Restriction map for
pF1KE0719
(Linear) (Six-base) MAPSORT of: KIEE0719.seq Check: 5683 from: 1 to: 915
KIEE0719.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 193 280 403 915
Size: 115 78 87 123 512
Fragments arranged by size:
512 123 115 87 78
AleI CACnn'nnGTG
Cuts at: 0 547 915
Size: 547 368
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 799 915
Size: 799 116
AlwNI CAG_nnn'CTG
Cuts at: 0 258 333 396 915
Size: 258 75 63 519
Fragments arranged by size:
519 258 75 63
ApaLI G'TGCA_C
Cuts at: 0 208 915
Size: 208 707
AvaI C'yCGr_G
Cuts at: 0 511 682 915
Size: 511 171 233
Fragments arranged by size:
511 233 171
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 484 517 915
Size: 484 33 398
Fragments arranged by size:
484 398 33
BanI G'GyrC_C
Cuts at: 0 37 415 459 915
Size: 37 378 44 456
Fragments arranged by size:
456 378 44 37
BanII G_rGCy'C
Cuts at: 0 296 872 915
Size: 296 576 43
Fragments arranged by size:
576 296 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 677 915
Size: 677 238
BbvCI CC'TCA_GC
Cuts at: 0 247 915
Size: 247 668
BglII A'GATC_T
Cuts at: 0 152 915
Size: 152 763
Bme1580I G_kGCm'C
Cuts at: 0 40 212 464 758 915
Size: 40 172 252 294 157
Fragments arranged by size:
294 252 172 157 40
BmgBI CAC'GTC
Cuts at: 0 204 915
Size: 204 711
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 784 915
Size: 579 205 131
Fragments arranged by size:
579 205 131
Bpu10I CC'TnA_GC
Cuts at: 0 247 866 915
Size: 247 619 49
Fragments arranged by size:
619 247 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 915
Size: 148 767
BsaI GGTCTCn'nnnn_
Cuts at: 0 371 915
Size: 371 544
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 259 289 552 582 915
Size: 259 30 263 30 333
Fragments arranged by size:
333 263 259 30 30
BseYI C'CCAG_C
Cuts at: 0 654 664 692 811 876 915
Size: 654 10 28 119 65 39
Fragments arranged by size:
654 119 65 39 28 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 915
Size: 286 629
BsiHKAI G_wGCw'C
Cuts at: 0 212 296 915
Size: 212 84 619
Fragments arranged by size:
619 212 84
Bsp1286I G_dGCh'C
Cuts at: 0 40 212 296 464 758 872 915
Size: 40 172 84 168 294 114 43
Fragments arranged by size:
294 172 168 114 84 43 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 404 791 915
Size: 404 387 124
Fragments arranged by size:
404 387 124
BsrGI T'GTAC_A
Cuts at: 0 53 915
Size: 53 862
BstAPI GCAn_nnn'nTGC
Cuts at: 0 569 798 915
Size: 569 229 117
Fragments arranged by size:
569 229 117
BstEII G'GTnAC_C
Cuts at: 0 687 915
Size: 687 228
BstXI CCAn_nnnn'nTGG
Cuts at: 0 426 549 915
Size: 426 123 366
Fragments arranged by size:
426 366 123
BstYI r'GATC_y
Cuts at: 0 152 915
Size: 152 763
BtsI GCAGTG_nn'
Cuts at: 0 137 915
Size: 137 778
EarI CTCTTCn'nnn_
Cuts at: 0 328 740 915
Size: 328 412 175
Fragments arranged by size:
412 328 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 898 915
Size: 898 17
EcoICRI GAG'CTC
Cuts at: 0 294 915
Size: 294 621
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 193 280 403 579 784 915
Size: 115 78 87 123 176 205 131
Fragments arranged by size:
205 176 131 123 115 87 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 250 915
Size: 250 665
EcoO109I rG'GnC_Cy
Cuts at: 0 129 255 780 915
Size: 129 126 525 135
Fragments arranged by size:
525 135 129 126
EcoRV GAT'ATC
Cuts at: 0 555 915
Size: 555 360
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 732 915
Size: 732 183
FspI TGC'GCA
Cuts at: 0 469 915
Size: 469 446
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 576 608 915
Size: 576 32 307
Fragments arranged by size:
576 307 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 915
Size: 216 699
MslI CAynn'nnrTG
Cuts at: 0 547 915
Size: 547 368
MspA1I CmG'CkG
Cuts at: 0 84 330 547 696 819 880 915
Size: 84 246 217 149 123 61 35
Fragments arranged by size:
246 217 149 123 84 61 35
PflMI CCAn_nnn'nTGG
Cuts at: 0 486 915
Size: 486 429
PpuMI rG'GwC_Cy
Cuts at: 0 129 255 780 915
Size: 129 126 525 135
Fragments arranged by size:
525 135 129 126
PstI C_TGCA'G
Cuts at: 0 338 803 902 915
Size: 338 465 99 13
Fragments arranged by size:
465 338 99 13
PvuII CAG'CTG
Cuts at: 0 84 330 547 696 880 915
Size: 84 246 217 149 184 35
Fragments arranged by size:
246 217 184 149 84 35
SacI G_AGCT'C
Cuts at: 0 296 915
Size: 296 619
SanDI GG'GwC_CC
Cuts at: 0 129 915
Size: 129 786
SfcI C'TryA_G
Cuts at: 0 334 799 898 915
Size: 334 465 99 17
Fragments arranged by size:
465 334 99 17
SmaI CCC'GGG
Cuts at: 0 684 915
Size: 684 231
SmlI C'TyrA_G
Cuts at: 0 163 915
Size: 163 752
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 403 493 678 706 915
Size: 403 90 185 28 209
Fragments arranged by size:
403 209 185 90 28
TatI w'GTAC_w
Cuts at: 0 53 266 915
Size: 53 213 649
Fragments arranged by size:
649 213 53
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 738 837 915
Size: 738 99 78
Fragments arranged by size:
738 99 78
XmaI C'CCGG_G
Cuts at: 0 682 915
Size: 682 233
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaLI AvaI BaeI BanI
BanII BbsI BbvCI BglII Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrGI BstAPI BstEII BstXI BstYI BtsI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI Hin4I
MmeI MslI MspA1I PflMI PpuMI PstI PvuII SacI
SanDI SfcI SmaI SmlI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaI ApoI AscI AseI AsiSI
AvrII BamHI BbeI BcgI BciVI BclI BfrBI BglI
BlpI BmrI BmtI BplI BsaAI BsaBI BsaHI BsaWI
BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstBI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EcoRI FseI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TstI Tth111I
XbaI XhoI XmnI ZraI