(Linear) (Six-base) MAPSORT of: KIEE0709.seq  Check: 6604  from: 1  to: 885



KIEE0709.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AccI GT'mk_AC

Cuts at:      0     215     885 
   Size:        215     670
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     515     885 
   Size:        322     193     370

  Fragments arranged by size: 

                 370     322     193
 
AflIII A'CryG_T

Cuts at:      0     380     885 
   Size:        380     505
 
ApoI r'AATT_y

Cuts at:      0     240     265     786     885 
   Size:        240      25     521      99

  Fragments arranged by size: 

                 521     240      99      25
 
AseI AT'TA_AT

Cuts at:      0      97     885 
   Size:         97     788
 
AvaI C'yCGr_G

Cuts at:      0     779     885 
   Size:        779     106
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     310     885 
   Size:        310     575
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     106     885 
   Size:        106     779
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     830     885 
   Size:        830      55
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     556     885 
   Size:        556     329
 
BsrDI GCAATG_nn'

Cuts at:      0     878     885 
   Size:        878       7
 
BstYI r'GATC_y

Cuts at:      0      38     885 
   Size:         38     847
 
Bsu36I CC'TnA_GG

Cuts at:      0     604     885 
   Size:        604     281
 
BtgI C'CryG_G

Cuts at:      0     470     885 
   Size:        470     415
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     515     556     885 
   Size:        322     193      41     329

  Fragments arranged by size: 

                 329     322     193      41
 
EcoRI G'AATT_C

Cuts at:      0     240     885 
   Size:        240     645
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      51      83     885 
   Size:         51      32     802

  Fragments arranged by size: 

                 802      51      32
 
HincII GTy'rAC

Cuts at:      0     198     885 
   Size:        198     687
 
MslI CAynn'nnrTG

Cuts at:      0     801     885 
   Size:        801      84
 
NspI r_CATG'y

Cuts at:      0     227     533     885 
   Size:        227     306     352

  Fragments arranged by size: 

                 352     306     227
 
PfoI T'CCnGG_A

Cuts at:      0      44     547     885 
   Size:         44     503     338

  Fragments arranged by size: 

                 503     338      44
 
PspXI vC'TCGA_Gb

Cuts at:      0     779     885 
   Size:        779     106
 
SmlI C'TyrA_G

Cuts at:      0     779     885 
   Size:        779     106
 
StuI AGG'CCT

Cuts at:      0     609     885 
   Size:        609     276
 
TatI w'GTAC_w

Cuts at:      0      77     294     510     885 
   Size:         77     217     216     375

  Fragments arranged by size: 

                 375     217     216      77
 
XhoI C'TCGA_G

Cuts at:      0     779     885 
   Size:        779     106
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     ApoI     AseI     AvaI     BbsI    BciVI
     BmrI     BpmI    BsrDI    BstYI   Bsu36I     BtgI  Eco57MI    EcoRI
    Hin4I   HincII     MslI     NspI     PfoI    PspXI     SmlI     StuI
     TatI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI     ZraI