Restriction map for
pF1KE0751
(Linear) (Six-base) MAPSORT of: KIEE0751.seq Check: 1988 from: 1 to: 984
KIEE0751.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AflIII A'CryG_T
Cuts at: 0 623 984
Size: 623 361
AleI CACnn'nnGTG
Cuts at: 0 364 984
Size: 364 620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 661 984
Size: 661 323
AlwNI CAG_nnn'CTG
Cuts at: 0 841 984
Size: 841 143
ApaI G_GGCC'C
Cuts at: 0 600 984
Size: 600 384
ApoI r'AATT_y
Cuts at: 0 127 984
Size: 127 857
AvaI C'yCGr_G
Cuts at: 0 27 599 819 921 984
Size: 27 572 220 102 63
Fragments arranged by size:
572 220 102 63 27
BanI G'GyrC_C
Cuts at: 0 22 984
Size: 22 962
BanII G_rGCy'C
Cuts at: 0 600 922 984
Size: 600 322 62
Fragments arranged by size:
600 322 62
BciVI GTATCCnnnnn_n'
Cuts at: 0 970 984
Size: 970 14
BlpI GC'TnA_GC
Cuts at: 0 554 984
Size: 554 430
Bme1580I G_kGCm'C
Cuts at: 0 600 984
Size: 600 384
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 984
Size: 165 819
BsaWI w'CCGG_w
Cuts at: 0 877 984
Size: 877 107
BseYI C'CCAG_C
Cuts at: 0 47 213 648 732 984
Size: 47 166 435 84 252
Fragments arranged by size:
435 252 166 84 47
Bsp1286I G_dGCh'C
Cuts at: 0 600 922 984
Size: 600 322 62
Fragments arranged by size:
600 322 62
BspEI T'CCGG_A
Cuts at: 0 877 984
Size: 877 107
BspHI T'CATG_A
Cuts at: 0 155 984
Size: 155 829
BsrDI GCAATG_nn'
Cuts at: 0 422 984
Size: 422 562
BstXI CCAn_nnnn'nTGG
Cuts at: 0 666 984
Size: 666 318
BstYI r'GATC_y
Cuts at: 0 162 984
Size: 162 822
BtgI C'CryG_G
Cuts at: 0 617 681 933 984
Size: 617 64 252 51
Fragments arranged by size:
617 252 64 51
EaeI y'GGCC_r
Cuts at: 0 678 902 930 984
Size: 678 224 28 54
Fragments arranged by size:
678 224 54 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 984
Size: 165 819
EcoO109I rG'GnC_Cy
Cuts at: 0 838 984
Size: 838 146
EcoRV GAT'ATC
Cuts at: 0 655 984
Size: 655 329
HaeII r_GCGC'y
Cuts at: 0 38 984
Size: 38 946
MluI A'CGCG_T
Cuts at: 0 623 984
Size: 623 361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 818 984
Size: 818 166
MslI CAynn'nnrTG
Cuts at: 0 364 515 643 984
Size: 364 151 128 341
Fragments arranged by size:
364 341 151 128
MspA1I CmG'CkG
Cuts at: 0 739 763 984
Size: 739 24 221
Fragments arranged by size:
739 221 24
NcoI C'CATG_G
Cuts at: 0 617 984
Size: 617 367
PflMI CCAn_nnn'nTGG
Cuts at: 0 709 984
Size: 709 275
PpuMI rG'GwC_Cy
Cuts at: 0 838 984
Size: 838 146
PspOMI G'GGCC_C
Cuts at: 0 596 984
Size: 596 388
SmaI CCC'GGG
Cuts at: 0 601 821 984
Size: 601 220 163
Fragments arranged by size:
601 220 163
SspI AAT'ATT
Cuts at: 0 335 385 984
Size: 335 50 599
Fragments arranged by size:
599 335 50
StyI C'CwwG_G
Cuts at: 0 18 617 984
Size: 18 599 367
Fragments arranged by size:
599 367 18
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 940 984
Size: 940 44
XmaI C'CCGG_G
Cuts at: 0 599 819 984
Size: 599 220 165
Fragments arranged by size:
599 220 165
Enzymes that do cut:
AflIII AleI AlfI AlwNI ApaI ApoI AvaI BanI
BanII BciVI BlpI Bme1580I BpmI BsaWI BseYI Bsp1286I
BspEI BspHI BsrDI BstXI BstYI BtgI EaeI Eco57MI
EcoO109I EcoRV HaeII MluI MmeI MslI MspA1I NcoI
PflMI PpuMI PspOMI SmaI SspI StyI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BclI BfrBI
BglI BglII BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaXI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI StuI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmnI ZraI