(Linear) (Six-base) MAPSORT of: KIEE0702.seq Check: 7132 from: 1 to: 1218 KIEE0702.orf With 182 enzymes: SgfI * April 24, 2009 13:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 807 1218 Size: 807 411 AatII G_ACGT'C Cuts at: 0 86 1218 Size: 86 1132 AccI GT'mk_AC Cuts at: 0 641 1218 Size: 641 577 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 481 1181 1218 Size: 481 700 37 Fragments arranged by size: 700 481 37 AlwNI CAG_nnn'CTG Cuts at: 0 717 1218 Size: 717 501 ApaI G_GGCC'C Cuts at: 0 1159 1218 Size: 1159 59 AvaI C'yCGr_G Cuts at: 0 466 763 1218 Size: 466 297 455 Fragments arranged by size: 466 455 297 BamHI G'GATC_C Cuts at: 0 822 1218 Size: 822 396 BanI G'GyrC_C Cuts at: 0 701 1040 1218 Size: 701 339 178 Fragments arranged by size: 701 339 178 BanII G_rGCy'C Cuts at: 0 106 1159 1218 Size: 106 1053 59 Fragments arranged by size: 1053 106 59 BbsI GAAGACnn'nnnn_ Cuts at: 0 287 1218 Size: 287 931 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 621 655 1039 1073 1218 Size: 621 34 384 34 145 Fragments arranged by size: 621 384 145 34 34 BclI T'GATC_A Cuts at: 0 731 1218 Size: 731 487 BglI GCCn_nnn'nGGC Cuts at: 0 1137 1218 Size: 1137 81 BglII A'GATC_T Cuts at: 0 456 1218 Size: 456 762 BlpI GC'TnA_GC Cuts at: 0 176 845 1218 Size: 176 669 373 Fragments arranged by size: 669 373 176 Bme1580I G_kGCm'C Cuts at: 0 1079 1159 1218 Size: 1079 80 59 Fragments arranged by size: 1079 80 59 BmgBI CAC'GTC Cuts at: 0 12 1218 Size: 12 1206 BmrI ACTGGGnnnn_n' Cuts at: 0 48 1089 1218 Size: 48 1041 129 Fragments arranged by size: 1041 129 48 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1191 1218 Size: 1191 27 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 789 1218 Size: 220 569 429 Fragments arranged by size: 569 429 220 Bpu10I CC'TnA_GC Cuts at: 0 191 967 1218 Size: 191 776 251 Fragments arranged by size: 776 251 191 BsaI GGTCTCn'nnnn_ Cuts at: 0 410 1218 Size: 410 808 BsaHI Gr'CG_yC Cuts at: 0 83 1218 Size: 83 1135 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 730 760 787 817 1218 Size: 730 30 27 30 401 Fragments arranged by size: 730 401 30 30 27 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 110 217 1180 1218 Size: 110 107 963 38 Fragments arranged by size: 963 110 107 38 BseYI C'CCAG_C Cuts at: 0 1212 1218 Size: 1212 6 BsiHKAI G_wGCw'C Cuts at: 0 106 1218 Size: 106 1112 BsmI GAATG_Cn' Cuts at: 0 1005 1218 Size: 1005 213 Bsp1286I G_dGCh'C Cuts at: 0 106 1079 1159 1218 Size: 106 973 80 59 Fragments arranged by size: 973 106 80 59 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 807 1218 Size: 807 411 BsrBI CCG'CTC Cuts at: 0 180 1218 Size: 180 1038 BsrFI r'CCGG_y Cuts at: 0 253 704 1127 1218 Size: 253 451 423 91 Fragments arranged by size: 451 423 253 91 BssSI C'ACGA_G Cuts at: 0 142 1218 Size: 142 1076 BstXI CCAn_nnnn'nTGG Cuts at: 0 698 1218 Size: 698 520 BstYI r'GATC_y Cuts at: 0 456 497 590 713 822 1218 Size: 456 41 93 123 109 396 Fragments arranged by size: 456 396 123 109 93 41 Bsu36I CC'TnA_GG Cuts at: 0 77 827 1218 Size: 77 750 391 Fragments arranged by size: 750 391 77 BtgI C'CryG_G Cuts at: 0 605 1218 Size: 605 613 BtsI GCAGTG_nn' Cuts at: 0 425 938 1218 Size: 425 513 280 Fragments arranged by size: 513 425 280 ClaI AT'CG_AT Cuts at: 0 281 1218 Size: 281 937 EaeI y'GGCC_r Cuts at: 0 138 255 985 1218 Size: 138 117 730 233 Fragments arranged by size: 730 233 138 117 EarI CTCTTCn'nnn_ Cuts at: 0 1157 1218 Size: 1157 61 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 103 1218 Size: 103 1115 EcoICRI GAG'CTC Cuts at: 0 104 1218 Size: 104 1114 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 481 789 1181 1218 Size: 220 261 308 392 37 Fragments arranged by size: 392 308 261 220 37 EcoNI CCTnn'n_nnAGG Cuts at: 0 244 1218 Size: 244 974 EcoO109I rG'GnC_Cy Cuts at: 0 566 852 1014 1156 1218 Size: 566 286 162 142 62 Fragments arranged by size: 566 286 162 142 62 HaeII r_GCGC'y Cuts at: 0 586 1218 Size: 586 632 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 787 819 1218 Size: 787 32 399 Fragments arranged by size: 787 399 32 HincII GTy'rAC Cuts at: 0 642 1218 Size: 642 576 HindIII A'AGCT_T Cuts at: 0 547 1218 Size: 547 671 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 525 1218 Size: 525 693 MscI TGG'CCA Cuts at: 0 140 1218 Size: 140 1078 MslI CAynn'nnrTG Cuts at: 0 733 1218 Size: 733 485 MspA1I CmG'CkG Cuts at: 0 175 198 844 918 1218 Size: 175 23 646 74 300 Fragments arranged by size: 646 300 175 74 23 NaeI GCC'GGC Cuts at: 0 255 1218 Size: 255 963 NcoI C'CATG_G Cuts at: 0 605 1218 Size: 605 613 NgoMIV G'CCGG_C Cuts at: 0 253 1218 Size: 253 965 PasI CC'CwG_GG Cuts at: 0 571 1135 1218 Size: 571 564 83 Fragments arranged by size: 571 564 83 PflMI CCAn_nnn'nTGG Cuts at: 0 628 1218 Size: 628 590 PpuMI rG'GwC_Cy Cuts at: 0 566 1014 1218 Size: 566 448 204 Fragments arranged by size: 566 448 204 PspOMI G'GGCC_C Cuts at: 0 1155 1218 Size: 1155 63 PstI C_TGCA'G Cuts at: 0 818 1218 Size: 818 400 PvuII CAG'CTG Cuts at: 0 175 198 844 1218 Size: 175 23 646 374 Fragments arranged by size: 646 374 175 23 SacI G_AGCT'C Cuts at: 0 106 1218 Size: 106 1112 SalI G'TCGA_C Cuts at: 0 640 1218 Size: 640 578 SanDI GG'GwC_CC Cuts at: 0 566 1014 1218 Size: 566 448 204 Fragments arranged by size: 566 448 204 SexAI A'CCwGG_T Cuts at: 0 388 1218 Size: 388 830 SfcI C'TryA_G Cuts at: 0 814 856 1218 Size: 814 42 362 Fragments arranged by size: 814 362 42 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1137 1218 Size: 1137 81 SgrAI Cr'CCGG_yG Cuts at: 0 704 1218 Size: 704 514 SspI AAT'ATT Cuts at: 0 474 1218 Size: 474 744 StyI C'CwwG_G Cuts at: 0 605 1218 Size: 605 613 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 635 988 1218 Size: 635 353 230 Fragments arranged by size: 635 353 230 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 535 625 1218 Size: 535 90 593 Fragments arranged by size: 593 535 90 ZraI GAC'GTC Cuts at: 0 84 1218 Size: 84 1134 Enzymes that do cut: AarI AatII AccI AcuI AlwNI ApaI AvaI BamHI BanI BanII BbsI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BssSI BstXI BstYI Bsu36I BtgI BtsI ClaI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PpuMI PspOMI PstI PvuII SacI SalI SanDI SexAI SfcI SfiI SgrAI SspI StyI TaqII XcmI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BciVI BfrBI BmtI BpuEI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgZI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SapI SbfI ScaI SfoI SgfI SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI