Restriction map for
pF1KE0787
(Linear) (Six-base) MAPSORT of: KIEE0787.seq Check: 7391 from: 1 to: 1059
KIEE0787.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 184 1059
Size: 184 875
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 1059
Size: 241 818
AleI CACnn'nnGTG
Cuts at: 0 97 742 1059
Size: 97 645 317
Fragments arranged by size:
645 317 97
AlwNI CAG_nnn'CTG
Cuts at: 0 339 639 1059
Size: 339 300 420
Fragments arranged by size:
420 339 300
AvaI C'yCGr_G
Cuts at: 0 263 1059
Size: 263 796
BamHI G'GATC_C
Cuts at: 0 149 819 1059
Size: 149 670 240
Fragments arranged by size:
670 240 149
BbsI GAAGACnn'nnnn_
Cuts at: 0 229 1059
Size: 229 830
BlpI GC'TnA_GC
Cuts at: 0 1019 1059
Size: 1019 40
BmrI ACTGGGnnnn_n'
Cuts at: 0 941 1059
Size: 941 118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 271 553 1059
Size: 208 63 282 506
Fragments arranged by size:
506 282 208 63
Bpu10I CC'TnA_GC
Cuts at: 0 715 1059
Size: 715 344
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 133 1059
Size: 1 132 926
Fragments arranged by size:
926 132 1
BsaWI w'CCGG_w
Cuts at: 0 421 1059
Size: 421 638
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 283 474 580 1059
Size: 199 84 191 106 479
Fragments arranged by size:
479 199 191 106 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 325 1059
Size: 150 175 734
Fragments arranged by size:
734 175 150
BsiEI CG_ry'CG
Cuts at: 0 205 1059
Size: 205 854
BsiHKAI G_wGCw'C
Cuts at: 0 17 721 1059
Size: 17 704 338
Fragments arranged by size:
704 338 17
Bsp1286I G_dGCh'C
Cuts at: 0 17 721 1059
Size: 17 704 338
Fragments arranged by size:
704 338 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 184 1059
Size: 184 875
BsrBI CCG'CTC
Cuts at: 0 207 273 1059
Size: 207 66 786
Fragments arranged by size:
786 207 66
BsrGI T'GTAC_A
Cuts at: 0 659 1059
Size: 659 400
BssSI C'ACGA_G
Cuts at: 0 898 1059
Size: 898 161
BstEII G'GTnAC_C
Cuts at: 0 540 1059
Size: 540 519
BstXI CCAn_nnnn'nTGG
Cuts at: 0 531 561 1059
Size: 531 30 498
Fragments arranged by size:
531 498 30
BstYI r'GATC_y
Cuts at: 0 149 621 819 1059
Size: 149 472 198 240
Fragments arranged by size:
472 240 198 149
Bsu36I CC'TnA_GG
Cuts at: 0 577 1059
Size: 577 482
BtgI C'CryG_G
Cuts at: 0 236 1059
Size: 236 823
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 84 373 1059
Size: 84 289 686
Fragments arranged by size:
686 289 84
EaeI y'GGCC_r
Cuts at: 0 521 626 761 1059
Size: 521 105 135 298
Fragments arranged by size:
521 298 135 105
EarI CTCTTCn'nnn_
Cuts at: 0 851 890 967 1059
Size: 851 39 77 92
Fragments arranged by size:
851 92 77 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 241 271 553 1059
Size: 208 33 30 282 506
Fragments arranged by size:
506 282 208 33 30
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 42 1059
Size: 42 1017
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 62 94 194 226 1059
Size: 62 32 100 32 833
Fragments arranged by size:
833 100 62 32 32
HindIII A'AGCT_T
Cuts at: 0 109 1059
Size: 109 950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 533 1059
Size: 103 430 526
Fragments arranged by size:
526 430 103
MscI TGG'CCA
Cuts at: 0 763 1059
Size: 763 296
MslI CAynn'nnrTG
Cuts at: 0 97 559 742 839 1059
Size: 97 462 183 97 220
Fragments arranged by size:
462 220 183 97 97
MspA1I CmG'CkG
Cuts at: 0 339 1059
Size: 339 720
PflMI CCAn_nnn'nTGG
Cuts at: 0 874 1059
Size: 874 185
PfoI T'CCnGG_A
Cuts at: 0 155 248 638 1059
Size: 155 93 390 421
Fragments arranged by size:
421 390 155 93
PvuII CAG'CTG
Cuts at: 0 339 1059
Size: 339 720
ScaI AGT'ACT
Cuts at: 0 244 1059
Size: 244 815
SmlI C'TyrA_G
Cuts at: 0 16 112 263 1059
Size: 16 96 151 796
Fragments arranged by size:
796 151 96 16
StuI AGG'CCT
Cuts at: 0 256 582 1059
Size: 256 326 477
Fragments arranged by size:
477 326 256
StyI C'CwwG_G
Cuts at: 0 140 698 1059
Size: 140 558 361
Fragments arranged by size:
558 361 140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 577 862 1059
Size: 577 285 197
Fragments arranged by size:
577 285 197
TatI w'GTAC_w
Cuts at: 0 101 242 659 1059
Size: 101 141 417 400
Fragments arranged by size:
417 400 141 101
XhoI C'TCGA_G
Cuts at: 0 263 1059
Size: 263 796
XmnI GAAnn'nnTTC
Cuts at: 0 429 1059
Size: 429 630
Enzymes that do cut:
AarI AcuI AleI AlwNI AvaI BamHI BbsI BlpI
BmrI BpmI Bpu10I BpuEI BsaWI BseRI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrGI BssSI BstEII BstXI
BstYI Bsu36I BtgI BtgZI EaeI EarI Eco57MI FalI
Hin4I HindIII MmeI MscI MslI MspA1I PflMI PfoI
PvuII ScaI SmlI StuI StyI TaqII TatI XhoI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmtI
BplI BsaI BsaAI BsaBI BsaHI BsaXI BseYI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrFI BssHII BstAPI
BstBI BstZ17I BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI
XcmI XmaI ZraI