Restriction map for
pF1KE0728
(Linear) (Six-base) MAPSORT of: KIEE0728.seq Check: 4234 from: 1 to: 1254
KIEE0728.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 881 1254
Size: 881 373
AccI GT'mk_AC
Cuts at: 0 713 1254
Size: 713 541
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 891 1254
Size: 420 471 363
Fragments arranged by size:
471 420 363
AfeI AGC'GCT
Cuts at: 0 303 1254
Size: 303 951
AflIII A'CryG_T
Cuts at: 0 639 1254
Size: 639 615
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1000 1254
Size: 1000 254
AlwNI CAG_nnn'CTG
Cuts at: 0 106 1254
Size: 106 1148
ApoI r'AATT_y
Cuts at: 0 724 1254
Size: 724 530
AvaI C'yCGr_G
Cuts at: 0 235 1254
Size: 235 1019
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1224 1254
Size: 1224 30
BamHI G'GATC_C
Cuts at: 0 447 1254
Size: 447 807
BanI G'GyrC_C
Cuts at: 0 319 592 918 1126 1254
Size: 319 273 326 208 128
Fragments arranged by size:
326 319 273 208 128
BanII G_rGCy'C
Cuts at: 0 289 1254
Size: 289 965
BbeI G_GCGC'C
Cuts at: 0 922 1254
Size: 922 332
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 538 572 712 746 1254
Size: 538 34 140 34 508
Fragments arranged by size:
538 508 140 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 178 842 1225 1254
Size: 178 664 383 29
Fragments arranged by size:
664 383 178 29
BglI GCCn_nnn'nGGC
Cuts at: 0 411 1254
Size: 411 843
BlpI GC'TnA_GC
Cuts at: 0 125 1254
Size: 125 1129
Bme1580I G_kGCm'C
Cuts at: 0 789 1254
Size: 789 465
BmrI ACTGGGnnnn_n'
Cuts at: 0 138 785 1254
Size: 138 647 469
Fragments arranged by size:
647 469 138
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 537 1254
Size: 537 717
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1088 1254
Size: 1088 166
BsaI GGTCTCn'nnnn_
Cuts at: 0 1195 1254
Size: 1195 59
BsaAI yAC'GTr
Cuts at: 0 640 1254
Size: 640 614
BsaHI Gr'CG_yC
Cuts at: 0 7 919 1254
Size: 7 912 335
Fragments arranged by size:
912 335 7
BsaWI w'CCGG_w
Cuts at: 0 450 1254
Size: 450 804
BseYI C'CCAG_C
Cuts at: 0 1019 1254
Size: 1019 235
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 728 1254
Size: 728 526
BsiEI CG_ry'CG
Cuts at: 0 854 888 1254
Size: 854 34 366
Fragments arranged by size:
854 366 34
BsiHKAI G_wGCw'C
Cuts at: 0 242 1254
Size: 242 1012
BsmI GAATG_Cn'
Cuts at: 0 1140 1254
Size: 1140 114
BsmBI CGTCTCn'nnnn_
Cuts at: 0 14 318 1254
Size: 14 304 936
Fragments arranged by size:
936 304 14
Bsp1286I G_dGCh'C
Cuts at: 0 242 289 789 1254
Size: 242 47 500 465
Fragments arranged by size:
500 465 242 47
BspEI T'CCGG_A
Cuts at: 0 450 1254
Size: 450 804
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 881 1254
Size: 881 373
BsrDI GCAATG_nn'
Cuts at: 0 821 1204 1254
Size: 821 383 50
Fragments arranged by size:
821 383 50
BsrFI r'CCGG_y
Cuts at: 0 54 1254
Size: 54 1200
BsrGI T'GTAC_A
Cuts at: 0 779 896 1254
Size: 779 117 358
Fragments arranged by size:
779 358 117
BssSI C'ACGA_G
Cuts at: 0 297 1254
Size: 297 957
BstAPI GCAn_nnn'nTGC
Cuts at: 0 106 1254
Size: 106 1148
BstBI TT'CG_AA
Cuts at: 0 548 1254
Size: 548 706
BstYI r'GATC_y
Cuts at: 0 447 769 1254
Size: 447 322 485
Fragments arranged by size:
485 447 322
BtgI C'CryG_G
Cuts at: 0 173 227 596 848 882 914 1254
Size: 173 54 369 252 34 32 340
Fragments arranged by size:
369 340 252 173 54 34 32
BtsI GCAGTG_nn'
Cuts at: 0 1034 1254
Size: 1034 220
EaeI y'GGCC_r
Cuts at: 0 357 851 1254
Size: 357 494 403
Fragments arranged by size:
494 403 357
EagI C'GGCC_G
Cuts at: 0 851 1254
Size: 851 403
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 307 962 1254
Size: 307 655 292
Fragments arranged by size:
655 307 292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 891 1088 1254
Size: 420 471 197 166
Fragments arranged by size:
471 420 197 166
EcoRI G'AATT_C
Cuts at: 0 724 1254
Size: 724 530
HaeII r_GCGC'y
Cuts at: 0 305 922 1254
Size: 305 617 332
Fragments arranged by size:
617 332 305
HincII GTy'rAC
Cuts at: 0 666 1254
Size: 666 588
KasI G'GCGC_C
Cuts at: 0 918 1254
Size: 918 336
MscI TGG'CCA
Cuts at: 0 359 1254
Size: 359 895
MslI CAynn'nnrTG
Cuts at: 0 32 1178 1254
Size: 32 1146 76
Fragments arranged by size:
1146 76 32
MspA1I CmG'CkG
Cuts at: 0 106 175 850 884 916 1042 1254
Size: 106 69 675 34 32 126 212
Fragments arranged by size:
675 212 126 106 69 34 32
NarI GG'CG_CC
Cuts at: 0 919 1254
Size: 919 335
NotI GC'GGCC_GC
Cuts at: 0 851 1254
Size: 851 403
NspI r_CATG'y
Cuts at: 0 421 1254
Size: 421 833
PfoI T'CCnGG_A
Cuts at: 0 72 443 1254
Size: 72 371 811
Fragments arranged by size:
811 371 72
PmlI CAC'GTG
Cuts at: 0 640 1254
Size: 640 614
PstI C_TGCA'G
Cuts at: 0 992 1254
Size: 992 262
SacII CC_GC'GG
Cuts at: 0 176 851 885 917 1254
Size: 176 675 34 32 337
Fragments arranged by size:
675 337 176 34 32
SfcI C'TryA_G
Cuts at: 0 714 988 1047 1254
Size: 714 274 59 207
Fragments arranged by size:
714 274 207 59
SfoI GGC'GCC
Cuts at: 0 920 1254
Size: 920 334
SmlI C'TyrA_G
Cuts at: 0 11 1254
Size: 11 1243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 694 1254
Size: 694 560
TatI w'GTAC_w
Cuts at: 0 212 779 896 1254
Size: 212 567 117 358
Fragments arranged by size:
567 358 212 117
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlfI AlwNI ApoI
AvaI BaeI BamHI BanI BanII BbeI BcgI BciVI
BglI BlpI Bme1580I BmrI BplI BpmI BsaI BsaAI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtsI EaeI EagI EciI Eco57MI
EcoRI HaeII HincII KasI MscI MslI MspA1I NarI
NotI NspI PfoI PmlI PstI SacII SfcI SfoI
SmlI TaqII TatI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BbsI BbvCI
BclI BfrBI BglII BmgBI BmtI Bpu10I BpuEI BsaBI
BsaXI BseRI BsiWI BspHI BsrBI BssHII BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EarI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI
FspAI Hin4I HindIII HpaI KpnI MfeI MluI MmeI
NaeI NcoI NdeI NgoMIV NheI NruI NsiI PacI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI