Restriction map for
pF1KE0822
(Linear) (Six-base) MAPSORT of: KIEE0822.seq Check: 3652 from: 1 to: 696
KIEE0822.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AatII G_ACGT'C
Cuts at: 0 546 696
Size: 546 150
AflII C'TTAA_G
Cuts at: 0 240 696
Size: 240 456
AflIII A'CryG_T
Cuts at: 0 332 413 478 696
Size: 332 81 65 218
Fragments arranged by size:
332 218 81 65
AleI CACnn'nnGTG
Cuts at: 0 598 696
Size: 598 98
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 111 696
Size: 111 585
ApoI r'AATT_y
Cuts at: 0 9 696
Size: 9 687
BbsI GAAGACnn'nnnn_
Cuts at: 0 34 696
Size: 34 662
BclI T'GATC_A
Cuts at: 0 395 696
Size: 395 301
BfrBI ATG'CAT
Cuts at: 0 534 696
Size: 534 162
BsaAI yAC'GTr
Cuts at: 0 335 696
Size: 335 361
BsaHI Gr'CG_yC
Cuts at: 0 543 696
Size: 543 153
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 241 271 696
Size: 241 30 425
Fragments arranged by size:
425 241 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 296 696
Size: 296 400
BsiHKAI G_wGCw'C
Cuts at: 0 95 696
Size: 95 601
BsmBI CGTCTCn'nnnn_
Cuts at: 0 534 696
Size: 534 162
Bsp1286I G_dGCh'C
Cuts at: 0 95 696
Size: 95 601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 34 696
Size: 34 662
BsrDI GCAATG_nn'
Cuts at: 0 71 145 696
Size: 71 74 551
Fragments arranged by size:
551 74 71
BsrFI r'CCGG_y
Cuts at: 0 367 696
Size: 367 329
BsrGI T'GTAC_A
Cuts at: 0 356 696
Size: 356 340
Bsu36I CC'TnA_GG
Cuts at: 0 634 696
Size: 634 62
BtgI C'CryG_G
Cuts at: 0 560 696
Size: 560 136
BtsI GCAGTG_nn'
Cuts at: 0 64 93 696
Size: 64 29 603
Fragments arranged by size:
603 64 29
EaeI y'GGCC_r
Cuts at: 0 131 696
Size: 131 565
EarI CTCTTCn'nnn_
Cuts at: 0 395 529 696
Size: 395 134 167
Fragments arranged by size:
395 167 134
EcoO109I rG'GnC_Cy
Cuts at: 0 245 696
Size: 245 451
HindIII A'AGCT_T
Cuts at: 0 467 696
Size: 467 229
MscI TGG'CCA
Cuts at: 0 133 696
Size: 133 563
MslI CAynn'nnrTG
Cuts at: 0 76 598 696
Size: 76 522 98
Fragments arranged by size:
522 98 76
NaeI GCC'GGC
Cuts at: 0 369 696
Size: 369 327
NdeI CA'TA_TG
Cuts at: 0 117 696
Size: 117 579
NgoMIV G'CCGG_C
Cuts at: 0 367 696
Size: 367 329
NsiI A_TGCA'T
Cuts at: 0 536 696
Size: 536 160
NspI r_CATG'y
Cuts at: 0 102 326 417 482 534 696
Size: 102 224 91 65 52 162
Fragments arranged by size:
224 162 102 91 65 52
PciI A'CATG_T
Cuts at: 0 413 478 696
Size: 413 65 218
Fragments arranged by size:
413 218 65
PpuMI rG'GwC_Cy
Cuts at: 0 245 696
Size: 245 451
PshAI GACnn'nnGTC
Cuts at: 0 499 696
Size: 499 197
PstI C_TGCA'G
Cuts at: 0 453 696
Size: 453 243
SexAI A'CCwGG_T
Cuts at: 0 188 696
Size: 188 508
SfcI C'TryA_G
Cuts at: 0 153 372 449 696
Size: 153 219 77 247
Fragments arranged by size:
247 219 153 77
SmlI C'TyrA_G
Cuts at: 0 240 696
Size: 240 456
SphI G_CATG'C
Cuts at: 0 326 534 696
Size: 326 208 162
Fragments arranged by size:
326 208 162
StyI C'CwwG_G
Cuts at: 0 171 696
Size: 171 525
TatI w'GTAC_w
Cuts at: 0 356 696
Size: 356 340
XmnI GAAnn'nnTTC
Cuts at: 0 444 696
Size: 444 252
ZraI GAC'GTC
Cuts at: 0 544 696
Size: 544 152
Enzymes that do cut:
AatII AflII AflIII AleI AlfI ApoI BbsI BclI
BfrBI BsaAI BsaHI BsaXI BseRI BsiHKAI BsmBI Bsp1286I
BspMI BsrDI BsrFI BsrGI Bsu36I BtgI BtsI EaeI
EarI EcoO109I HindIII MscI MslI NaeI NdeI NgoMIV
NsiI NspI PciI PpuMI PshAI PstI SexAI SfcI
SmlI SphI StyI TatI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BseYI
BsgI BsiEI BsiWI BsmI BspEI BspHI BsrBI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MmeI MspA1I NarI NcoI NheI NotI NruI PacI
PasI PflMI PfoI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI