Restriction map for
pF1KE0805
(Linear) (Six-base) MAPSORT of: KIEE0805.seq Check: 7291 from: 1 to: 1080
KIEE0805.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 795 919 1080
Size: 795 124 161
Fragments arranged by size:
795 161 124
AccI GT'mk_AC
Cuts at: 0 530 653 1080
Size: 530 123 427
Fragments arranged by size:
530 427 123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 1080
Size: 171 909
AflIII A'CryG_T
Cuts at: 0 737 1080
Size: 737 343
ApaI G_GGCC'C
Cuts at: 0 879 1080
Size: 879 201
ApaLI G'TGCA_C
Cuts at: 0 733 1080
Size: 733 347
AvaI C'yCGr_G
Cuts at: 0 235 1080
Size: 235 845
BanI G'GyrC_C
Cuts at: 0 721 1080
Size: 721 359
BanII G_rGCy'C
Cuts at: 0 83 879 1080
Size: 83 796 201
Fragments arranged by size:
796 201 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 216 1080
Size: 216 864
BfrBI ATG'CAT
Cuts at: 0 821 1080
Size: 821 259
Bme1580I G_kGCm'C
Cuts at: 0 724 737 879 1080
Size: 724 13 142 201
Fragments arranged by size:
724 201 142 13
BmgBI CAC'GTC
Cuts at: 0 762 1080
Size: 762 318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 97 409 1080
Size: 43 54 312 671
Fragments arranged by size:
671 312 54 43
BsaI GGTCTCn'nnnn_
Cuts at: 0 385 1080
Size: 385 695
BsaAI yAC'GTr
Cuts at: 0 732 1080
Size: 732 348
BsaBI GATnn'nnATC
Cuts at: 0 406 484 1080
Size: 406 78 596
Fragments arranged by size:
596 406 78
BsaHI Gr'CG_yC
Cuts at: 0 629 1080
Size: 629 451
BsaWI w'CCGG_w
Cuts at: 0 1013 1080
Size: 1013 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 20 50 304 334 1080
Size: 20 30 254 30 746
Fragments arranged by size:
746 254 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 703 1080
Size: 703 377
BseYI C'CCAG_C
Cuts at: 0 83 872 1080
Size: 83 789 208
Fragments arranged by size:
789 208 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 742 1080
Size: 301 441 338
Fragments arranged by size:
441 338 301
BsiHKAI G_wGCw'C
Cuts at: 0 170 737 1080
Size: 170 567 343
Fragments arranged by size:
567 343 170
BsmI GAATG_Cn'
Cuts at: 0 120 1080
Size: 120 960
Bsp1286I G_dGCh'C
Cuts at: 0 83 170 724 737 879 1080
Size: 83 87 554 13 142 201
Fragments arranged by size:
554 201 142 87 83 13
BspEI T'CCGG_A
Cuts at: 0 1013 1080
Size: 1013 67
BspHI T'CATG_A
Cuts at: 0 398 575 1080
Size: 398 177 505
Fragments arranged by size:
505 398 177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 210 323 795 919 1080
Size: 210 113 472 124 161
Fragments arranged by size:
472 210 161 124 113
BsrDI GCAATG_nn'
Cuts at: 0 116 1080
Size: 116 964
BstAPI GCAn_nnn'nTGC
Cuts at: 0 115 1080
Size: 115 965
BstXI CCAn_nnnn'nTGG
Cuts at: 0 387 852 1080
Size: 387 465 228
Fragments arranged by size:
465 387 228
BtgI C'CryG_G
Cuts at: 0 296 1080
Size: 296 784
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 507 1080
Size: 507 573
BtsI GCAGTG_nn'
Cuts at: 0 148 778 941 1080
Size: 148 630 163 139
Fragments arranged by size:
630 163 148 139
DrdI GACnn_nn'nnGTC
Cuts at: 0 538 1080
Size: 538 542
EaeI y'GGCC_r
Cuts at: 0 293 356 518 608 1080
Size: 293 63 162 90 472
Fragments arranged by size:
472 293 162 90 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 97 171 409 1080
Size: 43 54 74 238 671
Fragments arranged by size:
671 238 74 54 43
EcoO109I rG'GnC_Cy
Cuts at: 0 591 876 1080
Size: 591 285 204
Fragments arranged by size:
591 285 204
FspI TGC'GCA
Cuts at: 0 1030 1080
Size: 1030 50
HaeII r_GCGC'y
Cuts at: 0 209 1080
Size: 209 871
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 20 52 1080
Size: 20 32 1028
Fragments arranged by size:
1028 32 20
HincII GTy'rAC
Cuts at: 0 531 1080
Size: 531 549
MscI TGG'CCA
Cuts at: 0 520 610 1080
Size: 520 90 470
Fragments arranged by size:
520 470 90
MslI CAynn'nnrTG
Cuts at: 0 580 691 1080
Size: 580 111 389
Fragments arranged by size:
580 389 111
NsiI A_TGCA'T
Cuts at: 0 823 1080
Size: 823 257
NspI r_CATG'y
Cuts at: 0 366 741 1080
Size: 366 375 339
Fragments arranged by size:
375 366 339
PciI A'CATG_T
Cuts at: 0 737 1080
Size: 737 343
PflMI CCAn_nnn'nTGG
Cuts at: 0 456 1080
Size: 456 624
PspOMI G'GGCC_C
Cuts at: 0 875 1080
Size: 875 205
PstI C_TGCA'G
Cuts at: 0 346 1080
Size: 346 734
SalI G'TCGA_C
Cuts at: 0 529 1080
Size: 529 551
SexAI A'CCwGG_T
Cuts at: 0 893 962 1080
Size: 893 69 118
Fragments arranged by size:
893 118 69
SfcI C'TryA_G
Cuts at: 0 342 1080
Size: 342 738
TatI w'GTAC_w
Cuts at: 0 329 1080
Size: 329 751
Enzymes that do cut:
AarI AccI AcuI AflIII ApaI ApaLI AvaI BanI
BanII BbsI BfrBI Bme1580I BmgBI BpmI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrDI BstAPI BstXI
BtgI BtgZI BtsI DrdI EaeI Eco57MI EcoO109I FspI
HaeII Hin4I HincII MscI MslI NsiI NspI PciI
PflMI PspOMI PstI SalI SexAI SfcI TatI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI BmrI BmtI BplI Bpu10I BpuEI BsiEI
BsiWI BsmBI BsrBI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII
EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspAI HindIII HpaI KasI KpnI MfeI MluI
MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PasI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SacII SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI