Restriction map for
pF1KE0717
(Linear) (Six-base) MAPSORT of: KIEE0717.seq Check: 3557 from: 1 to: 915
KIEE0717.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 915
Size: 256 659
AfeI AGC'GCT
Cuts at: 0 136 915
Size: 136 779
AhdI GACnn_n'nnGTC
Cuts at: 0 399 915
Size: 399 516
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 610 642 915
Size: 610 32 273
Fragments arranged by size:
610 273 32
AlwNI CAG_nnn'CTG
Cuts at: 0 839 915
Size: 839 76
ApaI G_GGCC'C
Cuts at: 0 800 915
Size: 800 115
ApoI r'AATT_y
Cuts at: 0 6 915
Size: 6 909
AvaI C'yCGr_G
Cuts at: 0 251 572 915
Size: 251 321 343
Fragments arranged by size:
343 321 251
BanI G'GyrC_C
Cuts at: 0 274 364 915
Size: 274 90 551
Fragments arranged by size:
551 274 90
BanII G_rGCy'C
Cuts at: 0 800 915
Size: 800 115
BbvCI CC'TCA_GC
Cuts at: 0 329 915
Size: 329 586
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 370 404 915
Size: 370 34 511
Fragments arranged by size:
511 370 34
BglI GCCn_nnn'nGGC
Cuts at: 0 71 193 587 915
Size: 71 122 394 328
Fragments arranged by size:
394 328 122 71
Bme1580I G_kGCm'C
Cuts at: 0 279 800 915
Size: 279 521 115
Fragments arranged by size:
521 279 115
BmgBI CAC'GTC
Cuts at: 0 324 915
Size: 324 591
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 369 915
Size: 369 546
Bpu10I CC'TnA_GC
Cuts at: 0 329 915
Size: 329 586
BsaI GGTCTCn'nnnn_
Cuts at: 0 605 915
Size: 605 310
BsaHI Gr'CG_yC
Cuts at: 0 185 915
Size: 185 730
BsaWI w'CCGG_w
Cuts at: 0 541 915
Size: 541 374
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 190 915
Size: 190 725
BseYI C'CCAG_C
Cuts at: 0 839 915
Size: 839 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 915
Size: 67 848
BsiEI CG_ry'CG
Cuts at: 0 582 591 849 915
Size: 582 9 258 66
Fragments arranged by size:
582 258 66 9
BsiHKAI G_wGCw'C
Cuts at: 0 350 915
Size: 350 565
BsmBI CGTCTCn'nnnn_
Cuts at: 0 314 915
Size: 314 601
Bsp1286I G_dGCh'C
Cuts at: 0 279 350 800 915
Size: 279 71 450 115
Fragments arranged by size:
450 279 115 71
BsrBI CCG'CTC
Cuts at: 0 809 915
Size: 809 106
BtgI C'CryG_G
Cuts at: 0 191 212 368 464 915
Size: 191 21 156 96 451
Fragments arranged by size:
451 191 156 96 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 481 915
Size: 481 434
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 396 431 915
Size: 396 35 484
Fragments arranged by size:
484 396 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 57 915
Size: 57 858
EaeI y'GGCC_r
Cuts at: 0 72 194 579 588 915
Size: 72 122 385 9 327
Fragments arranged by size:
385 327 122 72 9
EagI C'GGCC_G
Cuts at: 0 579 588 915
Size: 579 9 327
Fragments arranged by size:
579 327 9
EarI CTCTTCn'nnn_
Cuts at: 0 799 915
Size: 799 116
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 196 692 915
Size: 196 496 223
Fragments arranged by size:
496 223 196
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 915
Size: 256 659
EcoNI CCTnn'n_nnAGG
Cuts at: 0 628 869 915
Size: 628 241 46
Fragments arranged by size:
628 241 46
EcoRI G'AATT_C
Cuts at: 0 6 915
Size: 6 909
EcoRV GAT'ATC
Cuts at: 0 534 915
Size: 534 381
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 315 915
Size: 315 600
HaeII r_GCGC'y
Cuts at: 0 138 915
Size: 138 777
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 385 417 523 555 915
Size: 385 32 106 32 360
Fragments arranged by size:
385 360 106 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 915
Size: 285 630
MspA1I CmG'CkG
Cuts at: 0 466 915
Size: 466 449
NcoI C'CATG_G
Cuts at: 0 191 915
Size: 191 724
PflMI CCAn_nnn'nTGG
Cuts at: 0 213 501 915
Size: 213 288 414
Fragments arranged by size:
414 288 213
PspOMI G'GGCC_C
Cuts at: 0 796 915
Size: 796 119
SacII CC_GC'GG
Cuts at: 0 467 915
Size: 467 448
SapI GCTCTTCn'nnn_
Cuts at: 0 799 915
Size: 799 116
SfcI C'TryA_G
Cuts at: 0 771 915
Size: 771 144
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 71 587 915
Size: 71 516 328
Fragments arranged by size:
516 328 71
SmaI CCC'GGG
Cuts at: 0 253 574 915
Size: 253 321 341
Fragments arranged by size:
341 321 253
StuI AGG'CCT
Cuts at: 0 85 311 915
Size: 85 226 604
Fragments arranged by size:
604 226 85
StyI C'CwwG_G
Cuts at: 0 191 284 524 629 915
Size: 191 93 240 105 286
Fragments arranged by size:
286 240 191 105 93
XmaI C'CCGG_G
Cuts at: 0 251 572 915
Size: 251 321 343
Fragments arranged by size:
343 321 251
Enzymes that do cut:
AcuI AfeI AhdI AloI AlwNI ApaI ApoI AvaI
BanI BanII BbvCI BcgI BglI Bme1580I BmgBI BplI
Bpu10I BsaI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BsrBI BtgI BtgZI CspCI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV
FalI HaeII Hin4I MmeI MspA1I NcoI PflMI PspOMI
SacII SapI SfcI SfiI SmaI StuI StyI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BciVI BclI BfrBI BglII BlpI
BmrI BmtI BpmI BpuEI BsaAI BsaBI BsaXI BsiWI
BsmI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtsI ClaI DraI DraIII EcoICRI EcoO109I FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI
SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmnI ZraI