(Linear) (Six-base) MAPSORT of: KIEE0777.seq  Check: 3214  from: 1  to: 993



KIEE0777.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     249     993 
   Size:        249     744
 
AlwNI CAG_nnn'CTG

Cuts at:      0     700     993 
   Size:        700     293
 
ApoI r'AATT_y

Cuts at:      0     756     993 
   Size:        756     237
 
BglII A'GATC_T

Cuts at:      0     260     993 
   Size:        260     733
 
Bme1580I G_kGCm'C

Cuts at:      0     248     993 
   Size:        248     745
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     788     993 
   Size:        788     205
 
BseYI C'CCAG_C

Cuts at:      0     318     379     993 
   Size:        318      61     614

  Fragments arranged by size: 

                 614     318      61
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     993 
   Size:        205     788
 
BsiEI CG_ry'CG

Cuts at:      0     331     993 
   Size:        331     662
 
Bsp1286I G_dGCh'C

Cuts at:      0     248     993 
   Size:        248     745
 
BsrDI GCAATG_nn'

Cuts at:      0     584     993 
   Size:        584     409
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     214     700     993 
   Size:        214     486     293

  Fragments arranged by size: 

                 486     293     214
 
BstYI r'GATC_y

Cuts at:      0     260     993 
   Size:        260     733
 
BtgI C'CryG_G

Cuts at:      0     921     993 
   Size:        921      72
 
BtsI GCAGTG_nn'

Cuts at:      0     942     993 
   Size:        942      51
 
ClaI AT'CG_AT

Cuts at:      0     735     993 
   Size:        735     258
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     861     896     993 
   Size:        861      35      97

  Fragments arranged by size: 

                 861      97      35
 
EaeI y'GGCC_r

Cuts at:      0     918     993 
   Size:        918      75
 
EarI CTCTTCn'nnn_

Cuts at:      0     354     468     754     807     993 
   Size:        354     114     286      53     186

  Fragments arranged by size: 

                 354     286     186     114      53
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     249     788     993 
   Size:        249     539     205

  Fragments arranged by size: 

                 539     249     205
 
EcoRI G'AATT_C

Cuts at:      0     756     993 
   Size:        756     237
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     831     993 
   Size:         95     736     162

  Fragments arranged by size: 

                 736     162      95
 
MslI CAynn'nnrTG

Cuts at:      0     494     689     993 
   Size:        494     195     304

  Fragments arranged by size: 

                 494     304     195
 
NdeI CA'TA_TG

Cuts at:      0     339     548     993 
   Size:        339     209     445

  Fragments arranged by size: 

                 445     339     209
 
NspI r_CATG'y

Cuts at:      0     729     993 
   Size:        729     264
 
PfoI T'CCnGG_A

Cuts at:      0     765     993 
   Size:        765     228
 
StuI AGG'CCT

Cuts at:      0     178     993 
   Size:        178     815
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     898     993 
   Size:        898      95
 
XmnI GAAnn'nnTTC

Cuts at:      0      95     993 
   Size:         95     898
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI    BglII Bme1580I     BpmI    BseYI     BsgI
    BsiEI Bsp1286I    BsrDI   BstAPI    BstYI     BtgI     BtsI     ClaI
    CspCI     EaeI     EarI  Eco57MI    EcoRI     MmeI     MslI     NdeI
     NspI     PfoI     StuI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI