Restriction map for
pF1KE0705
(Linear) (Six-base) MAPSORT of: KIEE0705.seq Check: 7072 from: 1 to: 885
KIEE0705.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 0 4 885
Size: 4 881
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 689 885
Size: 343 346 196
Fragments arranged by size:
346 343 196
AleI CACnn'nnGTG
Cuts at: 0 579 885
Size: 579 306
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 127 885
Size: 127 758
AlwNI CAG_nnn'CTG
Cuts at: 0 667 885
Size: 667 218
ApaI G_GGCC'C
Cuts at: 0 334 885
Size: 334 551
ApoI r'AATT_y
Cuts at: 0 303 351 516 885
Size: 303 48 165 369
Fragments arranged by size:
369 303 165 48
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 749 782 885
Size: 749 33 103
Fragments arranged by size:
749 103 33
BanII G_rGCy'C
Cuts at: 0 334 885
Size: 334 551
Bme1580I G_kGCm'C
Cuts at: 0 334 885
Size: 334 551
BmgBI CAC'GTC
Cuts at: 0 270 379 885
Size: 270 109 506
Fragments arranged by size:
506 270 109
BmrI ACTGGGnnnn_n'
Cuts at: 0 51 885
Size: 51 834
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 885
Size: 24 861
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 800 885
Size: 800 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 656 686 885
Size: 656 30 199
Fragments arranged by size:
656 199 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 392 885
Size: 392 493
BseYI C'CCAG_C
Cuts at: 0 327 885
Size: 327 558
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 391 885
Size: 298 93 494
Fragments arranged by size:
494 298 93
Bsp1286I G_dGCh'C
Cuts at: 0 334 885
Size: 334 551
BspHI T'CATG_A
Cuts at: 0 696 885
Size: 696 189
BsrDI GCAATG_nn'
Cuts at: 0 875 885
Size: 875 10
BstXI CCAn_nnnn'nTGG
Cuts at: 0 581 885
Size: 581 304
BtgI C'CryG_G
Cuts at: 0 309 885
Size: 309 576
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 365 400 885
Size: 365 35 485
Fragments arranged by size:
485 365 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 343 689 885
Size: 24 319 346 196
Fragments arranged by size:
346 319 196 24
EcoO109I rG'GnC_Cy
Cuts at: 0 331 885
Size: 331 554
EcoRV GAT'ATC
Cuts at: 0 544 885
Size: 544 341
HincII GTy'rAC
Cuts at: 0 636 885
Size: 636 249
HindIII A'AGCT_T
Cuts at: 0 171 885
Size: 171 714
HpaI GTT'AAC
Cuts at: 0 636 885
Size: 636 249
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 885
Size: 111 774
MslI CAynn'nnrTG
Cuts at: 0 579 647 885
Size: 579 68 238
Fragments arranged by size:
579 238 68
MspA1I CmG'CkG
Cuts at: 0 70 631 885
Size: 70 561 254
Fragments arranged by size:
561 254 70
NcoI C'CATG_G
Cuts at: 0 309 885
Size: 309 576
PflMI CCAn_nnn'nTGG
Cuts at: 0 715 885
Size: 715 170
PfoI T'CCnGG_A
Cuts at: 0 660 885
Size: 660 225
PspOMI G'GGCC_C
Cuts at: 0 330 885
Size: 330 555
PvuII CAG'CTG
Cuts at: 0 70 631 885
Size: 70 561 254
Fragments arranged by size:
561 254 70
SmlI C'TyrA_G
Cuts at: 0 815 885
Size: 815 70
StyI C'CwwG_G
Cuts at: 0 81 309 885
Size: 81 228 576
Fragments arranged by size:
576 228 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 70 761 885
Size: 70 691 124
Fragments arranged by size:
691 124 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 459 491 885
Size: 459 32 394
Fragments arranged by size:
459 394 32
Enzymes that do cut:
AccI AcuI AleI AlfI AlwNI ApaI ApoI BaeI
BanII Bme1580I BmgBI BmrI BpmI BpuEI BsaXI BseRI
BseYI BsgI Bsp1286I BspHI BsrDI BstXI BtgI CspCI
Eco57MI EcoO109I EcoRV HincII HindIII HpaI MmeI MslI
MspA1I NcoI PflMI PfoI PspOMI PvuII SmlI StyI
TaqII TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HaeII
Hin4I KasI KpnI MfeI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI