Restriction map for
pF1KE0785
(Linear) (Six-base) MAPSORT of: KIEE0785.seq Check: 5671 from: 1 to: 1059
KIEE0785.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 93 618 1059
Size: 93 525 441
Fragments arranged by size:
525 441 93
Acc65I G'GTAC_C
Cuts at: 0 866 1042 1059
Size: 866 176 17
Fragments arranged by size:
866 176 17
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 679 885 1059
Size: 261 418 206 174
Fragments arranged by size:
418 261 206 174
AflIII A'CryG_T
Cuts at: 0 203 1059
Size: 203 856
AleI CACnn'nnGTG
Cuts at: 0 617 1059
Size: 617 442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 475 1059
Size: 475 584
AlwNI CAG_nnn'CTG
Cuts at: 0 480 783 1059
Size: 480 303 276
Fragments arranged by size:
480 303 276
ApoI r'AATT_y
Cuts at: 0 553 1059
Size: 553 506
AvaI C'yCGr_G
Cuts at: 0 318 532 764 1059
Size: 318 214 232 295
Fragments arranged by size:
318 295 232 214
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1034 1059
Size: 1034 25
BanI G'GyrC_C
Cuts at: 0 528 854 866 894 1042 1059
Size: 528 326 12 28 148 17
Fragments arranged by size:
528 326 148 28 17 12
BanII G_rGCy'C
Cuts at: 0 765 1059
Size: 765 294
BbeI G_GCGC'C
Cuts at: 0 532 1059
Size: 532 527
BbvCI CC'TCA_GC
Cuts at: 0 1034 1059
Size: 1034 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 652 686 1059
Size: 652 34 373
Fragments arranged by size:
652 373 34
BglI GCCn_nnn'nGGC
Cuts at: 0 589 1059
Size: 589 470
BlpI GC'TnA_GC
Cuts at: 0 418 1059
Size: 418 641
Bme1580I G_kGCm'C
Cuts at: 0 899 1059
Size: 899 160
BmgBI CAC'GTC
Cuts at: 0 204 1059
Size: 204 855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 1059
Size: 85 974
Bpu10I CC'TnA_GC
Cuts at: 0 1034 1059
Size: 1034 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 1059
Size: 631 428
BsaHI Gr'CG_yC
Cuts at: 0 529 1059
Size: 529 530
BsaWI w'CCGG_w
Cuts at: 0 891 1059
Size: 891 168
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 614 644 1059
Size: 614 30 415
Fragments arranged by size:
614 415 30
BseYI C'CCAG_C
Cuts at: 0 143 166 480 514 801 1027 1059
Size: 143 23 314 34 287 226 32
Fragments arranged by size:
314 287 226 143 34 32 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 337 1059
Size: 121 216 722
Fragments arranged by size:
722 216 121
BsiEI CG_ry'CG
Cuts at: 0 373 1059
Size: 373 686
BsiHKAI G_wGCw'C
Cuts at: 0 215 1059
Size: 215 844
Bsp1286I G_dGCh'C
Cuts at: 0 215 765 899 1059
Size: 215 550 134 160
Fragments arranged by size:
550 215 160 134
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 93 618 852 1030 1059
Size: 93 525 234 178 29
Fragments arranged by size:
525 234 178 93 29
BsrFI r'CCGG_y
Cuts at: 0 525 1059
Size: 525 534
BssHII G'CGCG_C
Cuts at: 0 329 455 1059
Size: 329 126 604
Fragments arranged by size:
604 329 126
BssSI C'ACGA_G
Cuts at: 0 388 1059
Size: 388 671
BstAPI GCAn_nnn'nTGC
Cuts at: 0 894 1059
Size: 894 165
BstXI CCAn_nnnn'nTGG
Cuts at: 0 831 1059
Size: 831 228
Bsu36I CC'TnA_GG
Cuts at: 0 197 1059
Size: 197 862
BtgI C'CryG_G
Cuts at: 0 291 578 1046 1059
Size: 291 287 468 13
Fragments arranged by size:
468 291 287 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 327 1059
Size: 327 732
DraIII CAC_nnn'GTG
Cuts at: 0 827 997 1059
Size: 827 170 62
Fragments arranged by size:
827 170 62
EaeI y'GGCC_r
Cuts at: 0 370 581 936 956 1059
Size: 370 211 355 20 103
Fragments arranged by size:
370 355 211 103 20
EagI C'GGCC_G
Cuts at: 0 370 1059
Size: 370 689
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 82 250 1059
Size: 82 168 809
Fragments arranged by size:
809 168 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 261 679 885 1059
Size: 85 176 418 206 174
Fragments arranged by size:
418 206 176 174 85
EcoNI CCTnn'n_nnAGG
Cuts at: 0 100 1059
Size: 100 959
EcoO109I rG'GnC_Cy
Cuts at: 0 484 591 688 841 1059
Size: 484 107 97 153 218
Fragments arranged by size:
484 218 153 107 97
HaeII r_GCGC'y
Cuts at: 0 64 532 1059
Size: 64 468 527
Fragments arranged by size:
527 468 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 59 955 987 1059
Size: 27 32 896 32 72
Fragments arranged by size:
896 72 32 32 27
HincII GTy'rAC
Cuts at: 0 426 1059
Size: 426 633
KasI G'GCGC_C
Cuts at: 0 528 1059
Size: 528 531
KpnI G_GTAC'C
Cuts at: 0 870 1046 1059
Size: 870 176 13
Fragments arranged by size:
870 176 13
MscI TGG'CCA
Cuts at: 0 583 958 1059
Size: 583 375 101
Fragments arranged by size:
583 375 101
MslI CAynn'nnrTG
Cuts at: 0 617 1059
Size: 617 442
MspA1I CmG'CkG
Cuts at: 0 166 228 281 293 366 384 480 574
Size: 166 62 53 12 73 18 96 94
Cuts at: 574 905 1059
Size: 331 154
Fragments arranged by size:
331 166 154 96 94 73 62 53
18 12
NarI GG'CG_CC
Cuts at: 0 529 1059
Size: 529 530
NcoI C'CATG_G
Cuts at: 0 578 1046 1059
Size: 578 468 13
Fragments arranged by size:
578 468 13
PflMI CCAn_nnn'nTGG
Cuts at: 0 240 1059
Size: 240 819
PpuMI rG'GwC_Cy
Cuts at: 0 484 688 841 1059
Size: 484 204 153 218
Fragments arranged by size:
484 218 204 153
PspXI vC'TCGA_Gb
Cuts at: 0 318 1059
Size: 318 741
PstI C_TGCA'G
Cuts at: 0 476 779 989 1059
Size: 476 303 210 70
Fragments arranged by size:
476 303 210 70
PvuII CAG'CTG
Cuts at: 0 166 1059
Size: 166 893
RsrII CG'GwC_CG
Cuts at: 0 523 1059
Size: 523 536
SacII CC_GC'GG
Cuts at: 0 294 1059
Size: 294 765
SanDI GG'GwC_CC
Cuts at: 0 484 688 1059
Size: 484 204 371
Fragments arranged by size:
484 371 204
SexAI A'CCwGG_T
Cuts at: 0 869 1059
Size: 869 190
SfcI C'TryA_G
Cuts at: 0 312 472 775 985 1059
Size: 312 160 303 210 74
Fragments arranged by size:
312 303 210 160 74
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 589 1059
Size: 589 470
SfoI GGC'GCC
Cuts at: 0 530 1059
Size: 530 529
SmaI CCC'GGG
Cuts at: 0 534 1059
Size: 534 525
SmlI C'TyrA_G
Cuts at: 0 318 646 1059
Size: 318 328 413
Fragments arranged by size:
413 328 318
SrfI GCCC'GGGC
Cuts at: 0 534 1059
Size: 534 525
StyI C'CwwG_G
Cuts at: 0 578 756 959 1046 1059
Size: 578 178 203 87 13
Fragments arranged by size:
578 203 178 87 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 135 1059
Size: 135 924
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 614 646 1059
Size: 614 32 413
Fragments arranged by size:
614 413 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 140 591 1059
Size: 140 451 468
Fragments arranged by size:
468 451 140
XhoI C'TCGA_G
Cuts at: 0 318 1059
Size: 318 741
XmaI C'CCGG_G
Cuts at: 0 532 1059
Size: 532 527
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI AlwNI ApoI
AvaI BaeI BanI BanII BbeI BbvCI BcgI BglI
BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI
BssHII BssSI BstAPI BstXI Bsu36I BtgI BtgZI DraIII
EaeI EagI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII KasI KpnI MscI MslI MspA1I NarI NcoI
PflMI PpuMI PspXI PstI PvuII RsrII SacII SanDI
SexAI SfcI SfiI SfoI SmaI SmlI SrfI StyI
TaqII TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BamHI BbsI
BciVI BclI BfrBI BglII BmrI BmtI BplI BsaI
BsaAI BsaBI BseRI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BstBI BstEII BstYI BstZ17I BtsI
ClaI CspCI DraI DrdI EarI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI MfeI MluI
MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PvuI SacI SalI SapI
SbfI ScaI SgfI SgrAI SnaBI SpeI SphI SspI
StuI SwaI TatI Tth111I XbaI XmnI ZraI