(Linear) (Six-base) MAPSORT of: KIEE0720.seq Check: 7311 from: 1 to: 1242 KIEE0720.orf With 182 enzymes: SgfI * April 24, 2009 13:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1058 1228 1242 Size: 1058 170 14 Fragments arranged by size: 1058 170 14 AccI GT'mk_AC Cuts at: 0 617 1242 Size: 617 625 Acc65I G'GTAC_C Cuts at: 0 416 1242 Size: 416 826 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 394 1242 Size: 394 848 AflIII A'CryG_T Cuts at: 0 284 474 713 1242 Size: 284 190 239 529 Fragments arranged by size: 529 284 239 190 AhdI GACnn_n'nnGTC Cuts at: 0 624 1242 Size: 624 618 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 271 303 856 888 1242 Size: 271 32 553 32 354 Fragments arranged by size: 553 354 271 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 75 1242 Size: 75 1167 ApoI r'AATT_y Cuts at: 0 1081 1242 Size: 1081 161 BamHI G'GATC_C Cuts at: 0 795 1242 Size: 795 447 BanI G'GyrC_C Cuts at: 0 416 544 1242 Size: 416 128 698 Fragments arranged by size: 698 416 128 BbvCI CC'TCA_GC Cuts at: 0 775 1242 Size: 775 467 BciVI GTATCCnnnnn_n' Cuts at: 0 595 1242 Size: 595 647 BfrBI ATG'CAT Cuts at: 0 113 1242 Size: 113 1129 Bme1580I G_kGCm'C Cuts at: 0 842 905 1242 Size: 842 63 337 Fragments arranged by size: 842 337 63 BmrI ACTGGGnnnn_n' Cuts at: 0 870 1242 Size: 870 372 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1123 1242 Size: 1123 119 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 253 421 548 684 1168 1242 Size: 253 168 127 136 484 74 Fragments arranged by size: 484 253 168 136 127 74 Bpu10I CC'TnA_GC Cuts at: 0 775 1108 1242 Size: 775 333 134 Fragments arranged by size: 775 333 134 BsaAI yAC'GTr Cuts at: 0 749 1242 Size: 749 493 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 271 301 727 757 1242 Size: 271 30 426 30 485 Fragments arranged by size: 485 426 271 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 292 732 1198 1242 Size: 292 440 466 44 Fragments arranged by size: 466 440 292 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 1206 1242 Size: 139 1067 36 Fragments arranged by size: 1067 139 36 BsiHKAI G_wGCw'C Cuts at: 0 239 1242 Size: 239 1003 BsiWI C'GTAC_G Cuts at: 0 671 1242 Size: 671 571 Bsp1286I G_dGCh'C Cuts at: 0 239 842 905 1242 Size: 239 603 63 337 Fragments arranged by size: 603 337 239 63 BspHI T'CATG_A Cuts at: 0 822 1242 Size: 822 420 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 311 329 1058 1228 1242 Size: 311 18 729 170 14 Fragments arranged by size: 729 311 170 18 14 BsrDI GCAATG_nn' Cuts at: 0 155 1242 Size: 155 1087 BsrGI T'GTAC_A Cuts at: 0 581 1242 Size: 581 661 BstAPI GCAn_nnn'nTGC Cuts at: 0 222 345 1242 Size: 222 123 897 Fragments arranged by size: 897 222 123 BstYI r'GATC_y Cuts at: 0 795 1006 1242 Size: 795 211 236 Fragments arranged by size: 795 236 211 BtgI C'CryG_G Cuts at: 0 536 1227 1242 Size: 536 691 15 Fragments arranged by size: 691 536 15 ClaI AT'CG_AT Cuts at: 0 698 1242 Size: 698 544 DraIII CAC_nnn'GTG Cuts at: 0 752 1242 Size: 752 490 EaeI y'GGCC_r Cuts at: 0 848 1242 Size: 848 394 EarI CTCTTCn'nnn_ Cuts at: 0 941 1242 Size: 941 301 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 253 394 421 548 684 1168 1242 Size: 253 141 27 127 136 484 74 Fragments arranged by size: 484 253 141 136 127 74 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 790 1242 Size: 790 452 EcoO109I rG'GnC_Cy Cuts at: 0 595 1242 Size: 595 647 EcoRI G'AATT_C Cuts at: 0 1081 1242 Size: 1081 161 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 610 642 780 812 1242 Size: 610 32 138 32 430 Fragments arranged by size: 610 430 138 32 32 HincII GTy'rAC Cuts at: 0 54 618 1242 Size: 54 564 624 Fragments arranged by size: 624 564 54 KpnI G_GTAC'C Cuts at: 0 420 1242 Size: 420 822 MslI CAynn'nnrTG Cuts at: 0 289 295 473 1242 Size: 289 6 178 769 Fragments arranged by size: 769 289 178 6 MspA1I CmG'CkG Cuts at: 0 1031 1038 1242 Size: 1031 7 204 Fragments arranged by size: 1031 204 7 NcoI C'CATG_G Cuts at: 0 536 1242 Size: 536 706 NsiI A_TGCA'T Cuts at: 0 115 1242 Size: 115 1127 NspI r_CATG'y Cuts at: 0 288 478 717 1242 Size: 288 190 239 525 Fragments arranged by size: 525 288 239 190 PasI CC'CwG_GG Cuts at: 0 630 1242 Size: 630 612 PciI A'CATG_T Cuts at: 0 284 474 713 1242 Size: 284 190 239 529 Fragments arranged by size: 529 284 239 190 PflMI CCAn_nnn'nTGG Cuts at: 0 1140 1242 Size: 1140 102 PfoI T'CCnGG_A Cuts at: 0 206 269 1073 1184 1242 Size: 206 63 804 111 58 Fragments arranged by size: 804 206 111 63 58 PmlI CAC'GTG Cuts at: 0 749 1242 Size: 749 493 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 271 303 1242 Size: 271 32 939 Fragments arranged by size: 939 271 32 PstI C_TGCA'G Cuts at: 0 218 338 1055 1237 1242 Size: 218 120 717 182 5 Fragments arranged by size: 717 218 182 120 5 PvuII CAG'CTG Cuts at: 0 1031 1242 Size: 1031 211 SalI G'TCGA_C Cuts at: 0 616 1242 Size: 616 626 SfcI C'TryA_G Cuts at: 0 214 334 1051 1233 1242 Size: 214 120 717 182 9 Fragments arranged by size: 717 214 182 120 9 StyI C'CwwG_G Cuts at: 0 536 791 1242 Size: 536 255 451 Fragments arranged by size: 536 451 255 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 675 1228 1242 Size: 675 553 14 Fragments arranged by size: 675 553 14 TatI w'GTAC_w Cuts at: 0 155 581 1242 Size: 155 426 661 Fragments arranged by size: 661 426 155 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 727 759 1242 Size: 727 32 483 Fragments arranged by size: 727 483 32 XbaI T'CTAG_A Cuts at: 0 957 1242 Size: 957 285 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 555 1242 Size: 555 687 XmnI GAAnn'nnTTC Cuts at: 0 202 1242 Size: 202 1040 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AhdI AloI AlwNI ApoI BamHI BanI BbvCI BciVI BfrBI Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaXI BseRI BsgI BsiHKAI BsiWI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstYI BtgI ClaI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII KpnI MslI MspA1I NcoI NsiI NspI PasI PciI PflMI PfoI PmlI PpiI PstI PvuII SalI SfcI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AgeI AleI AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanII BbeI BbsI BcgI BclI BglI BglII BlpI BmgBI BmtI BpuEI BsaI BsaBI BsaHI BsaWI BseYI BsiEI BsmI BsmBI BspEI BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI CspCI DraI DrdI EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PmeI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XhoI XmaI ZraI