(Linear) (Six-base) MAPSORT of: KIEE0791.seq  Check: 7593  from: 1  to: 1071



KIEE0791.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     303     405    1071 
   Size:        303     102     666

  Fragments arranged by size: 

                 666     303     102
 
AflIII A'CryG_T

Cuts at:      0     822    1071 
   Size:        822     249
 
AleI CACnn'nnGTG

Cuts at:      0     698    1071 
   Size:        698     373
 
ApoI r'AATT_y

Cuts at:      0     534     727    1071 
   Size:        534     193     344

  Fragments arranged by size: 

                 534     344     193
 
AseI AT'TA_AT

Cuts at:      0    1051    1071 
   Size:       1051      20
 
AvaI C'yCGr_G

Cuts at:      0     408    1071 
   Size:        408     663
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     611     644    1071 
   Size:        611      33     427

  Fragments arranged by size: 

                 611     427      33
 
BanII G_rGCy'C

Cuts at:      0     836    1071 
   Size:        836     235
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      55     265    1071 
   Size:         55     210     806

  Fragments arranged by size: 

                 806     210      55
 
BclI T'GATC_A

Cuts at:      0      84     923    1071 
   Size:         84     839     148

  Fragments arranged by size: 

                 839     148      84
 
BglII A'GATC_T

Cuts at:      0     314    1071 
   Size:        314     757
 
BmtI G_CTAG'C

Cuts at:      0     253     502    1071 
   Size:        253     249     569

  Fragments arranged by size: 

                 569     253     249
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     541     571    1071 
   Size:        541      30     500

  Fragments arranged by size: 

                 541     500      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     562    1071 
   Size:        562     509
 
BsiHKAI G_wGCw'C

Cuts at:      0     836    1071 
   Size:        836     235
 
BsmI GAATG_Cn'

Cuts at:      0     788    1071 
   Size:        788     283
 
Bsp1286I G_dGCh'C

Cuts at:      0     836    1071 
   Size:        836     235
 
BspHI T'CATG_A

Cuts at:      0    1043    1071 
   Size:       1043      28
 
BstYI r'GATC_y

Cuts at:      0     314     703    1006    1071 
   Size:        314     389     303      65

  Fragments arranged by size: 

                 389     314     303      65
 
BtgI C'CryG_G

Cuts at:      0     452    1071 
   Size:        452     619
 
DraI TTT'AAA

Cuts at:      0    1032    1071 
   Size:       1032      39
 
EaeI y'GGCC_r

Cuts at:      0      95    1071 
   Size:         95     976
 
EarI CTCTTCn'nnn_

Cuts at:      0     103    1071 
   Size:        103     968
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     155    1071 
   Size:        155     916
 
EcoICRI GAG'CTC

Cuts at:      0     834    1071 
   Size:        834     237
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     303     405    1071 
   Size:        303     102     666

  Fragments arranged by size: 

                 666     303     102
 
EcoO109I rG'GnC_Cy

Cuts at:      0     620    1071 
   Size:        620     451
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     132    1071 
   Size:        132     939
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     832     864    1071 
   Size:        832      32     207

  Fragments arranged by size: 

                 832     207      32
 
HincII GTy'rAC

Cuts at:      0      72    1071 
   Size:         72     999
 
HindIII A'AGCT_T

Cuts at:      0     417     868    1071 
   Size:        417     451     203

  Fragments arranged by size: 

                 451     417     203
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     190    1071 
   Size:        190     881
 
MslI CAynn'nnrTG

Cuts at:      0     305     698    1071 
   Size:        305     393     373

  Fragments arranged by size: 

                 393     373     305
 
NdeI CA'TA_TG

Cuts at:      0     189    1071 
   Size:        189     882
 
NheI G'CTAG_C

Cuts at:      0     249     498    1071 
   Size:        249     249     573

  Fragments arranged by size: 

                 573     249     249
 
NspI r_CATG'y

Cuts at:      0     271     512     826     856     938    1071 
   Size:        271     241     314      30      82     133

  Fragments arranged by size: 

                 314     271     241     133      82      30
 
PciI A'CATG_T

Cuts at:      0     822    1071 
   Size:        822     249
 
PpuMI rG'GwC_Cy

Cuts at:      0     620    1071 
   Size:        620     451
 
SacI G_AGCT'C

Cuts at:      0     836    1071 
   Size:        836     235
 
SapI GCTCTTCn'nnn_

Cuts at:      0     103    1071 
   Size:        103     968
 
SfcI C'TryA_G

Cuts at:      0     586    1071 
   Size:        586     485
 
SspI AAT'ATT

Cuts at:      0     197    1071 
   Size:        197     874
 
StyI C'CwwG_G

Cuts at:      0     176    1071 
   Size:        176     895
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     541     573    1071 
   Size:        541      32     498

  Fragments arranged by size: 

                 541     498      32
 
XbaI T'CTAG_A

Cuts at:      0     372    1071 
   Size:        372     699
 
XmnI GAAnn'nnTTC

Cuts at:      0     727    1071 
   Size:        727     344
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     ApoI     AseI     AvaI     BaeI    BanII
     BbsI     BclI    BglII     BmtI    BsaXI    BseRI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BstYI     BtgI     DraI     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I     FalI    Hin4I   HincII  HindIII     MmeI
     MslI     NdeI     NheI     NspI     PciI    PpuMI     SacI     SapI
     SfcI     SspI     StyI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
    AvrII    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EagI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII     HpaI
     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII
     TatI  Tth111I     XcmI     XhoI     XmaI     ZraI