Restriction map for
pF1KE0745
(Linear) (Six-base) MAPSORT of: KIEE0745.seq Check: 6469 from: 1 to: 960
KIEE0745.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 0 565 960
Size: 565 395
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 960
Size: 237 723
AflIII A'CryG_T
Cuts at: 0 748 960
Size: 748 212
ApaI G_GGCC'C
Cuts at: 0 530 960
Size: 530 430
BamHI G'GATC_C
Cuts at: 0 98 960
Size: 98 862
BanII G_rGCy'C
Cuts at: 0 89 153 530 773 960
Size: 89 64 377 243 187
Fragments arranged by size:
377 243 187 89 64
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 859 893 960
Size: 859 34 67
Fragments arranged by size:
859 67 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 510 960
Size: 510 450
Bme1580I G_kGCm'C
Cuts at: 0 530 960
Size: 530 430
BmrI ACTGGGnnnn_n'
Cuts at: 0 23 960
Size: 23 937
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 960
Size: 29 931
Bpu10I CC'TnA_GC
Cuts at: 0 812 960
Size: 812 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 430 960
Size: 76 354 530
Fragments arranged by size:
530 354 76
BsaI GGTCTCn'nnnn_
Cuts at: 0 256 748 960
Size: 256 492 212
Fragments arranged by size:
492 256 212
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 67 79 436 439 960
Size: 64 3 12 357 3 521
Fragments arranged by size:
521 357 64 12 3 3
BseYI C'CCAG_C
Cuts at: 0 522 555 960
Size: 522 33 405
Fragments arranged by size:
522 405 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 960
Size: 628 332
BsiHKAI G_wGCw'C
Cuts at: 0 89 153 773 960
Size: 89 64 620 187
Fragments arranged by size:
620 187 89 64
Bsp1286I G_dGCh'C
Cuts at: 0 89 153 530 773 960
Size: 89 64 377 243 187
Fragments arranged by size:
377 243 187 89 64
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 770 960
Size: 770 190
BsrDI GCAATG_nn'
Cuts at: 0 887 960
Size: 887 73
BsrFI r'CCGG_y
Cuts at: 0 603 960
Size: 603 357
BstBI TT'CG_AA
Cuts at: 0 22 960
Size: 22 938
BstYI r'GATC_y
Cuts at: 0 98 960
Size: 98 862
BtgI C'CryG_G
Cuts at: 0 672 960
Size: 672 288
BtsI GCAGTG_nn'
Cuts at: 0 154 960
Size: 154 806
DrdI GACnn_nn'nnGTC
Cuts at: 0 73 960
Size: 73 887
EaeI y'GGCC_r
Cuts at: 0 107 350 545 828 960
Size: 107 243 195 283 132
Fragments arranged by size:
283 243 195 132 107
EarI CTCTTCn'nnn_
Cuts at: 0 778 960
Size: 778 182
EcoICRI GAG'CTC
Cuts at: 0 87 151 771 960
Size: 87 64 620 189
Fragments arranged by size:
620 189 87 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 237 960
Size: 29 208 723
Fragments arranged by size:
723 208 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 811 960
Size: 811 149
EcoO109I rG'GnC_Cy
Cuts at: 0 405 429 526 960
Size: 405 24 97 434
Fragments arranged by size:
434 405 97 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 361 393 960
Size: 361 32 567
Fragments arranged by size:
567 361 32
MluI A'CGCG_T
Cuts at: 0 748 960
Size: 748 212
MscI TGG'CCA
Cuts at: 0 109 352 830 960
Size: 109 243 478 130
Fragments arranged by size:
478 243 130 109
MspA1I CmG'CkG
Cuts at: 0 503 555 674 960
Size: 503 52 119 286
Fragments arranged by size:
503 286 119 52
PpuMI rG'GwC_Cy
Cuts at: 0 405 429 960
Size: 405 24 531
Fragments arranged by size:
531 405 24
PspOMI G'GGCC_C
Cuts at: 0 526 960
Size: 526 434
PstI C_TGCA'G
Cuts at: 0 767 960
Size: 767 193
PvuII CAG'CTG
Cuts at: 0 555 960
Size: 555 405
SacI G_AGCT'C
Cuts at: 0 89 153 773 960
Size: 89 64 620 187
Fragments arranged by size:
620 187 89 64
SacII CC_GC'GG
Cuts at: 0 675 960
Size: 675 285
SapI GCTCTTCn'nnn_
Cuts at: 0 778 960
Size: 778 182
SbfI CC_TGCA'GG
Cuts at: 0 767 960
Size: 767 193
SexAI A'CCwGG_T
Cuts at: 0 41 960
Size: 41 919
SfcI C'TryA_G
Cuts at: 0 763 960
Size: 763 197
SmlI C'TyrA_G
Cuts at: 0 91 445 960
Size: 91 354 515
Fragments arranged by size:
515 354 91
StyI C'CwwG_G
Cuts at: 0 167 836 960
Size: 167 669 124
Fragments arranged by size:
669 167 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 772 960
Size: 772 188
TatI w'GTAC_w
Cuts at: 0 206 960
Size: 206 754
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 332 364 960
Size: 332 32 596
Fragments arranged by size:
596 332 32
Tth111I GACn'n_nGTC
Cuts at: 0 373 960
Size: 373 587
XmnI GAAnn'nnTTC
Cuts at: 0 310 960
Size: 310 650
Enzymes that do cut:
AccI AcuI AflIII ApaI BamHI BanII BcgI BciVI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BstBI BstYI
BtgI BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I Hin4I MluI MscI MspA1I PpuMI PspOMI PstI
PvuII SacI SacII SapI SbfI SexAI SfcI SmlI
StyI TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbsI BbvCI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII EagI EciI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MmeI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SanDI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI XbaI XcmI XhoI XmaI ZraI