Restriction map for
pF1KE0700
(Linear) (Six-base) MAPSORT of: KIEE0700.seq Check: 2790 from: 1 to: 5070
KIEE0700.orf
With 182 enzymes: SgfI *
March 12, 2010 15:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 204 5070
Size: 204 4866
AccI GT'mk_AC
Cuts at: 0 3410 5070
Size: 3410 1660
Acc65I G'GTAC_C
Cuts at: 0 2147 4739 5070
Size: 2147 2592 331
Fragments arranged by size:
2592 2147 331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 1135 1330 3133 4230 5070
Size: 132 1003 195 1803 1097 840
Fragments arranged by size:
1803 1097 1003 840 195 132
AflIII A'CryG_T
Cuts at: 0 200 269 410 4763 4814 5070
Size: 200 69 141 4353 51 256
Fragments arranged by size:
4353 256 200 141 69 51
AhdI GACnn_n'nnGTC
Cuts at: 0 411 641 4260 5070
Size: 411 230 3619 810
Fragments arranged by size:
3619 810 411 230
AleI CACnn'nnGTG
Cuts at: 0 1549 2350 4051 5070
Size: 1549 801 1701 1019
Fragments arranged by size:
1701 1549 1019 801
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 314 3289 4972 5070
Size: 314 2975 1683 98
Fragments arranged by size:
2975 1683 314 98
AlwNI CAG_nnn'CTG
Cuts at: 0 1932 1992 2424 4683 5070
Size: 1932 60 432 2259 387
Fragments arranged by size:
2259 1932 432 387 60
ApaI G_GGCC'C
Cuts at: 0 527 2864 2957 5070
Size: 527 2337 93 2113
Fragments arranged by size:
2337 2113 527 93
ApaLI G'TGCA_C
Cuts at: 0 4932 5070
Size: 4932 138
AvaI C'yCGr_G
Cuts at: 0 50 1281 1789 2077 2799 3311 4603 4649
Size: 50 1231 508 288 722 512 1292 46
Cuts at: 4649 5070
Size: 421
Fragments arranged by size:
1292 1231 722 512 508 421 288 50
46
BanI G'GyrC_C
Cuts at: 0 298 1441 2147 2463 4028 4549 4739 4799
Size: 298 1143 706 316 1565 521 190 60
Cuts at: 4799 5070
Size: 271
Fragments arranged by size:
1565 1143 706 521 316 298 271 190
60
BanII G_rGCy'C
Cuts at: 0 527 1207 1340 1676 1724 2078 2864 2957
Size: 527 680 133 336 48 354 786 93
Cuts at: 2957 3110 3980 4604 4676 5070
Size: 153 870 624 72 394
Fragments arranged by size:
870 786 680 624 527 394 354 336
153 133 93 72 48
BbeI G_GCGC'C
Cuts at: 0 4032 4803 5070
Size: 4032 771 267
Fragments arranged by size:
4032 771 267
BbsI GAAGACnn'nnnn_
Cuts at: 0 651 904 1617 3861 4174 5070
Size: 651 253 713 2244 313 896
Fragments arranged by size:
2244 896 713 651 313 253
BbvCI CC'TCA_GC
Cuts at: 0 1033 2865 4301 5070
Size: 1033 1832 1436 769
Fragments arranged by size:
1832 1436 1033 769
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2437 2471 2541 2575 5070
Size: 2437 34 70 34 2495
Fragments arranged by size:
2495 2437 70 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 256 3670 5070
Size: 256 3414 1400
Fragments arranged by size:
3414 1400 256
BfrBI ATG'CAT
Cuts at: 0 3488 4983 5070
Size: 3488 1495 87
Fragments arranged by size:
3488 1495 87
BglI GCCn_nnn'nGGC
Cuts at: 0 1405 2662 5070
Size: 1405 1257 2408
Fragments arranged by size:
2408 1405 1257
BglII A'GATC_T
Cuts at: 0 3419 5070
Size: 3419 1651
BlpI GC'TnA_GC
Cuts at: 0 1334 1841 3277 3352 3979 4144 5070
Size: 1334 507 1436 75 627 165 926
Fragments arranged by size:
1436 1334 926 627 507 165 75
Bme1580I G_kGCm'C
Cuts at: 0 527 787 1601 2468 2864 2906 2957 3341
Size: 527 260 814 867 396 42 51 384
Cuts at: 3341 4936 5070
Size: 1595 134
Fragments arranged by size:
1595 867 814 527 396 384 260 134
51 42
BmgBI CAC'GTC
Cuts at: 0 2159 2856 3153 3273 4413 4766 5070
Size: 2159 697 297 120 1140 353 304
Fragments arranged by size:
2159 1140 697 353 304 297 120
BmrI ACTGGGnnnn_n'
Cuts at: 0 1863 5070
Size: 1863 3207
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 558 2484 3114 4641 5070
Size: 558 1926 630 1527 429
Fragments arranged by size:
1926 1527 630 558 429
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1623 1900 1909 1954 1999 2022 2032 2425
Size: 1623 277 9 45 45 23 10 393
Cuts at: 2425 2454 3451 3599 4110 4156 4222 4260 4342
Size: 29 997 148 511 46 66 38 82
Cuts at: 4342 4774 5025 5070
Size: 432 251 45
Fragments arranged by size:
1623 997 511 432 393 277 251 148
82 66 46 45 45 45 38 29
23 10 9
Bpu10I CC'TnA_GC
Cuts at: 0 1024 1033 2413 2569 2865 4301 5070
Size: 1024 9 1380 156 296 1436 769
Fragments arranged by size:
1436 1380 1024 769 296 156 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 951 1011 2182 4891 5070
Size: 778 173 60 1171 2709 179
Fragments arranged by size:
2709 1171 778 179 173 60
BsaI GGTCTCn'nnnn_
Cuts at: 0 628 691 1012 1339 2504 3613 4198 5070
Size: 628 63 321 327 1165 1109 585 872
Fragments arranged by size:
1165 1109 872 628 585 327 321 63
BsaAI yAC'GTr
Cuts at: 0 531 3993 5070
Size: 531 3462 1077
Fragments arranged by size:
3462 1077 531
BsaHI Gr'CG_yC
Cuts at: 0 136 1834 2705 3025 4029 4800 5070
Size: 136 1698 871 320 1004 771 270
Fragments arranged by size:
1698 1004 871 771 320 270 136
BsaWI w'CCGG_w
Cuts at: 0 868 1382 1628 1775 2132 3673 3701 5070
Size: 868 514 246 147 357 1541 28 1369
Fragments arranged by size:
1541 1369 868 514 357 246 147 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1927 1957 4092 4122 4219 4249 5070
Size: 1927 30 2135 30 97 30 821
Fragments arranged by size:
2135 1927 821 97 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 931 1306 1369 1627 1975 2077 2080
Size: 250 681 375 63 258 348 102 3
Cuts at: 2080 2083 2086 2908 3493 4087 4263 4275 4503
Size: 3 3 822 585 594 176 12 228
Cuts at: 4503 5070
Size: 567
Fragments arranged by size:
822 681 594 585 567 375 348 258
250 228 176 102 63 12 3 3
3
BseYI C'CCAG_C
Cuts at: 0 519 748 1723 1865 3687 3833 4211 4676
Size: 519 229 975 142 1822 146 378 465
Cuts at: 4676 4772 5070
Size: 96 298
Fragments arranged by size:
1822 975 519 465 378 298 229 146
142 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2870 3813 4729 5070
Size: 2870 943 916 341
Fragments arranged by size:
2870 943 916 341
BsiEI CG_ry'CG
Cuts at: 0 2451 3458 4724 5070
Size: 2451 1007 1266 346
Fragments arranged by size:
2451 1266 1007 346
BsiHKAI G_wGCw'C
Cuts at: 0 512 1340 1619 3278 3980 4856 4936 5070
Size: 512 828 279 1659 702 876 80 134
Fragments arranged by size:
1659 876 828 702 512 279 134 80
BsmBI CGTCTCn'nnnn_
Cuts at: 0 127 1825 2165 2320 5070
Size: 127 1698 340 155 2750
Fragments arranged by size:
2750 1698 340 155 127
Bsp1286I G_dGCh'C
Cuts at: 0 512 527 787 1207 1340 1601 1619 1676
Size: 512 15 260 420 133 261 18 57
Cuts at: 1676 1724 2078 2468 2864 2906 2957 3110 3278
Size: 48 354 390 396 42 51 153 168
Cuts at: 3278 3341 3980 4604 4676 4856 4936 5070
Size: 63 639 624 72 180 80 134
Fragments arranged by size:
639 624 512 420 396 390 354 261
260 180 168 153 134 133 80 72
63 57 51 48 42 18 15
BspEI T'CCGG_A
Cuts at: 0 868 1382 1628 2132 3673 5070
Size: 868 514 246 504 1541 1397
Fragments arranged by size:
1541 1397 868 514 504 246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 204 1982 2180 2303 2585 3410 4190 4751
Size: 204 1778 198 123 282 825 780 561
Cuts at: 4751 5070
Size: 319
Fragments arranged by size:
1778 825 780 561 319 282 204 198
123
BsrBI CCG'CTC
Cuts at: 0 759 1956 2103 2798 4293 4845 5070
Size: 759 1197 147 695 1495 552 225
Fragments arranged by size:
1495 1197 759 695 552 225 147
BsrDI GCAATG_nn'
Cuts at: 0 1074 5070
Size: 1074 3996
BsrFI r'CCGG_y
Cuts at: 0 301 2503 3837 4025 5070
Size: 301 2202 1334 188 1045
Fragments arranged by size:
2202 1334 1045 301 188
BsrGI T'GTAC_A
Cuts at: 0 4817 5070
Size: 4817 253
BssSI C'ACGA_G
Cuts at: 0 1261 5070
Size: 1261 3809
BstAPI GCAn_nnn'nTGC
Cuts at: 0 516 3485 4182 4902 5070
Size: 516 2969 697 720 168
Fragments arranged by size:
2969 720 697 516 168
BstEII G'GTnAC_C
Cuts at: 0 1125 1662 2499 3174 3768 5070
Size: 1125 537 837 675 594 1302
Fragments arranged by size:
1302 1125 837 675 594 537
BstXI CCAn_nnnn'nTGG
Cuts at: 0 612 1162 1191 1386 2220 5070
Size: 612 550 29 195 834 2850
Fragments arranged by size:
2850 834 612 550 195 29
BstYI r'GATC_y
Cuts at: 0 1058 3419 4706 5070
Size: 1058 2361 1287 364
Fragments arranged by size:
2361 1287 1058 364
Bsu36I CC'TnA_GG
Cuts at: 0 185 3286 4561 5070
Size: 185 3101 1275 509
Fragments arranged by size:
3101 1275 509 185
BtgI C'CryG_G
Cuts at: 0 422 611 710 770 2357 2780 3458 3471
Size: 422 189 99 60 1587 423 678 13
Cuts at: 3471 4266 4838 4944 5070
Size: 795 572 106 126
Fragments arranged by size:
1587 795 678 572 423 422 189 126
106 99 60 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3147 5070
Size: 3147 1923
BtsI GCAGTG_nn'
Cuts at: 0 514 1617 2906 3173 3387 3933 4865 5070
Size: 514 1103 1289 267 214 546 932 205
Fragments arranged by size:
1289 1103 932 546 514 267 214 205
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 90 125 5070
Size: 90 35 4945
Fragments arranged by size:
4945 90 35
DraIII CAC_nnn'GTG
Cuts at: 0 2607 5070
Size: 2607 2463
DrdI GACnn_nn'nnGTC
Cuts at: 0 1696 3406 3627 5028 5070
Size: 1696 1710 221 1401 42
Fragments arranged by size:
1710 1696 1401 221 42
EaeI y'GGCC_r
Cuts at: 0 2 617 1406 1421 2354 2654 3231 3455
Size: 2 615 789 15 933 300 577 224
Cuts at: 3455 4352 4496 4585 4862 5015 5070
Size: 897 144 89 277 153 55
Fragments arranged by size:
933 897 789 615 577 300 277 224
153 144 89 55 15 2
EagI C'GGCC_G
Cuts at: 0 3455 5070
Size: 3455 1615
EarI CTCTTCn'nnn_
Cuts at: 0 382 2675 2810 3675 5070
Size: 382 2293 135 865 1395
Fragments arranged by size:
2293 1395 865 382 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 837 1383 2522 2698 2971 5070
Size: 25 812 546 1139 176 273 2099
Fragments arranged by size:
2099 1139 812 546 273 176 25
EcoICRI GAG'CTC
Cuts at: 0 1338 3978 5070
Size: 1338 2640 1092
Fragments arranged by size:
2640 1338 1092
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 1135 1330 1623 1900 1909 1954 1999
Size: 132 1003 195 293 277 9 45 45
Cuts at: 1999 2022 2032 2425 2454 3133 3451 3599 4110
Size: 23 10 393 29 679 318 148 511
Cuts at: 4110 4156 4222 4230 4260 4342 4774 5025 5070
Size: 46 66 8 30 82 432 251 45
Fragments arranged by size:
1003 679 511 432 393 318 293 277
251 195 148 132 82 66 46 45
45 45 30 29 23 10 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 796 835 1492 2200 3265 3508 4153 4156
Size: 796 39 657 708 1065 243 645 3
Cuts at: 4156 4957 5070
Size: 801 113
Fragments arranged by size:
1065 801 796 708 657 645 243 113
39 3
EcoO109I rG'GnC_Cy
Cuts at: 0 372 523 524 537 579 1086 1140 1497
Size: 372 151 1 13 42 507 54 357
Cuts at: 1497 2169 2555 2685 2860 2861 2954 3130 3290
Size: 672 386 130 175 1 93 176 160
Cuts at: 3290 3379 3494 4094 4470 4565 5070
Size: 89 115 600 376 95 505
Fragments arranged by size:
672 600 507 505 386 376 372 357
176 175 160 151 130 115 95 93
89 54 42 13 1 1
HaeII r_GCGC'y
Cuts at: 0 687 2122 4026 4032 4283 4683 4785 4803
Size: 687 1435 1904 6 251 400 102 18
Cuts at: 4803 5070
Size: 267
Fragments arranged by size:
1904 1435 687 400 267 251 102 18
6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 364 396 397 429 627 659 2683 2715
Size: 364 32 1 32 198 32 2024 32
Cuts at: 2715 3773 3805 4092 4124 4219 4246 4251 4278
Size: 1058 32 287 32 95 27 5 27
Cuts at: 4278 4470 4502 5070
Size: 192 32 568
Fragments arranged by size:
2024 1058 568 364 287 198 192 95
32 32 32 32 32 32 32 27
27 5 1
HincII GTy'rAC
Cuts at: 0 114 932 1485 2880 5070
Size: 114 818 553 1395 2190
Fragments arranged by size:
2190 1395 818 553 114
HindIII A'AGCT_T
Cuts at: 0 144 5070
Size: 144 4926
HpaI GTT'AAC
Cuts at: 0 114 5070
Size: 114 4956
KasI G'GCGC_C
Cuts at: 0 4028 4799 5070
Size: 4028 771 271
Fragments arranged by size:
4028 771 271
KpnI G_GTAC'C
Cuts at: 0 2151 4743 5070
Size: 2151 2592 327
Fragments arranged by size:
2592 2151 327
MluI A'CGCG_T
Cuts at: 0 200 5070
Size: 200 4870
MscI TGG'CCA
Cuts at: 0 1423 3233 4498 4864 5070
Size: 1423 1810 1265 366 206
Fragments arranged by size:
1810 1423 1265 366 206
MslI CAynn'nnrTG
Cuts at: 0 85 319 625 1177 1384 1549 1753 1897
Size: 85 234 306 552 207 165 204 144
Cuts at: 1897 2218 2350 3166 4051 5070
Size: 321 132 816 885 1019
Fragments arranged by size:
1019 885 816 552 321 306 234 207
204 165 144 132 85
MspA1I CmG'CkG
Cuts at: 0 1064 1503 1840 1989 3569 4215 4344 4946
Size: 1064 439 337 149 1580 646 129 602
Cuts at: 4946 5070
Size: 124
Fragments arranged by size:
1580 1064 646 602 439 337 149 129
124
NaeI GCC'GGC
Cuts at: 0 303 3839 4027 5070
Size: 303 3536 188 1043
Fragments arranged by size:
3536 1043 303 188
NarI GG'CG_CC
Cuts at: 0 4029 4800 5070
Size: 4029 771 270
Fragments arranged by size:
4029 771 270
NcoI C'CATG_G
Cuts at: 0 4266 5070
Size: 4266 804
NgoMIV G'CCGG_C
Cuts at: 0 301 3837 4025 5070
Size: 301 3536 188 1045
Fragments arranged by size:
3536 1045 301 188
NsiI A_TGCA'T
Cuts at: 0 3490 4985 5070
Size: 3490 1495 85
Fragments arranged by size:
3490 1495 85
NspI r_CATG'y
Cuts at: 0 253 414 3483 4818 4983 5070
Size: 253 161 3069 1335 165 87
Fragments arranged by size:
3069 1335 253 165 161 87
PasI CC'CwG_GG
Cuts at: 0 831 1155 4012 5070
Size: 831 324 2857 1058
Fragments arranged by size:
2857 1058 831 324
PciI A'CATG_T
Cuts at: 0 410 4814 5070
Size: 410 4404 256
Fragments arranged by size:
4404 410 256
PflMI CCAn_nnn'nTGG
Cuts at: 0 1932 5070
Size: 1932 3138
PfoI T'CCnGG_A
Cuts at: 0 575 944 1925 2366 3375 4040 4223 4466
Size: 575 369 981 441 1009 665 183 243
Cuts at: 4466 4700 4751 5070
Size: 234 51 319
Fragments arranged by size:
1009 981 665 575 441 369 319 243
234 183 51
PmlI CAC'GTG
Cuts at: 0 3993 5070
Size: 3993 1077
PpuMI rG'GwC_Cy
Cuts at: 0 372 537 579 1086 1140 1497 2169 3379
Size: 372 165 42 507 54 357 672 1210
Cuts at: 3379 3494 4470 4565 5070
Size: 115 976 95 505
Fragments arranged by size:
1210 976 672 507 505 372 357 165
115 95 54 42
PspOMI G'GGCC_C
Cuts at: 0 523 2860 2953 5070
Size: 523 2337 93 2117
Fragments arranged by size:
2337 2117 523 93
PstI C_TGCA'G
Cuts at: 0 248 1002 2024 2460 3023 4157 4961 5070
Size: 248 754 1022 436 563 1134 804 109
Fragments arranged by size:
1134 1022 804 754 563 436 248 109
PvuI CG_AT'CG
Cuts at: 0 4724 5070
Size: 4724 346
PvuII CAG'CTG
Cuts at: 0 1840 5070
Size: 1840 3230
SacI G_AGCT'C
Cuts at: 0 1340 3980 5070
Size: 1340 2640 1090
Fragments arranged by size:
2640 1340 1090
SacII CC_GC'GG
Cuts at: 0 4947 5070
Size: 4947 123
SanDI GG'GwC_CC
Cuts at: 0 3379 4470 5070
Size: 3379 1091 600
Fragments arranged by size:
3379 1091 600
SapI GCTCTTCn'nnn_
Cuts at: 0 2810 5070
Size: 2810 2260
SbfI CC_TGCA'GG
Cuts at: 0 3023 4157 5070
Size: 3023 1134 913
Fragments arranged by size:
3023 1134 913
SfcI C'TryA_G
Cuts at: 0 244 998 2020 2456 3019 4153 4957 5070
Size: 244 754 1022 436 563 1134 804 113
Fragments arranged by size:
1134 1022 804 754 563 436 244 113
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2662 5070
Size: 2662 2408
SfoI GGC'GCC
Cuts at: 0 4030 4801 5070
Size: 4030 771 269
Fragments arranged by size:
4030 771 269
SgrAI Cr'CCGG_yG
Cuts at: 0 4025 5070
Size: 4025 1045
SmaI CCC'GGG
Cuts at: 0 52 1283 4651 5070
Size: 52 1231 3368 419
Fragments arranged by size:
3368 1231 419 52
SmlI C'TyrA_G
Cuts at: 0 793 966 990 2197 4906 5070
Size: 793 173 24 1207 2709 164
Fragments arranged by size:
2709 1207 793 173 164 24
SphI G_CATG'C
Cuts at: 0 253 3483 5070
Size: 253 3230 1587
Fragments arranged by size:
3230 1587 253
SrfI GCCC'GGGC
Cuts at: 0 4651 5070
Size: 4651 419
StuI AGG'CCT
Cuts at: 0 2915 3094 5070
Size: 2915 179 1976
Fragments arranged by size:
2915 1976 179
StyI C'CwwG_G
Cuts at: 0 128 779 1445 1667 3851 4266 5070
Size: 128 651 666 222 2184 415 804
Fragments arranged by size:
2184 804 666 651 415 222 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 527 691 1405 2336 2404 3637 3701 3847
Size: 527 164 714 931 68 1233 64 146
Cuts at: 3847 4929 5070
Size: 1082 141
Fragments arranged by size:
1233 1082 931 714 527 164 146 141
68 64
TatI w'GTAC_w
Cuts at: 0 3194 4397 4817 4880 5070
Size: 3194 1203 420 63 190
Fragments arranged by size:
3194 1203 420 190 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 254 286 2161 2193 4111 4143 5070
Size: 254 32 1875 32 1918 32 927
Fragments arranged by size:
1918 1875 927 254 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3180 5070
Size: 3180 1890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1191 1974 2355 4785 4872 5070
Size: 1191 783 381 2430 87 198
Fragments arranged by size:
2430 1191 783 381 198 87
XmaI C'CCGG_G
Cuts at: 0 50 1281 4649 5070
Size: 50 1231 3368 421
Fragments arranged by size:
3368 1231 421 50
XmnI GAAnn'nnTTC
Cuts at: 0 2641 2819 5070
Size: 2641 178 2251
Fragments arranged by size:
2641 2251 178
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuI PvuII SacI
SacII SanDI SapI SbfI SfcI SfiI SfoI SgrAI
SmaI SmlI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AloI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BclI BmtI BsaBI
BsiWI BsmI BspHI BssHII BstBI BstZ17I ClaI DraI
EcoRI EcoRV FalI FseI FspI FspAI MfeI MmeI
NdeI NheI NotI NruI PacI PmeI PpiI PshAI
PsiI PspXI PsrI RsrII SalI ScaI SexAI SgfI
SnaBI SpeI SspI SwaI XbaI XhoI ZraI