(Linear) (Six-base) MAPSORT of: KIEE0753.seq  Check: 3670  from: 1  to: 1014



KIEE0753.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AccI GT'mk_AC

Cuts at:      0     293    1014 
   Size:        293     721
 
AfeI AGC'GCT

Cuts at:      0     346    1014 
   Size:        346     668
 
AleI CACnn'nnGTG

Cuts at:      0     724    1014 
   Size:        724     290
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     586     618    1014 
   Size:        586      32     396

  Fragments arranged by size: 

                 586     396      32
 
ApaLI G'TGCA_C

Cuts at:      0     433    1014 
   Size:        433     581
 
AvaI C'yCGr_G

Cuts at:      0     287    1014 
   Size:        287     727
 
BamHI G'GATC_C

Cuts at:      0     338    1014 
   Size:        338     676
 
BanI G'GyrC_C

Cuts at:      0     546    1014 
   Size:        546     468
 
BanII G_rGCy'C

Cuts at:      0     368    1014 
   Size:        368     646
 
BbvCI CC'TCA_GC

Cuts at:      0     610    1014 
   Size:        610     404
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     411     445    1014 
   Size:        411      34     569

  Fragments arranged by size: 

                 569     411      34
 
BglII A'GATC_T

Cuts at:      0     653    1014 
   Size:        653     361
 
Bme1580I G_kGCm'C

Cuts at:      0     437    1014 
   Size:        437     577
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     302    1014 
   Size:        302     712
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     717     829    1014 
   Size:        717     112     185

  Fragments arranged by size: 

                 717     185     112
 
Bpu10I CC'TnA_GC

Cuts at:      0     314     610     923    1014 
   Size:        314     296     313      91

  Fragments arranged by size: 

                 314     313     296      91
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     895     973    1014 
   Size:        895      78      41

  Fragments arranged by size: 

                 895      78      41
 
BsaBI GATnn'nnATC

Cuts at:      0     191    1014 
   Size:        191     823
 
BsaHI Gr'CG_yC

Cuts at:      0     707    1014 
   Size:        707     307
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     475     505    1014 
   Size:        475      30     509

  Fragments arranged by size: 

                 509     475      30
 
BseYI C'CCAG_C

Cuts at:      0     389     448     801    1014 
   Size:        389      59     353     213

  Fragments arranged by size: 

                 389     353     213      59
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400    1014 
   Size:        400     614
 
BsiHKAI G_wGCw'C

Cuts at:      0     283     437     911    1014 
   Size:        283     154     474     103

  Fragments arranged by size: 

                 474     283     154     103
 
Bsp1286I G_dGCh'C

Cuts at:      0     283     368     437     911    1014 
   Size:        283      85      69     474     103

  Fragments arranged by size: 

                 474     283     103      85      69
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     422    1014 
   Size:        422     592
 
BsrDI GCAATG_nn'

Cuts at:      0     404    1014 
   Size:        404     610
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     882    1014 
   Size:        882     132
 
BstYI r'GATC_y

Cuts at:      0     338     653    1014 
   Size:        338     315     361

  Fragments arranged by size: 

                 361     338     315
 
BstZ17I GTA'TAC

Cuts at:      0     294    1014 
   Size:        294     720
 
BtgI C'CryG_G

Cuts at:      0      96    1014 
   Size:         96     918
 
EaeI y'GGCC_r

Cuts at:      0      93    1014 
   Size:         93     921
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     717     829    1014 
   Size:        717     112     185

  Fragments arranged by size: 

                 717     185     112
 
EcoO109I rG'GnC_Cy

Cuts at:      0     353    1014 
   Size:        353     661
 
EcoRV GAT'ATC

Cuts at:      0     521    1014 
   Size:        521     493
 
HaeII r_GCGC'y

Cuts at:      0     348    1014 
   Size:        348     666
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     268     281     300     313    1014 
   Size:        268      13      19      13     701

  Fragments arranged by size: 

                 701     268      19      13      13
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     461    1014 
   Size:        461     553
 
MscI TGG'CCA

Cuts at:      0      95    1014 
   Size:         95     919
 
MslI CAynn'nnrTG

Cuts at:      0     724    1014 
   Size:        724     290
 
MspA1I CmG'CkG

Cuts at:      0     393    1014 
   Size:        393     621
 
NcoI C'CATG_G

Cuts at:      0      96    1014 
   Size:         96     918
 
PasI CC'CwG_GG

Cuts at:      0     399    1014 
   Size:        399     615
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     564    1014 
   Size:        564     450
 
PvuII CAG'CTG

Cuts at:      0     393    1014 
   Size:        393     621
 
SmlI C'TyrA_G

Cuts at:      0     287     910     952    1014 
   Size:        287     623      42      62

  Fragments arranged by size: 

                 623     287      62      42
 
StuI AGG'CCT

Cuts at:      0     181    1014 
   Size:        181     833
 
StyI C'CwwG_G

Cuts at:      0      96    1014 
   Size:         96     918
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     894     949    1014 
   Size:        894      55      65

  Fragments arranged by size: 

                 894      65      55
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     103     622    1014 
   Size:        103     519     392

  Fragments arranged by size: 

                 519     392     103
 
XhoI C'TCGA_G

Cuts at:      0     287    1014 
   Size:        287     727
 

 Enzymes that do cut:

     AccI     AfeI     AleI     AloI    ApaLI     AvaI    BamHI     BanI
    BanII    BbvCI     BcgI    BglII Bme1580I     BplI     BpmI   Bpu10I
    BpuEI    BsaBI    BsaHI    BsaXI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrDI    BstXI    BstYI  BstZ17I     BtgI     EaeI  Eco57MI
 EcoO109I    EcoRV    HaeII    Hin4I     MmeI     MscI     MslI   MspA1I
     NcoI     PasI    PflMI    PvuII     SmlI     StuI     StyI    TaqII
     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AlfI    AlwNI     ApaI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI     BbsI    BciVI     BclI    BfrBI     BglI
     BlpI    BmgBI     BmrI     BmtI     BsaI    BsaAI    BsaWI    BseRI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TatI     TstI  Tth111I     XbaI     XmaI
     XmnI     ZraI