Restriction map for
pF1KE0764
(Linear) (Six-base) MAPSORT of: KIEE0764.seq Check: 754 from: 1 to: 1311
KIEE0764.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AclI AA'CG_TT
Cuts at: 0 715 1311
Size: 715 596
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 713 944 1311
Size: 226 487 231 367
Fragments arranged by size:
487 367 231 226
AflIII A'CryG_T
Cuts at: 0 386 1311
Size: 386 925
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 318 1311
Size: 318 993
AlwNI CAG_nnn'CTG
Cuts at: 0 1137 1311
Size: 1137 174
ApoI r'AATT_y
Cuts at: 0 1302 1311
Size: 1302 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 619 1311
Size: 619 692
BglII A'GATC_T
Cuts at: 0 111 1311
Size: 111 1200
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 83 1311
Size: 83 1228
Bpu10I CC'TnA_GC
Cuts at: 0 172 1311
Size: 172 1139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 1255 1311
Size: 53 1202 56
Fragments arranged by size:
1202 56 53
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 931 961 1311
Size: 931 30 350
Fragments arranged by size:
931 350 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 108 497 968 1311
Size: 108 389 471 343
Fragments arranged by size:
471 389 343 108
BseYI C'CCAG_C
Cuts at: 0 562 619 1311
Size: 562 57 692
Fragments arranged by size:
692 562 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 521 1311
Size: 394 127 790
Fragments arranged by size:
790 394 127
BsiHKAI G_wGCw'C
Cuts at: 0 33 1311
Size: 33 1278
BsmI GAATG_Cn'
Cuts at: 0 307 1311
Size: 307 1004
Bsp1286I G_dGCh'C
Cuts at: 0 33 1311
Size: 33 1278
BspHI T'CATG_A
Cuts at: 0 852 1184 1311
Size: 852 332 127
Fragments arranged by size:
852 332 127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 278 1311
Size: 278 1033
BsrGI T'GTAC_A
Cuts at: 0 947 1311
Size: 947 364
BstYI r'GATC_y
Cuts at: 0 111 474 1311
Size: 111 363 837
Fragments arranged by size:
837 363 111
BtsI GCAGTG_nn'
Cuts at: 0 33 1311
Size: 33 1278
DrdI GACnn_nn'nnGTC
Cuts at: 0 918 1311
Size: 918 393
EaeI y'GGCC_r
Cuts at: 0 1118 1311
Size: 1118 193
EarI CTCTTCn'nnn_
Cuts at: 0 597 902 1311
Size: 597 305 409
Fragments arranged by size:
597 409 305
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 713 944 1311
Size: 226 487 231 367
Fragments arranged by size:
487 367 231 226
EcoO109I rG'GnC_Cy
Cuts at: 0 768 1311
Size: 768 543
EcoRV GAT'ATC
Cuts at: 0 101 1311
Size: 101 1210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 318 350 640 672 834 866 878 910
Size: 318 32 290 32 162 32 12 32
Cuts at: 910 1311
Size: 401
Fragments arranged by size:
401 318 290 162 32 32 32 32
12
HincII GTy'rAC
Cuts at: 0 267 675 1311
Size: 267 408 636
Fragments arranged by size:
636 408 267
HpaI GTT'AAC
Cuts at: 0 675 1311
Size: 675 636
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 1311
Size: 70 1241
MscI TGG'CCA
Cuts at: 0 1120 1311
Size: 1120 191
MslI CAynn'nnrTG
Cuts at: 0 300 1098 1311
Size: 300 798 213
Fragments arranged by size:
798 300 213
MspA1I CmG'CkG
Cuts at: 0 364 1224 1311
Size: 364 860 87
Fragments arranged by size:
860 364 87
NspI r_CATG'y
Cuts at: 0 319 1311
Size: 319 992
PflMI CCAn_nnn'nTGG
Cuts at: 0 1137 1311
Size: 1137 174
PfoI T'CCnGG_A
Cuts at: 0 1202 1311
Size: 1202 109
PvuII CAG'CTG
Cuts at: 0 364 1311
Size: 364 947
SmlI C'TyrA_G
Cuts at: 0 68 1270 1311
Size: 68 1202 41
Fragments arranged by size:
1202 68 41
SphI G_CATG'C
Cuts at: 0 319 1311
Size: 319 992
StyI C'CwwG_G
Cuts at: 0 132 1311
Size: 132 1179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 186 576 633 1004 1311
Size: 186 390 57 371 307
Fragments arranged by size:
390 371 307 186 57
TatI w'GTAC_w
Cuts at: 0 221 947 1311
Size: 221 726 364
Fragments arranged by size:
726 364 221
Tth111I GACn'n_nGTC
Cuts at: 0 525 1311
Size: 525 786
XmnI GAAnn'nnTTC
Cuts at: 0 190 1311
Size: 190 1121
Enzymes that do cut:
AclI AcuI AflIII AlfI AlwNI ApoI BbsI BglII
BplI Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrGI BstYI BtsI DrdI
EaeI EarI Eco57MI EcoO109I EcoRV Hin4I HincII HpaI
MmeI MscI MslI MspA1I NspI PflMI PfoI PvuII
SmlI SphI StyI TaqII TatI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BmtI BpmI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI
EcoRI FalI FseI FspI FspAI HaeII HindIII KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI TstI
XbaI XcmI XhoI XmaI ZraI