Restriction map for
pF1KE0758
(Linear) (Six-base) MAPSORT of: KIEE0758.seq Check: 6525 from: 1 to: 1296
KIEE0758.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 708 792 960 1212 1266 1296
Size: 708 84 168 252 54 30
Fragments arranged by size:
708 252 168 84 54 30
AflIII A'CryG_T
Cuts at: 0 750 1296
Size: 750 546
ApoI r'AATT_y
Cuts at: 0 590 674 758 1178 1296
Size: 590 84 84 420 118
Fragments arranged by size:
590 420 118 84 84
AseI AT'TA_AT
Cuts at: 0 389 1296
Size: 389 907
BbsI GAAGACnn'nnnn_
Cuts at: 0 418 1296
Size: 418 878
BmrI ACTGGGnnnn_n'
Cuts at: 0 60 1296
Size: 60 1236
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 1040 1124 1208 1296
Size: 25 1015 84 84 88
Fragments arranged by size:
1015 88 84 84 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 30 1296
Size: 30 1266
BsmI GAATG_Cn'
Cuts at: 0 1126 1296
Size: 1126 170
BsrDI GCAATG_nn'
Cuts at: 0 1293 1296
Size: 1293 3
BstXI CCAn_nnnn'nTGG
Cuts at: 0 820 1296
Size: 820 476
Bsu36I CC'TnA_GG
Cuts at: 0 910 1296
Size: 910 386
BtgI C'CryG_G
Cuts at: 0 88 1296
Size: 88 1208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 722 1296
Size: 722 574
BtsI GCAGTG_nn'
Cuts at: 0 1254 1296
Size: 1254 42
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 861 896 1296
Size: 861 35 400
Fragments arranged by size:
861 400 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 422 1296
Size: 422 874
EarI CTCTTCn'nnn_
Cuts at: 0 130 1296
Size: 130 1166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 708 792 960 1040 1124 1208 1212
Size: 25 683 84 168 80 84 84 4
Cuts at: 1212 1266 1296
Size: 54 30
Fragments arranged by size:
683 168 84 84 84 80 54 30
25 4
EcoRI G'AATT_C
Cuts at: 0 590 674 758 1178 1296
Size: 590 84 84 420 118
Fragments arranged by size:
590 420 118 84 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1059 1091 1296
Size: 1059 32 205
Fragments arranged by size:
1059 205 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 1296
Size: 50 1246
MslI CAynn'nnrTG
Cuts at: 0 683 935 1035 1296
Size: 683 252 100 261
Fragments arranged by size:
683 261 252 100
MspA1I CmG'CkG
Cuts at: 0 1232 1296
Size: 1232 64
NcoI C'CATG_G
Cuts at: 0 88 1296
Size: 88 1208
NspI r_CATG'y
Cuts at: 0 240 754 872 1296
Size: 240 514 118 424
Fragments arranged by size:
514 424 240 118
PciI A'CATG_T
Cuts at: 0 750 1296
Size: 750 546
PflMI CCAn_nnn'nTGG
Cuts at: 0 303 1296
Size: 303 993
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 1296
Size: 10 32 1254
Fragments arranged by size:
1254 32 10
PvuII CAG'CTG
Cuts at: 0 1232 1296
Size: 1232 64
SapI GCTCTTCn'nnn_
Cuts at: 0 130 1296
Size: 130 1166
SexAI A'CCwGG_T
Cuts at: 0 20 1296
Size: 20 1276
SfcI C'TryA_G
Cuts at: 0 1233 1296
Size: 1233 63
SspI AAT'ATT
Cuts at: 0 540 1296
Size: 540 756
StyI C'CwwG_G
Cuts at: 0 88 248 1296
Size: 88 160 1048
Fragments arranged by size:
1048 160 88
TatI w'GTAC_w
Cuts at: 0 985 1296
Size: 985 311
XmnI GAAnn'nnTTC
Cuts at: 0 340 610 721 805 1296
Size: 340 270 111 84 491
Fragments arranged by size:
491 340 270 111 84
Enzymes that do cut:
AcuI AflIII ApoI AseI BbsI BmrI BpmI BsaI
BsmI BsrDI BstXI Bsu36I BtgI BtgZI BtsI CspCI
DrdI EarI Eco57MI EcoRI Hin4I MmeI MslI MspA1I
NcoI NspI PciI PflMI PpiI PvuII SapI SexAI
SfcI SspI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
ClaI DraI DraIII EaeI EagI EciI EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI ZraI