Restriction map for
pF1KE0723
(Linear) (Six-base) MAPSORT of: KIEE0723.seq Check: 3791 from: 1 to: 918
KIEE0723.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 48 445 918
Size: 48 397 473
Fragments arranged by size:
473 397 48
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 574 650 918
Size: 574 76 268
Fragments arranged by size:
574 268 76
AlwNI CAG_nnn'CTG
Cuts at: 0 252 316 918
Size: 252 64 602
Fragments arranged by size:
602 252 64
ApaLI G'TGCA_C
Cuts at: 0 157 918
Size: 157 761
AvaI C'yCGr_G
Cuts at: 0 62 203 443 824 918
Size: 62 141 240 381 94
Fragments arranged by size:
381 240 141 94 62
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 467 500 918
Size: 467 33 418
Fragments arranged by size:
467 418 33
BanI G'GyrC_C
Cuts at: 0 685 918
Size: 685 233
BanII G_rGCy'C
Cuts at: 0 851 918
Size: 851 67
BbeI G_GCGC'C
Cuts at: 0 689 918
Size: 689 229
BbsI GAAGACnn'nnnn_
Cuts at: 0 60 918
Size: 60 858
BlpI GC'TnA_GC
Cuts at: 0 528 613 918
Size: 528 85 305
Fragments arranged by size:
528 305 85
Bme1580I G_kGCm'C
Cuts at: 0 161 419 918
Size: 161 258 499
Fragments arranged by size:
499 258 161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 918
Size: 290 628
Bpu10I CC'TnA_GC
Cuts at: 0 278 918
Size: 278 640
BsaI GGTCTCn'nnnn_
Cuts at: 0 109 918
Size: 109 809
BsaHI Gr'CG_yC
Cuts at: 0 686 918
Size: 686 232
BseYI C'CCAG_C
Cuts at: 0 216 918
Size: 216 702
BsiHKAI G_wGCw'C
Cuts at: 0 161 918
Size: 161 757
BsmBI CGTCTCn'nnnn_
Cuts at: 0 821 918
Size: 821 97
Bsp1286I G_dGCh'C
Cuts at: 0 161 419 851 918
Size: 161 258 432 67
Fragments arranged by size:
432 258 161 67
BsrBI CCG'CTC
Cuts at: 0 506 918
Size: 506 412
BsrDI GCAATG_nn'
Cuts at: 0 30 918
Size: 30 888
BsrFI r'CCGG_y
Cuts at: 0 145 688 758 918
Size: 145 543 70 160
Fragments arranged by size:
543 160 145 70
BstAPI GCAn_nnn'nTGC
Cuts at: 0 316 918
Size: 316 602
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 851 918
Size: 851 67
BtsI GCAGTG_nn'
Cuts at: 0 558 735 918
Size: 558 177 183
Fragments arranged by size:
558 183 177
ClaI AT'CG_AT
Cuts at: 0 602 918
Size: 602 316
EaeI y'GGCC_r
Cuts at: 0 135 143 239 760 918
Size: 135 8 96 521 158
Fragments arranged by size:
521 158 135 96 8
EarI CTCTTCn'nnn_
Cuts at: 0 385 863 918
Size: 385 478 55
Fragments arranged by size:
478 385 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 918
Size: 290 628
EcoNI CCTnn'n_nnAGG
Cuts at: 0 805 918
Size: 805 113
EcoO109I rG'GnC_Cy
Cuts at: 0 908 918
Size: 908 10
HaeII r_GCGC'y
Cuts at: 0 198 689 918
Size: 198 491 229
Fragments arranged by size:
491 229 198
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 431 463 918
Size: 34 32 365 32 455
Fragments arranged by size:
455 365 34 32 32
HindIII A'AGCT_T
Cuts at: 0 10 918
Size: 10 908
KasI G'GCGC_C
Cuts at: 0 685 918
Size: 685 233
MslI CAynn'nnrTG
Cuts at: 0 262 918
Size: 262 656
MspA1I CmG'CkG
Cuts at: 0 211 514 716 918
Size: 211 303 202 202
Fragments arranged by size:
303 211 202 202
NaeI GCC'GGC
Cuts at: 0 147 690 760 918
Size: 147 543 70 158
Fragments arranged by size:
543 158 147 70
NarI GG'CG_CC
Cuts at: 0 686 918
Size: 686 232
NgoMIV G'CCGG_C
Cuts at: 0 145 688 758 918
Size: 145 543 70 160
Fragments arranged by size:
543 160 145 70
PasI CC'CwG_GG
Cuts at: 0 419 807 918
Size: 419 388 111
Fragments arranged by size:
419 388 111
PstI C_TGCA'G
Cuts at: 0 563 918
Size: 563 355
PvuII CAG'CTG
Cuts at: 0 211 514 918
Size: 211 303 404
Fragments arranged by size:
404 303 211
SapI GCTCTTCn'nnn_
Cuts at: 0 385 863 918
Size: 385 478 55
Fragments arranged by size:
478 385 55
ScaI AGT'ACT
Cuts at: 0 184 918
Size: 184 734
SfcI C'TryA_G
Cuts at: 0 559 918
Size: 559 359
SfoI GGC'GCC
Cuts at: 0 687 918
Size: 687 231
SgrAI Cr'CCGG_yG
Cuts at: 0 688 918
Size: 688 230
SmaI CCC'GGG
Cuts at: 0 205 445 918
Size: 205 240 473
Fragments arranged by size:
473 240 205
SmlI C'TyrA_G
Cuts at: 0 62 918
Size: 62 856
SrfI GCCC'GGGC
Cuts at: 0 205 918
Size: 205 713
StuI AGG'CCT
Cuts at: 0 800 918
Size: 800 118
StyI C'CwwG_G
Cuts at: 0 293 918
Size: 293 625
TatI w'GTAC_w
Cuts at: 0 182 918
Size: 182 736
XhoI C'TCGA_G
Cuts at: 0 62 918
Size: 62 856
XmaI C'CCGG_G
Cuts at: 0 203 443 918
Size: 203 240 475
Fragments arranged by size:
475 240 203
Enzymes that do cut:
AhdI AlfI AlwNI ApaLI AvaI BaeI BanI BanII
BbeI BbsI BlpI Bme1580I BpmI Bpu10I BsaI BsaHI
BseYI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrFI BstAPI
BtgZI BtsI ClaI EaeI EarI Eco57MI EcoNI EcoO109I
HaeII Hin4I HindIII KasI MslI MspA1I NaeI NarI
NgoMIV PasI PstI PvuII SapI ScaI SfcI SfoI
SgrAI SmaI SmlI SrfI StuI StyI TatI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AloI ApaI ApoI AscI AseI
AsiSI AvrII BamHI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmrI BmtI BplI BpuEI BsaAI
BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiWI BsmI
BspEI BspHI BspMI BsrGI BssHII BssSI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HincII HpaI KpnI MfeI MluI MmeI
MscI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI SexAI SfiI SgfI SnaBI
SpeI SphI SspI SwaI TaqII TstI Tth111I XbaI
XcmI XmnI ZraI