Restriction map for
pF1KE0778
(Linear) (Six-base) MAPSORT of: KIEE0778.seq Check: 5023 from: 1 to: 1542
KIEE0778.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 0 1345 1542
Size: 1345 197
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 896 1283 1304 1542
Size: 896 387 21 238
Fragments arranged by size:
896 387 238 21
AflIII A'CryG_T
Cuts at: 0 847 941 1542
Size: 847 94 601
Fragments arranged by size:
847 601 94
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1385 1542
Size: 1385 157
ApoI r'AATT_y
Cuts at: 0 605 1041 1542
Size: 605 436 501
Fragments arranged by size:
605 501 436
BanI G'GyrC_C
Cuts at: 0 1513 1542
Size: 1513 29
BbeI G_GCGC'C
Cuts at: 0 1517 1542
Size: 1517 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 912 946 1542
Size: 912 34 596
Fragments arranged by size:
912 596 34
BglII A'GATC_T
Cuts at: 0 711 1542
Size: 711 831
BlpI GC'TnA_GC
Cuts at: 0 801 1542
Size: 801 741
Bme1580I G_kGCm'C
Cuts at: 0 891 1542
Size: 891 651
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 190 195 1542
Size: 89 101 5 1347
Fragments arranged by size:
1347 101 89 5
Bpu10I CC'TnA_GC
Cuts at: 0 1115 1542
Size: 1115 427
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 1542
Size: 213 1329
BsaI GGTCTCn'nnnn_
Cuts at: 0 1161 1542
Size: 1161 381
BsaAI yAC'GTr
Cuts at: 0 952 1542
Size: 952 590
BsaHI Gr'CG_yC
Cuts at: 0 929 1514 1542
Size: 929 585 28
Fragments arranged by size:
929 585 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1345 1542
Size: 1345 197
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 1419 1542
Size: 170 1249 123
Fragments arranged by size:
1249 170 123
Bsp1286I G_dGCh'C
Cuts at: 0 891 1542
Size: 891 651
BsrGI T'GTAC_A
Cuts at: 0 702 938 1542
Size: 702 236 604
Fragments arranged by size:
702 604 236
BstYI r'GATC_y
Cuts at: 0 711 1092 1542
Size: 711 381 450
Fragments arranged by size:
711 450 381
BtsI GCAGTG_nn'
Cuts at: 0 913 1542
Size: 913 629
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 950 985 1542
Size: 950 35 557
Fragments arranged by size:
950 557 35
EarI CTCTTCn'nnn_
Cuts at: 0 977 1235 1322 1542
Size: 977 258 87 220
Fragments arranged by size:
977 258 220 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 190 195 896 1283 1304 1542
Size: 89 101 5 701 387 21 238
Fragments arranged by size:
701 387 238 101 89 21 5
EcoRI G'AATT_C
Cuts at: 0 1041 1542
Size: 1041 501
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 88 1006 1404 1542
Size: 88 918 398 138
Fragments arranged by size:
918 398 138 88
HaeII r_GCGC'y
Cuts at: 0 1517 1542
Size: 1517 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 34 42 66 193 211 225 243
Size: 10 24 8 24 127 18 14 18
Cuts at: 243 1542
Size: 1299
Fragments arranged by size:
1299 127 24 24 18 18 14 10
8
HincII GTy'rAC
Cuts at: 0 1142 1542
Size: 1142 400
HindIII A'AGCT_T
Cuts at: 0 988 1542
Size: 988 554
HpaI GTT'AAC
Cuts at: 0 1142 1542
Size: 1142 400
KasI G'GCGC_C
Cuts at: 0 1513 1542
Size: 1513 29
MslI CAynn'nnrTG
Cuts at: 0 692 784 1542
Size: 692 92 758
Fragments arranged by size:
758 692 92
MspA1I CmG'CkG
Cuts at: 0 1435 1542
Size: 1435 107
NarI GG'CG_CC
Cuts at: 0 1514 1542
Size: 1514 28
NspI r_CATG'y
Cuts at: 0 851 945 1542
Size: 851 94 597
Fragments arranged by size:
851 597 94
PasI CC'CwG_GG
Cuts at: 0 1289 1542
Size: 1289 253
PciI A'CATG_T
Cuts at: 0 847 941 1542
Size: 847 94 601
Fragments arranged by size:
847 601 94
PfoI T'CCnGG_A
Cuts at: 0 211 1542
Size: 211 1331
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 509 541 1542
Size: 509 32 1001
Fragments arranged by size:
1001 509 32
PstI C_TGCA'G
Cuts at: 0 842 1542
Size: 842 700
PvuII CAG'CTG
Cuts at: 0 1435 1542
Size: 1435 107
SfcI C'TryA_G
Cuts at: 0 674 838 897 1542
Size: 674 164 59 645
Fragments arranged by size:
674 645 164 59
SfoI GGC'GCC
Cuts at: 0 1515 1542
Size: 1515 27
SmlI C'TyrA_G
Cuts at: 0 228 1542
Size: 228 1314
SspI AAT'ATT
Cuts at: 0 362 1542
Size: 362 1180
StyI C'CwwG_G
Cuts at: 0 1479 1542
Size: 1479 63
TatI w'GTAC_w
Cuts at: 0 69 702 938 1542
Size: 69 633 236 604
Fragments arranged by size:
633 604 236 69
Enzymes that do cut:
AccI AcuI AflIII AlfI ApoI BanI BbeI BcgI
BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BseRI BsgI Bsp1286I BsrGI BstYI BtsI CspCI
EarI Eco57MI EcoRI FalI HaeII Hin4I HincII HindIII
HpaI KasI MslI MspA1I NarI NspI PasI PciI
PfoI PsrI PstI PvuII SfcI SfoI SmlI SspI
StyI TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbsI BbvCI BciVI
BclI BfrBI BglI BmgBI BmrI BmtI BplI BsaBI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI DraI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI EcoO109I EcoRV FseI FspI FspAI KpnI MfeI
MluI MmeI MscI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI