Restriction map for
pF1KE0779
(Linear) (Six-base) MAPSORT of: KIEE0779.seq Check: 2732 from: 1 to: 1044
KIEE0779.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 571 1044
Size: 289 282 473
Fragments arranged by size:
473 289 282
AfeI AGC'GCT
Cuts at: 0 948 1044
Size: 948 96
AlwNI CAG_nnn'CTG
Cuts at: 0 156 1044
Size: 156 888
ApaLI G'TGCA_C
Cuts at: 0 478 1044
Size: 478 566
AvaI C'yCGr_G
Cuts at: 0 676 703 857 1044
Size: 676 27 154 187
Fragments arranged by size:
676 187 154 27
BanI G'GyrC_C
Cuts at: 0 977 1044
Size: 977 67
BanII G_rGCy'C
Cuts at: 0 95 182 332 416 683 1044
Size: 95 87 150 84 267 361
Fragments arranged by size:
361 267 150 95 87 84
BbeI G_GCGC'C
Cuts at: 0 981 1044
Size: 981 63
BbvCI CC'TCA_GC
Cuts at: 0 869 1044
Size: 869 175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 26 60 360 394 1044
Size: 26 34 300 34 650
Fragments arranged by size:
650 300 34 34 26
BglI GCCn_nnn'nGGC
Cuts at: 0 499 895 1044
Size: 499 396 149
Fragments arranged by size:
499 396 149
Bme1580I G_kGCm'C
Cuts at: 0 482 1044
Size: 482 562
BmgBI CAC'GTC
Cuts at: 0 293 1044
Size: 293 751
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 145 532 1005 1044
Size: 79 66 387 473 39
Fragments arranged by size:
473 387 79 66 39
Bpu10I CC'TnA_GC
Cuts at: 0 869 1044
Size: 869 175
BsaBI GATnn'nnATC
Cuts at: 0 958 1044
Size: 958 86
BsaHI Gr'CG_yC
Cuts at: 0 734 749 844 978 1044
Size: 734 15 95 134 66
Fragments arranged by size:
734 134 95 66 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 76 103 757 957 1044
Size: 73 3 27 654 200 87
Fragments arranged by size:
654 200 87 73 27 3
BseYI C'CCAG_C
Cuts at: 0 336 494 744 1036 1044
Size: 336 158 250 292 8
Fragments arranged by size:
336 292 250 158 8
BsiEI CG_ry'CG
Cuts at: 0 899 1044
Size: 899 145
BsiHKAI G_wGCw'C
Cuts at: 0 95 150 182 482 683 971 1044
Size: 95 55 32 300 201 288 73
Fragments arranged by size:
300 288 201 95 73 55 32
Bsp1286I G_dGCh'C
Cuts at: 0 95 150 182 332 416 482 683 971
Size: 95 55 32 150 84 66 201 288
Cuts at: 971 1044
Size: 73
Fragments arranged by size:
288 201 150 95 84 73 66 55
32
BsrBI CCG'CTC
Cuts at: 0 699 1021 1044
Size: 699 322 23
Fragments arranged by size:
699 322 23
BsrFI r'CCGG_y
Cuts at: 0 27 587 791 1044
Size: 27 560 204 253
Fragments arranged by size:
560 253 204 27
BssHII G'CGCG_C
Cuts at: 0 119 341 686 1044
Size: 119 222 345 358
Fragments arranged by size:
358 345 222 119
BssSI C'ACGA_G
Cuts at: 0 618 1044
Size: 618 426
BstEII G'GTnAC_C
Cuts at: 0 234 1044
Size: 234 810
BtgI C'CryG_G
Cuts at: 0 300 473 528 893 1044
Size: 300 173 55 365 151
Fragments arranged by size:
365 300 173 151 55
BtsI GCAGTG_nn'
Cuts at: 0 1019 1044
Size: 1019 25
EaeI y'GGCC_r
Cuts at: 0 219 612 647 800 896 1044
Size: 219 393 35 153 96 148
Fragments arranged by size:
393 219 153 148 96 35
EagI C'GGCC_G
Cuts at: 0 896 1044
Size: 896 148
EarI CTCTTCn'nnn_
Cuts at: 0 763 1022 1044
Size: 763 259 22
Fragments arranged by size:
763 259 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 46 495 1021 1044
Size: 46 449 526 23
Fragments arranged by size:
526 449 46 23
EcoICRI GAG'CTC
Cuts at: 0 93 180 681 1044
Size: 93 87 501 363
Fragments arranged by size:
501 363 93 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 145 289 532 571 1005 1044
Size: 79 66 144 243 39 434 39
Fragments arranged by size:
434 243 144 79 66 39 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 205 802 1044
Size: 205 597 242
Fragments arranged by size:
597 242 205
EcoO109I rG'GnC_Cy
Cuts at: 0 429 546 708 1044
Size: 429 117 162 336
Fragments arranged by size:
429 336 162 117
HaeII r_GCGC'y
Cuts at: 0 594 745 950 981 1044
Size: 594 151 205 31 63
Fragments arranged by size:
594 205 151 63 31
KasI G'GCGC_C
Cuts at: 0 977 1044
Size: 977 67
MspA1I CmG'CkG
Cuts at: 0 475 485 498 530 895 993 1044
Size: 475 10 13 32 365 98 51
Fragments arranged by size:
475 365 98 51 32 13 10
NaeI GCC'GGC
Cuts at: 0 29 589 1044
Size: 29 560 455
Fragments arranged by size:
560 455 29
NarI GG'CG_CC
Cuts at: 0 978 1044
Size: 978 66
NgoMIV G'CCGG_C
Cuts at: 0 27 587 1044
Size: 27 560 457
Fragments arranged by size:
560 457 27
PasI CC'CwG_GG
Cuts at: 0 201 785 1044
Size: 201 584 259
Fragments arranged by size:
584 259 201
PpuMI rG'GwC_Cy
Cuts at: 0 429 546 1044
Size: 429 117 498
Fragments arranged by size:
498 429 117
PshAI GACnn'nnGTC
Cuts at: 0 157 1044
Size: 157 887
PvuII CAG'CTG
Cuts at: 0 498 1044
Size: 498 546
SacI G_AGCT'C
Cuts at: 0 95 182 683 1044
Size: 95 87 501 361
Fragments arranged by size:
501 361 95 87
SacII CC_GC'GG
Cuts at: 0 476 531 896 1044
Size: 476 55 365 148
Fragments arranged by size:
476 365 148 55
SapI GCTCTTCn'nnn_
Cuts at: 0 763 1044
Size: 763 281
SfcI C'TryA_G
Cuts at: 0 396 1044
Size: 396 648
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 895 1044
Size: 895 149
SfoI GGC'GCC
Cuts at: 0 979 1044
Size: 979 65
SmaI CCC'GGG
Cuts at: 0 859 1044
Size: 859 185
StuI AGG'CCT
Cuts at: 0 624 1044
Size: 624 420
StyI C'CwwG_G
Cuts at: 0 452 1044
Size: 452 592
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 253 619 1044
Size: 253 366 425
Fragments arranged by size:
425 366 253
TatI w'GTAC_w
Cuts at: 0 515 1044
Size: 515 529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 214 1044
Size: 214 830
XmaI C'CCGG_G
Cuts at: 0 857 1044
Size: 857 187
Enzymes that do cut:
AcuI AfeI AlwNI ApaLI AvaI BanI BanII BbeI
BbvCI BcgI BglI Bme1580I BmgBI BpmI Bpu10I BsaBI
BsaHI BseRI BseYI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI
BssHII BssSI BstEII BtgI BtsI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII KasI MspA1I
NaeI NarI NgoMIV PasI PpuMI PshAI PvuII SacI
SacII SapI SfcI SfiI SfoI SmaI StuI StyI
TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI
BglII BlpI BmrI BmtI BplI BpuEI BsaI BsaAI
BsaWI BsaXI BsgI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrDI BsrGI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SalI SanDI SbfI ScaI
SexAI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XhoI XmnI ZraI