168 1493 2359 2540 2573 2600
Size: 265 705 198 325 866 181 33 27
Cuts at: 2600 2752 3049 3772 3903 4241 5020 5903 8475
Size: 152 297 723 131 338 779 883 2572
Cuts at: 8475 8620 10673 12077 13649 13689
Size: 145 2053 1404 1572 40
Fragments arranged by size:
2572 2053 1572 1404 883 866 779 723
705 338 325 297 265 198 181 152
145 131 40 33 27
Bpu10I CC'TnA_GC
Cuts at: 0 1263 1953 5570 8346 11573 11997 13418 13689
Size: 1263 690 3617 2776 3227 424 1421 271
Fragments arranged by size:
3617 3227 2776 1421 1263 690 424 271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1501 3095 4891 5932 6961 9922 12484
Size: 376 1125 1594 1796 1041 1029 2961 2562
Cuts at: 12484 12521 12853 13097 13293 13689
Size: 37 332 244 196 396
Fragments arranged by size:
2961 2562 1796 1594 1125 1041 1029 396
376 332 244 196 37
BsaI GGTCTCn'nnnn_
Cuts at: 0 614 2784 2838 4277 6217 7553 13689
Size: 614 2170 54 1439 1940 1336 6136
Fragments arranged by size:
6136 2170 1940 1439 1336 614 54
BsaAI yAC'GTr
Cuts at: 0 3675 12889 13689
Size: 3675 9214 800
Fragments arranged by size:
9214 3675 800
BsaBI GATnn'nnATC
Cuts at: 0 8072 11957 13600 13689
Size: 8072 3885 1643 89
Fragments arranged by size:
8072 3885 1643 89
BsaHI Gr'CG_yC
Cuts at: 0 71 9866 13689
Size: 71 9795 3823
Fragments arranged by size:
9795 3823 71
BsaWI w'CCGG_w
Cuts at: 0 146 2673 3198 10910 13689
Size: 146 2527 525 7712 2779
Fragments arranged by size:
7712 2779 2527 525 146
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 936 966 2938 2953 2968 2983 3425 3455
Size: 936 30 1972 15 15 15 442 30
Cuts at: 3455 3944 3974 5827 5857 8606 8636 9417 9447
Size: 489 30 1853 30 2749 30 781 30
Cuts at: 9447 11339 11369 11685 11715 13689
Size: 1892 30 316 30 1974
Fragments arranged by size:
2749 1974 1972 1892 1853 936 781 489
442 316 30 30 30 30 30 30
30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 373 1713 2359 4560 8288 9422 13689
Size: 373 1340 646 2201 3728 1134 4267
Fragments arranged by size:
4267 3728 2201 1340 1134 646 373
BseYI C'CCAG_C
Cuts at: 0 254 1947 2755 5617 8981 9122 9245 11940
Size: 254 1693 808 2862 3364 141 123 2695
Cuts at: 11940 12009 13689
Size: 69 1680
Fragments arranged by size:
3364 2862 2695 1693 1680 808 254 141
123 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1416 2910 5903 11636 11885 13315 13689
Size: 1416 1494 2993 5733 249 1430 374
Fragments arranged by size:
5733 2993 1494 1430 1416 374 249
BsiHKAI G_wGCw'C
Cuts at: 0 392 937 1171 3065 3706 4920 5647 6644
Size: 392 545 234 1894 641 1214 727 997
Cuts at: 6644 9725 10576 10694 13689
Size: 3081 851 118 2995
Fragments arranged by size:
3081 2995 1894 1214 997 851 727 641
545 392 234 118
BsmI GAATG_Cn'
Cuts at: 0 2505 5019 9549 9805 10872 13689
Size: 2505 2514 4530 256 1067 2817
Fragments arranged by size:
4530 2817 2514 2505 1067 256
Bsp1286I G_dGCh'C
Cuts at: 0 392 937 1171 1262 2409 2461 3065 3706
Size: 392 545 234 91 1147 52 604 641
Cuts at: 3706 4161 4920 5233 5647 5910 6098 6644 9246
Size: 455 759 313 414 263 188 546 2602
Cuts at: 9246 9725 10150 10576 10603 10694 12224 12284 13689
Size: 479 425 426 27 91 1530 60 1405
Fragments arranged by size:
2602 1530 1405 1147 759 641 604 546
545 479 455 426 425 414 392 313
263 234 188 91 91 60 52 27
BspEI T'CCGG_A
Cuts at: 0 146 2673 3198 10910 13689
Size: 146 2527 525 7712 2779
Fragments arranged by size:
7712 2779 2527 525 146
BspHI T'CATG_A
Cuts at: 0 5829 13640 13689
Size: 5829 7811 49
Fragments arranged by size:
7811 5829 49
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 408 4202 8350 8723 11622 13689
Size: 408 3794 4148 373 2899 2067
Fragments arranged by size:
4148 3794 2899 2067 408 373
BsrDI GCAATG_nn'
Cuts at: 0 597 1394 2558 3704 5273 5685 5745 7140
Size: 597 797 1164 1146 1569 412 60 1395
Cuts at: 7140 10979 11501 13442 13689
Size: 3839 522 1941 247
Fragments arranged by size:
3839 1941 1569 1395 1164 1146 797 597
522 412 247 60
BsrFI r'CCGG_y
Cuts at: 0 4535 13689
Size: 4535 9154
BsrGI T'GTAC_A
Cuts at: 0 4097 11126 13689
Size: 4097 7029 2563
Fragments arranged by size:
7029 4097 2563
BssSI C'ACGA_G
Cuts at: 0 2719 5860 7556 13689
Size: 2719 3141 1696 6133
Fragments arranged by size:
6133 3141 2719 1696
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3121 11650 13329 13689
Size: 3121 8529 1679 360
Fragments arranged by size:
8529 3121 1679 360
BstBI TT'CG_AA
Cuts at: 0 4297 12697 13689
Size: 4297 8400 992
Fragments arranged by size:
8400 4297 992
BstEII G'GTnAC_C
Cuts at: 0 1230 5473 13689
Size: 1230 4243 8216
Fragments arranged by size:
8216 4243 1230
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2437 3492 5154 13689
Size: 2437 1055 1662 8535
Fragments arranged by size:
8535 2437 1662 1055
BstYI r'GATC_y
Cuts at: 0 1283 1827 2423 3407 3585 3924 4216 4521
Size: 1283 544 596 984 178 339 292 305
Cuts at: 4521 5418 8082 10987 11262 11514 13127 13689
Size: 897 2664 2905 275 252 1613 562
Fragments arranged by size:
2905 2664 1613 1283 984 897 596 562
544 339 305 292 275 252 178
Bsu36I CC'TnA_GG
Cuts at: 0 320 2931 4558 11005 13689
Size: 320 2611 1627 6447 2684
Fragments arranged by size:
6447 2684 2611 1627 320
BtgI C'CryG_G
Cuts at: 0 1493 1744 4863 9998 10370 10777 12023 13689
Size: 1493 251 3119 5135 372 407 1246 1666
Fragments arranged by size:
5135 3119 1666 1493 1246 407 372 251
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3857 5598 13689
Size: 3857 1741 8091
Fragments arranged by size:
8091 3857 1741
BtsI GCAGTG_nn'
Cuts at: 0 637 2030 4786 5665 7439 8059 8378 10126
Size: 637 1393 2756 879 1774 620 319 1748
Cuts at: 10126 13412 13689
Size: 3286 277
Fragments arranged by size:
3286 2756 1774 1748 1393 879 637 620
319 277
ClaI AT'CG_AT
Cuts at: 0 5720 13689
Size: 5720 7969
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3313 3348 8549 8584 11067 11102 13689
Size: 3313 35 5201 35 2483 35 2587
Fragments arranged by size:
5201 3313 2587 2483 35 35 35
DraI TTT'AAA
Cuts at: 0 4709 5390 7227 9074 10029 12852 13035 13689
Size: 4709 681 1837 1847 955 2823 183 654
Fragments arranged by size:
4709 2823 1847 1837 955 681 654 183
DraIII CAC_nnn'GTG
Cuts at: 0 11854 13689
Size: 11854 1835
DrdI GACnn_nn'nnGTC
Cuts at: 0 12681 13689
Size: 12681 1008
EaeI y'GGCC_r
Cuts at: 0 398 3846 4533 4941 5061 8748 10848 13689
Size: 398 3448 687 408 120 3687 2100 2841
Fragments arranged by size:
3687 3448 2841 2100 687 408 398 120
EarI CTCTTCn'nnn_
Cuts at: 0 77 802 1045 1937 3506 3835 4482 5304
Size: 77 725 243 892 1569 329 647 822
Cuts at: 5304 5532 7823 9953 10368 12173 13689
Size: 228 2291 2130 415 1805 1516
Fragments arranged by size:
2291 2130 1805 1569 1516 892 822 725
647 415 329 243 228 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2955 4098 13689
Size: 2955 1143 9591
Fragments arranged by size:
9591 2955 1143
EcoICRI GAG'CTC
Cuts at: 0 390 3063 3704 9723 13689
Size: 390 2673 641 6019 3966
Fragments arranged by size:
6019 3966 2673 641 390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 295 358 970 1168 1493 1618 1872
Size: 265 30 63 612 198 325 125 254
Cuts at: 1872 2033 2359 2540 2573 2600 2752 2785 3049
Size: 161 326 181 33 27 152 33 264
Cuts at: 3049 3118 3697 3772 3793 3903 4241 4385 4818
Size: 69 579 75 21 110 338 144 433
Cuts at: 4818 5020 5052 5476 5612 5903 6316 6993 7409
Size: 202 32 424 136 291 413 677 416
Cuts at: 7409 7795 7804 8107 8475 8491 8620 10568 10673
Size: 386 9 303 368 16 129 1948 105
Cuts at: 10673 11251 11277 11396 11844 11884 12077 12880 12993
Size: 578 26 119 448 40 193 803 113
Cuts at: 12993 13084 13649 13689
Size: 91 565 40
Fragments arranged by size:
1948 803 677 612 579 578 565 448
433 424 416 413 386 368 338 326
325 303 291 265 264 254 202 198
193 181 161 152 144 136 129 125
119 113 110 105 91 75 69 63
40 40 33 33 32 30 27 26
21 16 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 298 9612 10849 13689
Size: 298 9314 1237 2840
Fragments arranged by size:
9314 2840 1237 298
EcoO109I rG'GnC_Cy
Cuts at: 0 1376 8854 9731 10722 12237 12416 12900 13689
Size: 1376 7478 877 991 1515 179 484 789
Fragments arranged by size:
7478 1515 1376 991 877 789 484 179
EcoRI G'AATT_C
Cuts at: 0 6378 8158 8218 9478 10563 12541 13689
Size: 6378 1780 60 1260 1085 1978 1148
Fragments arranged by size:
6378 1978 1780 1260 1148 1085 60
EcoRV GAT'ATC
Cuts at: 0 1821 10620 12375 13689
Size: 1821 8799 1755 1314
Fragments arranged by size:
8799 1821 1755 1314
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1990 2487 2922 5221 6097 7825 9222 9498
Size: 1990 497 435 2299 876 1728 1397 276
Cuts at: 9498 10461 13689
Size: 963 3228
Fragments arranged by size:
3228 2299 1990 1728 1397 963 876 497
435 276
FspI TGC'GCA
Cuts at: 0 8719 13689
Size: 8719 4970
HaeII r_GCGC'y
Cuts at: 0 31 40 74 2939 13689
Size: 31 9 34 2865 10750
Fragments arranged by size:
10750 2865 34 31 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1020 1052 1686 1718 1936 1968 2173 2205
Size: 1020 32 634 32 218 32 205 32
Cuts at: 2205 2281 2313 2432 2464 2847 2879 2953 2985
Size: 76 32 119 32 383 32 74 32
Cuts at: 2985 4804 4836 4939 4971 5195 5227 6184 6216
Size: 1819 32 103 32 224 32 957 32
Cuts at: 6216 7004 7036 8542 8574 8606 8619 8638 8651
Size: 788 32 1506 32 32 13 19 13
Cuts at: 8651 11085 11117 12303 12335 12781 12813 13209 13241
Size: 2434 32 1186 32 446 32 396 32
Cuts at: 13241 13501 13533 13689
Size: 260 32 156
Fragments arranged by size:
2434 1819 1506 1186 1020 957 788 634
446 396 383 260 224 218 205 156
119 103 76 74 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
19 13 13
HincII GTy'rAC
Cuts at: 0 707 1347 1505 2145 3213 4191 5163 7248
Size: 707 640 158 640 1068 978 972 2085
Cuts at: 7248 9191 10431 11390 11459 12255 12467 13689
Size: 1943 1240 959 69 796 212 1222
Fragments arranged by size:
2085 1943 1240 1222 1068 978 972 959
796 707 640 640 212 158 69
HindIII A'AGCT_T
Cuts at: 0 2150 7207 9940 10438 13106 13657 13689
Size: 2150 5057 2733 498 2668 551 32
Fragments arranged by size:
5057 2733 2668 2150 551 498 32
HpaI GTT'AAC
Cuts at: 0 1505 7248 9191 11390 11459 13689
Size: 1505 5743 1943 2199 69 2230
Fragments arranged by size:
5743 2230 2199 1943 1505 69
KasI G'GCGC_C
Cuts at: 0 70 13689
Size: 70 13619
MfeI C'AATT_G
Cuts at: 0 6158 6387 6898 7479 7985 8246 13689
Size: 6158 229 511 581 506 261 5443
Fragments arranged by size:
6158 5443 581 511 506 261 229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1897 2969 4577 7161 10692 12945 13689
Size: 1897 1072 1608 2584 3531 2253 744
Fragments arranged by size:
3531 2584 2253 1897 1608 1072 744
MscI TGG'CCA
Cuts at: 0 400 4943 8750 10850 13689
Size: 400 4543 3807 2100 2839
Fragments arranged by size:
4543 3807 2839 2100 400
MslI CAynn'nnrTG
Cuts at: 0 2491 3506 3617 4260 5516 5638 5732 6653
Size: 2491 1015 111 643 1256 122 94 921
Cuts at: 6653 8519 8787 9074 9796 11105 11566 12034 12880
Size: 1866 268 287 722 1309 461 468 846
Cuts at: 12880 13639 13689
Size: 759 50
Fragments arranged by size:
2491 1866 1309 1256 1015 921 846 759
722 643 468 461 287 268 122 111
94 50
MspA1I CmG'CkG
Cuts at: 0 1107 1275 2053 2982 5778 5923 8463 12948
Size: 1107 168 778 929 2796 145 2540 4485
Cuts at: 12948 13689
Size: 741
Fragments arranged by size:
4485 2796 2540 1107 929 778 741 168
145
NaeI GCC'GGC
Cuts at: 0 4537 13689
Size: 4537 9152
NarI GG'CG_CC
Cuts at: 0 71 13689
Size: 71 13618
NcoI C'CATG_G
Cuts at: 0 1493 1744 4863 9998 10370 10777 13689
Size: 1493 251 3119 5135 372 407 2912
Fragments arranged by size:
5135 3119 2912 1493 407 372 251
NdeI CA'TA_TG
Cuts at: 0 3614 4233 4500 5235 12548 13026 13689
Size: 3614 619 267 735 7313 478 663
Fragments arranged by size:
7313 3614 735 663 619 478 267
NgoMIV G'CCGG_C
Cuts at: 0 4535 13689
Size: 4535 9154
NsiI A_TGCA'T
Cuts at: 0 4371 9645 13689
Size: 4371 5274 4044
Fragments arranged by size:
5274 4371 4044
NspI r_CATG'y
Cuts at: 0 766 1207 2016 2607 5467 6412 6949 7266
Size: 766 441 809 591 2860 945 537 317
Cuts at: 7266 8989 9840 11750 13689
Size: 1723 851 1910 1939
Fragments arranged by size:
2860 1939 1910 1723 945 851 809 766
591 537 441 317
PasI CC'CwG_GG
Cuts at: 0 193 6342 13689
Size: 193 6149 7347
Fragments arranged by size:
7347 6149 193
PciI A'CATG_T
Cuts at: 0 7262 13689
Size: 7262 6427
PflMI CCAn_nnn'nTGG
Cuts at: 0 1494 2382 3795 5985 8919 8988 10857 13689
Size: 1494 888 1413 2190 2934 69 1869 2832
Fragments arranged by size:
2934 2832 2190 1869 1494 1413 888 69
PfoI T'CCnGG_A
Cuts at: 0 986 1659 2309 2565 3632 6739 9744 11735
Size: 986 673 650 256 1067 3107 3005 1991
Cuts at: 11735 12134 13665 13689
Size: 399 1531 24
Fragments arranged by size:
3107 3005 1991 1531 1067 986 673 650
399 256 24
PmlI CAC'GTG
Cuts at: 0 3675 13689
Size: 3675 10014
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 7735 7767 13689
Size: 7735 32 5922
Fragments arranged by size:
7735 5922 32
PpuMI rG'GwC_Cy
Cuts at: 0 1376 8854 9731 12237 12416 12900 13689
Size: 1376 7478 877 2506 179 484 789
Fragments arranged by size:
7478 2506 1376 877 789 484 179
PsiI TTA'TAA
Cuts at: 0 5339 6964 13689
Size: 5339 1625 6725
Fragments arranged by size:
6725 5339 1625
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 12058 12090 13689
Size: 12058 32 1599
Fragments arranged by size:
12058 1599 32
PstI C_TGCA'G
Cuts at: 0 265 372 2420 6524 6800 8054 10133 10598
Size: 265 107 2048 4104 276 1254 2079 465
Cuts at: 10598 12326 13689
Size: 1728 1363
Fragments arranged by size:
4104 2079 2048 1728 1363 1254 465 276
265 107
PvuII CAG'CTG
Cuts at: 0 1107 1275 2053 5778 5923 12948 13689
Size: 1107 168 778 3725 145 7025 741
Fragments arranged by size:
7025 3725 1107 778 741 168 145
SacI G_AGCT'C
Cuts at: 0 392 3065 3706 9725 13689
Size: 392 2673 641 6019 3964
Fragments arranged by size:
6019 3964 2673 641 392
SalI G'TCGA_C
Cuts at: 0 5161 13689
Size: 5161 8528
SanDI GG'GwC_CC
Cuts at: 0 1376 13689
Size: 1376 12313
SapI GCTCTTCn'nnn_
Cuts at: 0 7823 13689
Size: 7823 5866
ScaI AGT'ACT
Cuts at: 0 6732 10236 13689
Size: 6732 3504 3453
Fragments arranged by size:
6732 3504 3453
SexAI A'CCwGG_T
Cuts at: 0 1238 13689
Size: 1238 12451
SfcI C'TryA_G
Cuts at: 0 261 368 1370 2416 3020 3461 4086 4116
Size: 261 107 1002 1046 604 441 625 30
Cuts at: 4116 5362 6520 6796 7450 8050 10129 10594 12075
Size: 1246 1158 276 654 600 2079 465 1481
Cuts at: 12075 12322 13689
Size: 247 1367
Fragments arranged by size:
2079 1481 1367 1246 1158 1046 1002 654
625 604 600 465 441 276 261 247
107 30
SfoI GGC'GCC
Cuts at: 0 72 13689
Size: 72 13617
SmlI C'TyrA_G
Cuts at: 0 391 1516 3110 3995 4870 5206 5947 6805
Size: 391 1125 1594 885 875 336 741 858
Cuts at: 6805 6940 9937 12499 12536 12868 13112 13252 13308
Size: 135 2997 2562 37 332 244 140 56
Cuts at: 13308 13689
Size: 381
Fragments arranged by size:
2997 2562 1594 1125 885 875 858 741
391 381 336 332 244 140 135 56
37
SnaBI TAC'GTA
Cuts at: 0 12889 13689
Size: 12889 800
SphI G_CATG'C
Cuts at: 0 1207 2016 9840 13689
Size: 1207 809 7824 3849
Fragments arranged by size:
7824 3849 1207 809
SspI AAT'ATT
Cuts at: 0 4361 6238 6309 6600 6705 7702 10008 10525
Size: 4361 1877 71 291 105 997 2306 517
Cuts at: 10525 11398 13162 13198 13341 13689
Size: 873 1764 36 143 348
Fragments arranged by size:
4361 2306 1877 1764 997 873 517 348
291 143 105 71 36
StuI AGG'CCT
Cuts at: 0 1070 3018 13689
Size: 1070 1948 10671
Fragments arranged by size:
10671 1948 1070
StyI C'CwwG_G
Cuts at: 0 1142 1493 1744 2573 3083 4581 4863 6413
Size: 1142 351 251 829 510 1498 282 1550
Cuts at: 6413 7418 7779 9855 9998 10370 10777 10845 12224
Size: 1005 361 2076 143 372 407 68 1379
Cuts at: 12224 13689
Size: 1465
Fragments arranged by size:
2076 1550 1498 1465 1379 1142 1005 829
510 407 372 361 351 282 251 143
68
SwaI ATTT'AAAT
Cuts at: 0 9074 13689
Size: 9074 4615
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2392 4048 5146 7577 13689
Size: 2392 1656 1098 2431 6112
Fragments arranged by size:
6112 2431 2392 1656 1098
TatI w'GTAC_w
Cuts at: 0 152 737 1546 2156 4097 6270 6730 9290
Size: 152 585 809 610 1941 2173 460 2560
Cuts at: 9290 9695 10133 10234 10406 11126 13689
Size: 405 438 101 172 720 2563
Fragments arranged by size:
2563 2560 2173 1941 809 720 610 585
460 438 405 172 152 101
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 936 968 1550 1582 3664 3696 3944 3976
Size: 936 32 582 32 2082 32 248 32
Cuts at: 3976 7960 7992 9417 9449 9535 9567 13689
Size: 3984 32 1425 32 86 32 4122
Fragments arranged by size:
4122 3984 2082 1425 936 582 248 86
32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2140 3177 13689
Size: 2140 1037 10512
Fragments arranged by size:
10512 2140 1037
XbaI T'CTAG_A
Cuts at: 0 4065 4290 7545 13689
Size: 4065 225 3255 6144
Fragments arranged by size:
6144 4065 3255 225
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3003 3579 6841 10257 13689
Size: 3003 576 3262 3416 3432
Fragments arranged by size:
3432 3416 3262 3003 576
XhoI C'TCGA_G
Cuts at: 0 3995 13689
Size: 3995 9694
XmnI GAAnn'nnTTC
Cuts at: 0 1418 3778 4249 6382 9445 9478 11897 13085
Size: 1418 2360 471 2133 3063 33 2419 1188
Cuts at: 13085 13689
Size: 604
Fragments arranged by size:
3063 2419 2360 2133 1418 1188 604 471
33
ZraI GAC'GTC
Cuts at: 0 9867 13689
Size: 9867 3822
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AleI AlfI AloI
AlwNI ApoI AseI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI MfeI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmlI PpiI PpuMI PsiI PsrI
PstI PvuII SacI SalI SanDI SapI ScaI SexAI
SfcI SfoI SmlI SnaBI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AhdI ApaI ApaLI
AscI AsiSI BaeI BmtI BsiEI BsiWI BsmBI BsrBI
BssHII BstZ17I EagI FseI FspAI KpnI MluI NheI
NotI NruI PacI PmeI PshAI PspOMI PspXI PvuI
RsrII SacII SbfI SfiI SgfI SgrAI SmaI SpeI
SrfI XmaI