Restriction map for
pF1KE0804
(Linear) (Six-base) MAPSORT of: KIEE0804.seq Check: 1444 from: 1 to: 867
KIEE0804.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 867
Size: 279 588
AfeI AGC'GCT
Cuts at: 0 307 867
Size: 307 560
AgeI A'CCGG_T
Cuts at: 0 78 867
Size: 78 789
AlwNI CAG_nnn'CTG
Cuts at: 0 666 849 867
Size: 666 183 18
Fragments arranged by size:
666 183 18
ApaI G_GGCC'C
Cuts at: 0 133 810 867
Size: 133 677 57
Fragments arranged by size:
677 133 57
AvaI C'yCGr_G
Cuts at: 0 70 750 769 867
Size: 70 680 19 98
Fragments arranged by size:
680 98 70 19
BanI G'GyrC_C
Cuts at: 0 223 820 832 867
Size: 223 597 12 35
Fragments arranged by size:
597 223 35 12
BanII G_rGCy'C
Cuts at: 0 133 278 810 867
Size: 133 145 532 57
Fragments arranged by size:
532 145 133 57
BbeI G_GCGC'C
Cuts at: 0 227 824 867
Size: 227 597 43
Fragments arranged by size:
597 227 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 36 636 867
Size: 36 600 231
Fragments arranged by size:
600 231 36
BbvCI CC'TCA_GC
Cuts at: 0 787 867
Size: 787 80
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 814 848 867
Size: 814 34 19
Fragments arranged by size:
814 34 19
BglI GCCn_nnn'nGGC
Cuts at: 0 194 526 867
Size: 194 332 341
Fragments arranged by size:
341 332 194
Bme1580I G_kGCm'C
Cuts at: 0 133 457 810 867
Size: 133 324 353 57
Fragments arranged by size:
353 324 133 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 672 867
Size: 42 630 195
Fragments arranged by size:
630 195 42
Bpu10I CC'TnA_GC
Cuts at: 0 209 787 867
Size: 209 578 80
Fragments arranged by size:
578 209 80
BsaI GGTCTCn'nnnn_
Cuts at: 0 865 867
Size: 865 2
BsaAI yAC'GTr
Cuts at: 0 480 867
Size: 480 387
BsaHI Gr'CG_yC
Cuts at: 0 224 821 867
Size: 224 597 46
Fragments arranged by size:
597 224 46
BsaWI w'CCGG_w
Cuts at: 0 78 867
Size: 78 789
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 715 745 867
Size: 715 30 122
Fragments arranged by size:
715 122 30
BseYI C'CCAG_C
Cuts at: 0 828 867
Size: 828 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 867
Size: 136 731
BsiEI CG_ry'CG
Cuts at: 0 368 407 867
Size: 368 39 460
Fragments arranged by size:
460 368 39
BsiHKAI G_wGCw'C
Cuts at: 0 101 251 867
Size: 101 150 616
Fragments arranged by size:
616 150 101
Bsp1286I G_dGCh'C
Cuts at: 0 101 133 251 278 457 810 867
Size: 101 32 118 27 179 353 57
Fragments arranged by size:
353 179 118 101 57 32 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 155 565 867
Size: 155 410 302
Fragments arranged by size:
410 302 155
BsrFI r'CCGG_y
Cuts at: 0 78 347 402 867
Size: 78 269 55 465
Fragments arranged by size:
465 269 78 55
BssHII G'CGCG_C
Cuts at: 0 605 867
Size: 605 262
BstAPI GCAn_nnn'nTGC
Cuts at: 0 666 867
Size: 666 201
BstEII G'GTnAC_C
Cuts at: 0 552 867
Size: 552 315
BstXI CCAn_nnnn'nTGG
Cuts at: 0 711 867
Size: 711 156
BtgI C'CryG_G
Cuts at: 0 203 574 854 867
Size: 203 371 280 13
Fragments arranged by size:
371 280 203 13
DrdI GACnn_nn'nnGTC
Cuts at: 0 40 867
Size: 40 827
EaeI y'GGCC_r
Cuts at: 0 158 365 404 482 701 867
Size: 158 207 39 78 219 166
Fragments arranged by size:
219 207 166 158 78 39
EagI C'GGCC_G
Cuts at: 0 365 404 867
Size: 365 39 463
Fragments arranged by size:
463 365 39
EarI CTCTTCn'nnn_
Cuts at: 0 328 625 867
Size: 328 297 242
Fragments arranged by size:
328 297 242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 279 672 867
Size: 42 237 393 195
Fragments arranged by size:
393 237 195 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 814 867
Size: 814 53
EcoO109I rG'GnC_Cy
Cuts at: 0 129 130 806 807 867
Size: 129 1 676 1 60
Fragments arranged by size:
676 129 60 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 150 867
Size: 150 717
FspI TGC'GCA
Cuts at: 0 442 867
Size: 442 425
HaeII r_GCGC'y
Cuts at: 0 227 309 824 867
Size: 227 82 515 43
Fragments arranged by size:
515 227 82 43
HincII GTy'rAC
Cuts at: 0 723 867
Size: 723 144
KasI G'GCGC_C
Cuts at: 0 223 820 867
Size: 223 597 47
Fragments arranged by size:
597 223 47
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 867
Size: 341 526
MscI TGG'CCA
Cuts at: 0 484 703 867
Size: 484 219 164
Fragments arranged by size:
484 219 164
MspA1I CmG'CkG
Cuts at: 0 414 576 699 786 867
Size: 414 162 123 87 81
Fragments arranged by size:
414 162 123 87 81
NaeI GCC'GGC
Cuts at: 0 349 404 867
Size: 349 55 463
Fragments arranged by size:
463 349 55
NarI GG'CG_CC
Cuts at: 0 224 821 867
Size: 224 597 46
Fragments arranged by size:
597 224 46
NcoI C'CATG_G
Cuts at: 0 854 867
Size: 854 13
NgoMIV G'CCGG_C
Cuts at: 0 347 402 867
Size: 347 55 465
Fragments arranged by size:
465 347 55
PasI CC'CwG_GG
Cuts at: 0 219 810 867
Size: 219 591 57
Fragments arranged by size:
591 219 57
PmlI CAC'GTG
Cuts at: 0 480 867
Size: 480 387
PspOMI G'GGCC_C
Cuts at: 0 129 806 867
Size: 129 677 61
Fragments arranged by size:
677 129 61
PstI C_TGCA'G
Cuts at: 0 662 867
Size: 662 205
SacII CC_GC'GG
Cuts at: 0 577 867
Size: 577 290
SapI GCTCTTCn'nnn_
Cuts at: 0 625 867
Size: 625 242
SexAI A'CCwGG_T
Cuts at: 0 493 867
Size: 493 374
SfcI C'TryA_G
Cuts at: 0 658 867
Size: 658 209
SfoI GGC'GCC
Cuts at: 0 225 822 867
Size: 225 597 45
Fragments arranged by size:
597 225 45
SmaI CCC'GGG
Cuts at: 0 771 867
Size: 771 96
StyI C'CwwG_G
Cuts at: 0 854 867
Size: 854 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 540 630 820 867
Size: 540 90 190 47
Fragments arranged by size:
540 190 90 47
XmaI C'CCGG_G
Cuts at: 0 769 867
Size: 769 98
Enzymes that do cut:
AcuI AfeI AgeI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BglI Bme1580I BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrFI BssHII BstAPI BstEII BstXI
BtgI DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I
FalI FspI HaeII HincII KasI MmeI MscI MspA1I
NaeI NarI NcoI NgoMIV PasI PmlI PspOMI PstI
SacII SapI SexAI SfcI SfoI SmaI StyI TaqII
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI BpuEI BsaBI BseRI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssSI
BstBI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII EciI EcoICRI EcoRI EcoRV FseI FspAI
Hin4I HindIII HpaI KpnI MfeI MluI MslI NdeI
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmnI ZraI