(Linear) (Six-base) MAPSORT of: KIEE0827.seq  Check: 120  from: 1  to: 660



KIEE0827.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AccI GT'mk_AC

Cuts at:      0     421     660 
   Size:        421     239
 
AflIII A'CryG_T

Cuts at:      0     248     569     660 
   Size:        248     321      91

  Fragments arranged by size: 

                 321     248      91
 
ApaLI G'TGCA_C

Cuts at:      0     273     660 
   Size:        273     387
 
ApoI r'AATT_y

Cuts at:      0     514     579     660 
   Size:        514      65      81

  Fragments arranged by size: 

                 514      81      65
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     196     660 
   Size:        196     464
 
BglII A'GATC_T

Cuts at:      0      41     660 
   Size:         41     619
 
Bme1580I G_kGCm'C

Cuts at:      0     277     660 
   Size:        277     383
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     179     660 
   Size:        179     481
 
BsaAI yAC'GTr

Cuts at:      0      78     660 
   Size:         78     582
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     643     660 
   Size:        643      17
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     660 
   Size:         82     578
 
BsiHKAI G_wGCw'C

Cuts at:      0     277     660 
   Size:        277     383
 
Bsp1286I G_dGCh'C

Cuts at:      0     277     660 
   Size:        277     383
 
BspHI T'CATG_A

Cuts at:      0     113     660 
   Size:        113     547
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     433     660 
   Size:        433     227
 
BsrDI GCAATG_nn'

Cuts at:      0     329     660 
   Size:        329     331
 
BsrGI T'GTAC_A

Cuts at:      0     197     660 
   Size:        197     463
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      28     660 
   Size:         28     632
 
BstYI r'GATC_y

Cuts at:      0      41     660 
   Size:         41     619
 
BtgI C'CryG_G

Cuts at:      0     443     660 
   Size:        443     217
 
BtsI GCAGTG_nn'

Cuts at:      0      94     660 
   Size:         94     566
 
EaeI y'GGCC_r

Cuts at:      0      80     149     660 
   Size:         80      69     511

  Fragments arranged by size: 

                 511      80      69
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     179     660 
   Size:        179     481
 
MscI TGG'CCA

Cuts at:      0      82     151     660 
   Size:         82      69     509

  Fragments arranged by size: 

                 509      82      69
 
MslI CAynn'nnrTG

Cuts at:      0     442     547     660 
   Size:        442     105     113

  Fragments arranged by size: 

                 442     113     105
 
NspI r_CATG'y

Cuts at:      0     252     660 
   Size:        252     408
 
PciI A'CATG_T

Cuts at:      0     248     660 
   Size:        248     412
 
PstI C_TGCA'G

Cuts at:      0     430     660 
   Size:        430     230
 
SfcI C'TryA_G

Cuts at:      0     302     426     629     660 
   Size:        302     124     203      31

  Fragments arranged by size: 

                 302     203     124      31
 
StuI AGG'CCT

Cuts at:      0     296     660 
   Size:        296     364
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     561     660 
   Size:        561      99
 
TatI w'GTAC_w

Cuts at:      0     197     660 
   Size:        197     463
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     256     288     660 
   Size:        256      32     372

  Fragments arranged by size: 

                 372     256      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      28     138     660 
   Size:         28     110     522

  Fragments arranged by size: 

                 522     110      28
 

 Enzymes that do cut:

     AccI   AflIII    ApaLI     ApoI     BbsI    BglII Bme1580I     BpmI
    BsaAI    BseRI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI
    BsrGI    BstXI    BstYI     BtgI     BtsI     EaeI  Eco57MI     MscI
     MslI     NspI     PciI     PstI     SfcI     StuI    TaqII     TatI
     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StyI     SwaI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI