(Linear) (Six-base) MAPSORT of: KIEE0784.seq  Check: 1668  from: 1  to: 1269



KIEE0784.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:31  ..

AccI GT'mk_AC

Cuts at:      0     294    1269 
   Size:        294     975
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1187    1269 
   Size:       1187      82
 
AflII C'TTAA_G

Cuts at:      0     909    1269 
   Size:        909     360
 
AflIII A'CryG_T

Cuts at:      0    1211    1269 
   Size:       1211      58
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1037    1269 
   Size:       1037     232
 
ApoI r'AATT_y

Cuts at:      0     183     834    1269 
   Size:        183     651     435

  Fragments arranged by size: 

                 651     435     183
 
AvaI C'yCGr_G

Cuts at:      0      77    1269 
   Size:         77    1192
 
BanII G_rGCy'C

Cuts at:      0     224    1269 
   Size:        224    1045
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      83     377     730    1269 
   Size:         83     294     353     539

  Fragments arranged by size: 

                 539     353     294      83
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      95    1269 
   Size:         95    1174
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     305     980    1269 
   Size:        122     183     675     289

  Fragments arranged by size: 

                 675     289     183     122
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     284    1269 
   Size:        284     985
 
BsaWI w'CCGG_w

Cuts at:      0     582    1269 
   Size:        582     687
 
BseYI C'CCAG_C

Cuts at:      0     354     530    1269 
   Size:        354     176     739

  Fragments arranged by size: 

                 739     354     176
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1025    1269 
   Size:       1025     244
 
BsiEI CG_ry'CG

Cuts at:      0      64    1269 
   Size:         64    1205
 
BsiHKAI G_wGCw'C

Cuts at:      0     240    1269 
   Size:        240    1029
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     680    1269 
   Size:        680     589
 
Bsp1286I G_dGCh'C

Cuts at:      0     224     240    1269 
   Size:        224      16    1029

  Fragments arranged by size: 

                1029     224      16
 
BspEI T'CCGG_A

Cuts at:      0     582    1269 
   Size:        582     687
 
BspHI T'CATG_A

Cuts at:      0     419    1269 
   Size:        419     850
 
BstYI r'GATC_y

Cuts at:      0     280    1017    1269 
   Size:        280     737     252

  Fragments arranged by size: 

                 737     280     252
 
BstZ17I GTA'TAC

Cuts at:      0     295    1269 
   Size:        295     974
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      56    1269 
   Size:         56    1213
 
EarI CTCTTCn'nnn_

Cuts at:      0     678    1269 
   Size:        678     591
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     305     980    1187    1269 
   Size:        122     183     675     207      82

  Fragments arranged by size: 

                 675     207     183     122      82
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     746    1269 
   Size:        746     523
 
EcoO109I rG'GnC_Cy

Cuts at:      0     358    1269 
   Size:        358     911
 
HaeII r_GCGC'y

Cuts at:      0    1263    1269 
   Size:       1263       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     720     752    1269 
   Size:        720      32     517

  Fragments arranged by size: 

                 720     517      32
 
HindIII A'AGCT_T

Cuts at:      0     698     916    1269 
   Size:        698     218     353

  Fragments arranged by size: 

                 698     353     218
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      96     114     197    1269 
   Size:         96      18      83    1072

  Fragments arranged by size: 

                1072      96      83      18
 
NdeI CA'TA_TG

Cuts at:      0     710    1269 
   Size:        710     559
 
NspI r_CATG'y

Cuts at:      0    1215    1269 
   Size:       1215      54
 
PciI A'CATG_T

Cuts at:      0    1211    1269 
   Size:       1211      58
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     387     419    1269 
   Size:        387      32     850

  Fragments arranged by size: 

                 850     387      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     358    1269 
   Size:        358     911
 
PspXI vC'TCGA_Gb

Cuts at:      0      77    1269 
   Size:         77    1192
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     460     492    1269 
   Size:        460      32     777

  Fragments arranged by size: 

                 777     460      32
 
PvuI CG_AT'CG

Cuts at:      0      64    1269 
   Size:         64    1205
 
SexAI A'CCwGG_T

Cuts at:      0    1082    1269 
   Size:       1082     187
 
SmlI C'TyrA_G

Cuts at:      0      77     299     909    1269 
   Size:         77     222     610     360

  Fragments arranged by size: 

                 610     360     222      77
 
StyI C'CwwG_G

Cuts at:      0     742    1269 
   Size:        742     527
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     991    1164    1269 
   Size:        991     173     105

  Fragments arranged by size: 

                 991     173     105
 
XhoI C'TCGA_G

Cuts at:      0      77    1269 
   Size:         77    1192
 
XmnI GAAnn'nnTTC

Cuts at:      0     399     836    1269 
   Size:        399     437     433

  Fragments arranged by size: 

                 437     433     399
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AlfI     ApoI     AvaI    BanII
     BbsI     BmrI     BpmI    BpuEI    BsaWI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BstYI  BstZ17I     DrdI
     EarI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I  HindIII     MmeI
     NdeI     NspI     PciI     PpiI    PpuMI    PspXI     PsrI     PvuI
    SexAI     SmlI     StyI    TaqII     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AleI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BsiWI
     BsmI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EciI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI    PshAI     PsiI   PspOMI
     PstI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI