Restriction map for pF1KE0702
 (Linear) (Six-base) MAPSORT of: KIEE0702.seq  Check: 7132  from: 1  to: 1218



KIEE0702.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     807    1218 
   Size:        807     411
 
AatII G_ACGT'C

Cuts at:      0      86    1218 
   Size:         86    1132
 
AccI GT'mk_AC

Cuts at:      0     641    1218 
   Size:        641     577
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     481    1181    1218 
   Size:        481     700      37

  Fragments arranged by size: 

                 700     481      37
 
AlwNI CAG_nnn'CTG

Cuts at:      0     717    1218 
   Size:        717     501
 
ApaI G_GGCC'C

Cuts at:      0    1159    1218 
   Size:       1159      59
 
AvaI C'yCGr_G

Cuts at:      0     466     763    1218 
   Size:        466     297     455

  Fragments arranged by size: 

                 466     455     297
 
BamHI G'GATC_C

Cuts at:      0     822    1218 
   Size:        822     396
 
BanI G'GyrC_C

Cuts at:      0     701    1040    1218 
   Size:        701     339     178

  Fragments arranged by size: 

                 701     339     178
 
BanII G_rGCy'C

Cuts at:      0     106    1159    1218 
   Size:        106    1053      59

  Fragments arranged by size: 

                1053     106      59
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     287    1218 
   Size:        287     931
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     621     655    1039    1073    1218 
   Size:        621      34     384      34     145

  Fragments arranged by size: 

                 621     384     145      34      34
 
BclI T'GATC_A

Cuts at:      0     731    1218 
   Size:        731     487
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1137    1218 
   Size:       1137      81
 
BglII A'GATC_T

Cuts at:      0     456    1218 
   Size:        456     762
 
BlpI GC'TnA_GC

Cuts at:      0     176     845    1218 
   Size:        176     669     373

  Fragments arranged by size: 

                 669     373     176
 
Bme1580I G_kGCm'C

Cuts at:      0    1079    1159    1218 
   Size:       1079      80      59

  Fragments arranged by size: 

                1079      80      59
 
BmgBI CAC'GTC

Cuts at:      0      12    1218 
   Size:         12    1206
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      48    1089    1218 
   Size:         48    1041     129

  Fragments arranged by size: 

                1041     129      48
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1191    1218 
   Size:       1191      27
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     789    1218 
   Size:        220     569     429

  Fragments arranged by size: 

                 569     429     220
 
Bpu10I CC'TnA_GC

Cuts at:      0     191     967    1218 
   Size:        191     776     251

  Fragments arranged by size: 

                 776     251     191
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     410    1218 
   Size:        410     808
 
BsaHI Gr'CG_yC

Cuts at:      0      83    1218 
   Size:         83    1135
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     730     760     787     817    1218 
   Size:        730      30      27      30     401

  Fragments arranged by size: 

                 730     401      30      30      27
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     110     217    1180    1218 
   Size:        110     107     963      38

  Fragments arranged by size: 

                 963     110     107      38
 
BseYI C'CCAG_C

Cuts at:      0    1212    1218 
   Size:       1212       6
 
BsiHKAI G_wGCw'C

Cuts at:      0     106    1218 
   Size:        106    1112
 
BsmI GAATG_Cn'

Cuts at:      0    1005    1218 
   Size:       1005     213
 
Bsp1286I G_dGCh'C

Cuts at:      0     106    1079    1159    1218 
   Size:        106     973      80      59

  Fragments arranged by size: 

                 973     106      80      59
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     807    1218 
   Size:        807     411
 
BsrBI CCG'CTC

Cuts at:      0     180    1218 
   Size:        180    1038
 
BsrFI r'CCGG_y

Cuts at:      0     253     704    1127    1218 
   Size:        253     451     423      91

  Fragments arranged by size: 

                 451     423     253      91
 
BssSI C'ACGA_G

Cuts at:      0     142    1218 
   Size:        142    1076
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     698    1218 
   Size:        698     520
 
BstYI r'GATC_y

Cuts at:      0     456     497     590     713     822    1218 
   Size:        456      41      93     123     109     396

  Fragments arranged by size: 

                 456     396     123     109      93      41
 
Bsu36I CC'TnA_GG

Cuts at:      0      77     827    1218 
   Size:         77     750     391

  Fragments arranged by size: 

                 750     391      77
 
BtgI C'CryG_G

Cuts at:      0     605    1218 
   Size:        605     613
 
BtsI GCAGTG_nn'

Cuts at:      0     425     938    1218 
   Size:        425     513     280

  Fragments arranged by size: 

                 513     425     280
 
ClaI AT'CG_AT

Cuts at:      0     281    1218 
   Size:        281     937
 
EaeI y'GGCC_r

Cuts at:      0     138     255     985    1218 
   Size:        138     117     730     233

  Fragments arranged by size: 

                 730     233     138     117
 
EarI CTCTTCn'nnn_

Cuts at:      0    1157    1218 
   Size:       1157      61
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     103    1218 
   Size:        103    1115
 
EcoICRI GAG'CTC

Cuts at:      0     104    1218 
   Size:        104    1114
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     481     789    1181    1218 
   Size:        220     261     308     392      37

  Fragments arranged by size: 

                 392     308     261     220      37
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     244    1218 
   Size:        244     974
 
EcoO109I rG'GnC_Cy

Cuts at:      0     566     852    1014    1156    1218 
   Size:        566     286     162     142      62

  Fragments arranged by size: 

                 566     286     162     142      62
 
HaeII r_GCGC'y

Cuts at:      0     586    1218 
   Size:        586     632
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     787     819    1218 
   Size:        787      32     399

  Fragments arranged by size: 

                 787     399      32
 
HincII GTy'rAC

Cuts at:      0     642    1218 
   Size:        642     576
 
HindIII A'AGCT_T

Cuts at:      0     547    1218 
   Size:        547     671
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     525    1218 
   Size:        525     693
 
MscI TGG'CCA

Cuts at:      0     140    1218 
   Size:        140    1078
 
MslI CAynn'nnrTG

Cuts at:      0     733    1218 
   Size:        733     485
 
MspA1I CmG'CkG

Cuts at:      0     175     198     844     918    1218 
   Size:        175      23     646      74     300

  Fragments arranged by size: 

                 646     300     175      74      23
 
NaeI GCC'GGC

Cuts at:      0     255    1218 
   Size:        255     963
 
NcoI C'CATG_G

Cuts at:      0     605    1218 
   Size:        605     613
 
NgoMIV G'CCGG_C

Cuts at:      0     253    1218 
   Size:        253     965
 
PasI CC'CwG_GG

Cuts at:      0     571    1135    1218 
   Size:        571     564      83

  Fragments arranged by size: 

                 571     564      83
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     628    1218 
   Size:        628     590
 
PpuMI rG'GwC_Cy

Cuts at:      0     566    1014    1218 
   Size:        566     448     204

  Fragments arranged by size: 

                 566     448     204
 
PspOMI G'GGCC_C

Cuts at:      0    1155    1218 
   Size:       1155      63
 
PstI C_TGCA'G

Cuts at:      0     818    1218 
   Size:        818     400
 
PvuII CAG'CTG

Cuts at:      0     175     198     844    1218 
   Size:        175      23     646     374

  Fragments arranged by size: 

                 646     374     175      23
 
SacI G_AGCT'C

Cuts at:      0     106    1218 
   Size:        106    1112
 
SalI G'TCGA_C

Cuts at:      0     640    1218 
   Size:        640     578
 
SanDI GG'GwC_CC

Cuts at:      0     566    1014    1218 
   Size:        566     448     204

  Fragments arranged by size: 

                 566     448     204
 
SexAI A'CCwGG_T

Cuts at:      0     388    1218 
   Size:        388     830
 
SfcI C'TryA_G

Cuts at:      0     814     856    1218 
   Size:        814      42     362

  Fragments arranged by size: 

                 814     362      42
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1137    1218 
   Size:       1137      81
 
SgrAI Cr'CCGG_yG

Cuts at:      0     704    1218 
   Size:        704     514
 
SspI AAT'ATT

Cuts at:      0     474    1218 
   Size:        474     744
 
StyI C'CwwG_G

Cuts at:      0     605    1218 
   Size:        605     613
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     635     988    1218 
   Size:        635     353     230

  Fragments arranged by size: 

                 635     353     230
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     535     625    1218 
   Size:        535      90     593

  Fragments arranged by size: 

                 593     535      90
 
ZraI GAC'GTC

Cuts at:      0      84    1218 
   Size:         84    1134
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI    AlwNI     ApaI     AvaI    BamHI
     BanI    BanII     BbsI     BcgI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaHI
    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI
    BsrFI    BssSI    BstXI    BstYI   Bsu36I     BtgI     BtsI     ClaI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII
    Hin4I   HincII  HindIII     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     PasI    PflMI    PpuMI   PspOMI     PstI    PvuII
     SacI     SalI    SanDI    SexAI     SfcI     SfiI    SgrAI     SspI
     StyI    TaqII     XcmI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI    BbvCI    BciVI    BfrBI     BmtI    BpuEI    BsaAI
    BsaBI    BsaWI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrDI    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I    BtgZI
    CspCI     DraI   DraIII     DrdI     EagI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI     HpaI     KasI     KpnI     MfeI     MluI
     NarI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SapI     SbfI     ScaI     SfoI
     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
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