Restriction map for
pF1KE0812
(Linear) (Six-base) MAPSORT of: KIEE0812.seq Check: 8965 from: 1 to: 936
KIEE0812.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 727 936
Size: 727 209
AccI GT'mk_AC
Cuts at: 0 773 936
Size: 773 163
AclI AA'CG_TT
Cuts at: 0 605 936
Size: 605 331
AflII C'TTAA_G
Cuts at: 0 675 936
Size: 675 261
ApaI G_GGCC'C
Cuts at: 0 761 936
Size: 761 175
ApoI r'AATT_y
Cuts at: 0 818 936
Size: 818 118
AvaI C'yCGr_G
Cuts at: 0 319 936
Size: 319 617
BanII G_rGCy'C
Cuts at: 0 761 936
Size: 761 175
BbsI GAAGACnn'nnnn_
Cuts at: 0 143 936
Size: 143 793
BlpI GC'TnA_GC
Cuts at: 0 262 936
Size: 262 674
Bme1580I G_kGCm'C
Cuts at: 0 442 761 936
Size: 442 319 175
Fragments arranged by size:
442 319 175
BmrI ACTGGGnnnn_n'
Cuts at: 0 127 936
Size: 127 809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 936
Size: 88 848
Bpu10I CC'TnA_GC
Cuts at: 0 579 936
Size: 579 357
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 888 936
Size: 888 48
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 210 936
Size: 210 726
BseYI C'CCAG_C
Cuts at: 0 801 936
Size: 801 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 936
Size: 93 843
BsiHKAI G_wGCw'C
Cuts at: 0 729 936
Size: 729 207
BsmI GAATG_Cn'
Cuts at: 0 458 680 936
Size: 458 222 256
Fragments arranged by size:
458 256 222
Bsp1286I G_dGCh'C
Cuts at: 0 442 729 761 936
Size: 442 287 32 175
Fragments arranged by size:
442 287 175 32
BspHI T'CATG_A
Cuts at: 0 404 824 936
Size: 404 420 112
Fragments arranged by size:
420 404 112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 727 785 936
Size: 727 58 151
Fragments arranged by size:
727 151 58
BsrDI GCAATG_nn'
Cuts at: 0 734 936
Size: 734 202
BstAPI GCAn_nnn'nTGC
Cuts at: 0 332 936
Size: 332 604
BstXI CCAn_nnnn'nTGG
Cuts at: 0 519 936
Size: 519 417
BstYI r'GATC_y
Cuts at: 0 416 936
Size: 416 520
BtgI C'CryG_G
Cuts at: 0 809 936
Size: 809 127
BtsI GCAGTG_nn'
Cuts at: 0 368 729 936
Size: 368 361 207
Fragments arranged by size:
368 361 207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 936
Size: 88 848
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 383 415 936
Size: 383 32 521
Fragments arranged by size:
521 383 32
MslI CAynn'nnrTG
Cuts at: 0 172 936
Size: 172 764
PflMI CCAn_nnn'nTGG
Cuts at: 0 505 936
Size: 505 431
PspOMI G'GGCC_C
Cuts at: 0 757 936
Size: 757 179
PstI C_TGCA'G
Cuts at: 0 724 782 936
Size: 724 58 154
Fragments arranged by size:
724 154 58
SfcI C'TryA_G
Cuts at: 0 720 778 936
Size: 720 58 158
Fragments arranged by size:
720 158 58
SmlI C'TyrA_G
Cuts at: 0 675 867 936
Size: 675 192 69
Fragments arranged by size:
675 192 69
SspI AAT'ATT
Cuts at: 0 905 936
Size: 905 31
StyI C'CwwG_G
Cuts at: 0 412 557 936
Size: 412 145 379
Fragments arranged by size:
412 379 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 804 936
Size: 804 132
Tth111I GACn'n_nGTC
Cuts at: 0 289 936
Size: 289 647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 798 936
Size: 798 138
Enzymes that do cut:
AarI AccI AclI AflII ApaI ApoI AvaI BanII
BbsI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BstAPI BstXI BstYI BtgI BtsI Eco57MI Hin4I MslI
PflMI PspOMI PstI SfcI SmlI SspI StyI TaqII
Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AcuI AfeI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BmgBI BmtI BplI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TatI TstI XbaI XhoI XmaI
XmnI ZraI