Restriction map for
pF1KE0782
(Linear) (Six-base) MAPSORT of: KIEE0782.seq Check: 8164 from: 1 to: 1359
KIEE0782.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1135 1317 1359
Size: 1135 182 42
Fragments arranged by size:
1135 182 42
AfeI AGC'GCT
Cuts at: 0 14 1063 1359
Size: 14 1049 296
Fragments arranged by size:
1049 296 14
AflIII A'CryG_T
Cuts at: 0 89 296 1359
Size: 89 207 1063
Fragments arranged by size:
1063 207 89
AhdI GACnn_n'nnGTC
Cuts at: 0 672 1359
Size: 672 687
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 305 1096 1187 1359
Size: 305 791 91 172
Fragments arranged by size:
791 305 172 91
ApoI r'AATT_y
Cuts at: 0 1154 1179 1359
Size: 1154 25 180
Fragments arranged by size:
1154 180 25
AvaI C'yCGr_G
Cuts at: 0 50 597 1254 1359
Size: 50 547 657 105
Fragments arranged by size:
657 547 105 50
BanI G'GyrC_C
Cuts at: 0 66 271 370 1359
Size: 66 205 99 989
Fragments arranged by size:
989 205 99 66
BanII G_rGCy'C
Cuts at: 0 59 182 476 1359
Size: 59 123 294 883
Fragments arranged by size:
883 294 123 59
BbeI G_GCGC'C
Cuts at: 0 70 374 1359
Size: 70 304 985
Fragments arranged by size:
985 304 70
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 768 802 1245 1279 1359
Size: 768 34 443 34 80
Fragments arranged by size:
768 443 80 34 34
BclI T'GATC_A
Cuts at: 0 554 1359
Size: 554 805
BglI GCCn_nnn'nGGC
Cuts at: 0 31 40 122 1359
Size: 31 9 82 1237
Fragments arranged by size:
1237 82 31 9
BlpI GC'TnA_GC
Cuts at: 0 397 1359
Size: 397 962
Bme1580I G_kGCm'C
Cuts at: 0 134 284 512 1359
Size: 134 150 228 847
Fragments arranged by size:
847 228 150 134
BmgBI CAC'GTC
Cuts at: 0 90 435 1359
Size: 90 345 924
Fragments arranged by size:
924 345 90
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 34 1359
Size: 34 1325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 1321 1359
Size: 241 1080 38
Fragments arranged by size:
1080 241 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 949 1359
Size: 598 351 410
Fragments arranged by size:
598 410 351
BsaI GGTCTCn'nnnn_
Cuts at: 0 391 1359
Size: 391 968
BsaAI yAC'GTr
Cuts at: 0 129 573 1359
Size: 129 444 786
Fragments arranged by size:
786 444 129
BsaHI Gr'CG_yC
Cuts at: 0 67 346 371 1239 1359
Size: 67 279 25 868 120
Fragments arranged by size:
868 279 120 67 25
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 895 925 1272 1302 1359
Size: 895 30 347 30 57
Fragments arranged by size:
895 347 57 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1280 1318 1359
Size: 1280 38 41
Fragments arranged by size:
1280 41 38
BseYI C'CCAG_C
Cuts at: 0 103 729 830 849 1359
Size: 103 626 101 19 510
Fragments arranged by size:
626 510 103 101 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1359
Size: 640 719
BsiEI CG_ry'CG
Cuts at: 0 743 1359
Size: 743 616
BsiHKAI G_wGCw'C
Cuts at: 0 476 1359
Size: 476 883
Bsp1286I G_dGCh'C
Cuts at: 0 59 134 182 284 476 512 1359
Size: 59 75 48 102 192 36 847
Fragments arranged by size:
847 192 102 75 59 48 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 755 1359
Size: 755 604
BsrDI GCAATG_nn'
Cuts at: 0 425 680 1359
Size: 425 255 679
Fragments arranged by size:
679 425 255
BsrFI r'CCGG_y
Cuts at: 0 547 1359
Size: 547 812
BssHII G'CGCG_C
Cuts at: 0 79 302 326 1359
Size: 79 223 24 1033
Fragments arranged by size:
1033 223 79 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 149 1359
Size: 149 1210
BstYI r'GATC_y
Cuts at: 0 695 1359
Size: 695 664
Bsu36I CC'TnA_GG
Cuts at: 0 1346 1359
Size: 1346 13
BtgI C'CryG_G
Cuts at: 0 458 1359
Size: 458 901
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1323 1359
Size: 1323 36
BtsI GCAGTG_nn'
Cuts at: 0 824 1155 1359
Size: 824 331 204
Fragments arranged by size:
824 331 204
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1085 1120 1359
Size: 1085 35 239
Fragments arranged by size:
1085 239 35
DraIII CAC_nnn'GTG
Cuts at: 0 264 576 1359
Size: 264 312 783
Fragments arranged by size:
783 312 264
DrdI GACnn_nn'nnGTC
Cuts at: 0 1222 1359
Size: 1222 137
EaeI y'GGCC_r
Cuts at: 0 23 32 314 498 740 770 1359
Size: 23 9 282 184 242 30 589
Fragments arranged by size:
589 282 242 184 30 23 9
EagI C'GGCC_G
Cuts at: 0 740 1359
Size: 740 619
EarI CTCTTCn'nnn_
Cuts at: 0 481 1336 1359
Size: 481 855 23
Fragments arranged by size:
855 481 23
EcoICRI GAG'CTC
Cuts at: 0 474 1359
Size: 474 885
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 1135 1317 1321 1359
Size: 241 894 182 4 38
Fragments arranged by size:
894 241 182 38 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1300 1359
Size: 1300 59
EcoRI G'AATT_C
Cuts at: 0 1179 1359
Size: 1179 180
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 438 1080 1359
Size: 438 642 279
Fragments arranged by size:
642 438 279
FseI GG_CCGG'CC
Cuts at: 0 551 1359
Size: 551 808
HaeII r_GCGC'y
Cuts at: 0 16 46 70 374 1065 1359
Size: 16 30 24 304 691 294
Fragments arranged by size:
691 304 294 30 24 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 658 690 1187 1219 1272 1304 1359
Size: 658 32 497 32 53 32 55
Fragments arranged by size:
658 497 55 53 32 32 32
HincII GTy'rAC
Cuts at: 0 1233 1359
Size: 1233 126
KasI G'GCGC_C
Cuts at: 0 66 370 1359
Size: 66 304 989
Fragments arranged by size:
989 304 66
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 890 1359
Size: 656 234 469
Fragments arranged by size:
656 469 234
MscI TGG'CCA
Cuts at: 0 772 1359
Size: 772 587
MslI CAynn'nnrTG
Cuts at: 0 287 1078 1359
Size: 287 791 281
Fragments arranged by size:
791 287 281
MspA1I CmG'CkG
Cuts at: 0 113 245 251 360 396 492 521 834
Size: 113 132 6 109 36 96 29 313
Cuts at: 834 1359
Size: 525
Fragments arranged by size:
525 313 132 113 109 96 36 29
6
NaeI GCC'GGC
Cuts at: 0 549 1359
Size: 549 810
NarI GG'CG_CC
Cuts at: 0 67 371 1359
Size: 67 304 988
Fragments arranged by size:
988 304 67
NgoMIV G'CCGG_C
Cuts at: 0 547 1359
Size: 547 812
PfoI T'CCnGG_A
Cuts at: 0 340 857 1359
Size: 340 517 502
Fragments arranged by size:
517 502 340
PmlI CAC'GTG
Cuts at: 0 573 1359
Size: 573 786
PspXI vC'TCGA_Gb
Cuts at: 0 1254 1359
Size: 1254 105
PstI C_TGCA'G
Cuts at: 0 311 335 365 1268 1359
Size: 311 24 30 903 91
Fragments arranged by size:
903 311 91 30 24
PvuII CAG'CTG
Cuts at: 0 360 396 1359
Size: 360 36 963
Fragments arranged by size:
963 360 36
SacI G_AGCT'C
Cuts at: 0 476 1359
Size: 476 883
SapI GCTCTTCn'nnn_
Cuts at: 0 481 1359
Size: 481 878
SfcI C'TryA_G
Cuts at: 0 307 331 361 625 793 1264 1359
Size: 307 24 30 264 168 471 95
Fragments arranged by size:
471 307 264 168 95 30 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 31 1359
Size: 31 1328
SfoI GGC'GCC
Cuts at: 0 68 372 1359
Size: 68 304 987
Fragments arranged by size:
987 304 68
SmaI CCC'GGG
Cuts at: 0 52 599 1359
Size: 52 547 760
Fragments arranged by size:
760 547 52
SmlI C'TyrA_G
Cuts at: 0 613 964 1254 1359
Size: 613 351 290 105
Fragments arranged by size:
613 351 290 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1297 1359
Size: 1297 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 562 594 911 943 1145 1177 1359
Size: 562 32 317 32 202 32 182
Fragments arranged by size:
562 317 202 182 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1284 1359
Size: 1284 75
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 570 1359
Size: 570 789
XhoI C'TCGA_G
Cuts at: 0 1254 1359
Size: 1254 105
XmaI C'CCGG_G
Cuts at: 0 50 597 1359
Size: 50 547 762
Fragments arranged by size:
762 547 50
XmnI GAAnn'nnTTC
Cuts at: 0 1063 1359
Size: 1063 296
Enzymes that do cut:
AcuI AfeI AflIII AhdI AlfI ApoI AvaI BanI
BanII BbeI BcgI BclI BglI BlpI Bme1580I BmgBI
BplI BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI
BssHII BstAPI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoRI FalI FseI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV PfoI PmlI
PspXI PstI PvuII SacI SapI SfcI SfiI SfoI
SmaI SmlI TaqII TstI Tth111I XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AleI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BciVI BfrBI BglII BmrI
BmtI Bpu10I BsaBI BsaWI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrGI BssSI BstBI BstEII BstXI BstZ17I
ClaI DraI EciI EcoO109I EcoRV FspI FspAI HindIII
HpaI KpnI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI XbaI ZraI