Restriction map for
pF1KE0722
(Linear) (Six-base) MAPSORT of: KIEE0722.seq Check: 8610 from: 1 to: 1245
KIEE0722.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 178 492 1245
Size: 178 314 753
Fragments arranged by size:
753 314 178
AccI GT'mk_AC
Cuts at: 0 566 1028 1245
Size: 566 462 217
Fragments arranged by size:
566 462 217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 596 1245
Size: 596 649
AlwNI CAG_nnn'CTG
Cuts at: 0 976 1245
Size: 976 269
ApaI G_GGCC'C
Cuts at: 0 138 1245
Size: 138 1107
ApaLI G'TGCA_C
Cuts at: 0 694 1245
Size: 694 551
AvaI C'yCGr_G
Cuts at: 0 310 1009 1245
Size: 310 699 236
Fragments arranged by size:
699 310 236
BanI G'GyrC_C
Cuts at: 0 840 1245
Size: 840 405
BanII G_rGCy'C
Cuts at: 0 138 740 830 1245
Size: 138 602 90 415
Fragments arranged by size:
602 415 138 90
BbsI GAAGACnn'nnnn_
Cuts at: 0 427 1245
Size: 427 818
BglI GCCn_nnn'nGGC
Cuts at: 0 553 1245
Size: 553 692
Bme1580I G_kGCm'C
Cuts at: 0 138 698 823 845 1245
Size: 138 560 125 22 400
Fragments arranged by size:
560 400 138 125 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 225 1245
Size: 225 1020
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 1174 1245
Size: 610 564 71
Fragments arranged by size:
610 564 71
BsaI GGTCTCn'nnnn_
Cuts at: 0 985 1245
Size: 985 260
BsaHI Gr'CG_yC
Cuts at: 0 782 1245
Size: 782 463
BsaWI w'CCGG_w
Cuts at: 0 623 837 1245
Size: 623 214 408
Fragments arranged by size:
623 408 214
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 136 166 694 724 1245
Size: 136 30 528 30 521
Fragments arranged by size:
528 521 136 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 160 310 328 1245
Size: 130 30 150 18 917
Fragments arranged by size:
917 150 130 30 18
BseYI C'CCAG_C
Cuts at: 0 5 1245
Size: 5 1240
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 190 925 1066 1245
Size: 54 136 735 141 179
Fragments arranged by size:
735 179 141 136 54
BsiEI CG_ry'CG
Cuts at: 0 864 1245
Size: 864 381
BsiHKAI G_wGCw'C
Cuts at: 0 623 698 740 817 1245
Size: 623 75 42 77 428
Fragments arranged by size:
623 428 77 75 42
Bsp1286I G_dGCh'C
Cuts at: 0 138 623 698 740 817 823 830 845
Size: 138 485 75 42 77 6 7 15
Cuts at: 845 1245
Size: 400
Fragments arranged by size:
485 400 138 77 75 42 15 7
6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 178 492 1245
Size: 178 314 753
Fragments arranged by size:
753 314 178
BsrBI CCG'CTC
Cuts at: 0 150 561 835 1014 1245
Size: 150 411 274 179 231
Fragments arranged by size:
411 274 231 179 150
BsrDI GCAATG_nn'
Cuts at: 0 578 1245
Size: 578 667
BsrFI r'CCGG_y
Cuts at: 0 662 1245
Size: 662 583
BstAPI GCAn_nnn'nTGC
Cuts at: 0 976 1245
Size: 976 269
BstEII G'GTnAC_C
Cuts at: 0 870 1245
Size: 870 375
BstXI CCAn_nnnn'nTGG
Cuts at: 0 966 1245
Size: 966 279
BstZ17I GTA'TAC
Cuts at: 0 567 1245
Size: 567 678
Bsu36I CC'TnA_GG
Cuts at: 0 214 1245
Size: 214 1031
BtgI C'CryG_G
Cuts at: 0 407 1139 1245
Size: 407 732 106
Fragments arranged by size:
732 407 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1023 1245
Size: 1023 222
BtsI GCAGTG_nn'
Cuts at: 0 538 724 1049 1245
Size: 538 186 325 196
Fragments arranged by size:
538 325 196 186
EaeI y'GGCC_r
Cuts at: 0 192 489 545 1245
Size: 192 297 56 700
Fragments arranged by size:
700 297 192 56
EarI CTCTTCn'nnn_
Cuts at: 0 373 505 1245
Size: 373 132 740
Fragments arranged by size:
740 373 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 157 1245
Size: 157 1088
EcoICRI GAG'CTC
Cuts at: 0 738 1245
Size: 738 507
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 1174 1245
Size: 610 564 71
Fragments arranged by size:
610 564 71
EcoNI CCTnn'n_nnAGG
Cuts at: 0 292 1245
Size: 292 953
EcoO109I rG'GnC_Cy
Cuts at: 0 134 218 1245
Size: 134 84 1027
Fragments arranged by size:
1027 134 84
FspI TGC'GCA
Cuts at: 0 584 1245
Size: 584 661
HaeII r_GCGC'y
Cuts at: 0 485 1096 1245
Size: 485 611 149
Fragments arranged by size:
611 485 149
HindIII A'AGCT_T
Cuts at: 0 1240 1245
Size: 1240 5
MscI TGG'CCA
Cuts at: 0 194 1245
Size: 194 1051
MslI CAynn'nnrTG
Cuts at: 0 1042 1245
Size: 1042 203
MspA1I CmG'CkG
Cuts at: 0 9 78 276 588 648 879 1141 1245
Size: 9 69 198 312 60 231 262 104
Fragments arranged by size:
312 262 231 198 104 69 60 9
NcoI C'CATG_G
Cuts at: 0 407 1245
Size: 407 838
PfoI T'CCnGG_A
Cuts at: 0 1151 1163 1245
Size: 1151 12 82
Fragments arranged by size:
1151 82 12
PspOMI G'GGCC_C
Cuts at: 0 134 1245
Size: 134 1111
PstI C_TGCA'G
Cuts at: 0 14 77 775 1245
Size: 14 63 698 470
Fragments arranged by size:
698 470 63 14
PvuI CG_AT'CG
Cuts at: 0 864 1245
Size: 864 381
PvuII CAG'CTG
Cuts at: 0 9 276 588 648 1245
Size: 9 267 312 60 597
Fragments arranged by size:
597 312 267 60 9
SacI G_AGCT'C
Cuts at: 0 740 1245
Size: 740 505
SacII CC_GC'GG
Cuts at: 0 1142 1245
Size: 1142 103
SapI GCTCTTCn'nnn_
Cuts at: 0 373 1245
Size: 373 872
SfcI C'TryA_G
Cuts at: 0 10 73 771 1245
Size: 10 63 698 474
Fragments arranged by size:
698 474 63 10
SmlI C'TyrA_G
Cuts at: 0 310 1245
Size: 310 935
StuI AGG'CCT
Cuts at: 0 466 640 1245
Size: 466 174 605
Fragments arranged by size:
605 466 174
StyI C'CwwG_G
Cuts at: 0 407 1245
Size: 407 838
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 681 1245
Size: 681 564
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 174 206 618 650 1008 1040 1168 1200
Size: 174 32 412 32 358 32 128 32
Cuts at: 1200 1245
Size: 45
Fragments arranged by size:
412 358 174 128 45 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 370 1245
Size: 370 875
XhoI C'TCGA_G
Cuts at: 0 310 1245
Size: 310 935
Enzymes that do cut:
AarI AccI AlfI AlwNI ApaI ApaLI AvaI BanI
BanII BbsI BglI Bme1580I BplI BpmI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI BtsI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FspI HaeII HindIII MscI MslI
MspA1I NcoI PfoI PspOMI PstI PvuI PvuII SacI
SacII SapI SfcI SmlI StuI StyI TaqII TstI
Tth111I XhoI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaAI
BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII
BssSI BstBI BstYI ClaI CspCI DraI DraIII DrdI
EagI EcoRI EcoRV FalI FseI FspAI Hin4I HincII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI RsrII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI XbaI XcmI XmaI
XmnI ZraI