(Linear) (Six-base) MAPSORT of: KIEE0830.seq  Check: 4495  from: 1  to: 759



KIEE0830.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     221     759 
   Size:        221     538
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     759 
   Size:        400     359
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     229     759 
   Size:        229     530
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     667     759 
   Size:        667      92
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     380     759 
   Size:        380     379
 
BglII A'GATC_T

Cuts at:      0      33     158     759 
   Size:         33     125     601

  Fragments arranged by size: 

                 601     125      33
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     284     759 
   Size:        284     475
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     759 
   Size:        199     560
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     221     759 
   Size:        221     538
 
BsrDI GCAATG_nn'

Cuts at:      0     182     656     759 
   Size:        182     474     103

  Fragments arranged by size: 

                 474     182     103
 
BstYI r'GATC_y

Cuts at:      0      33     158     759 
   Size:         33     125     601

  Fragments arranged by size: 

                 601     125      33
 
EarI CTCTTCn'nnn_

Cuts at:      0     595     649     759 
   Size:        595      54     110

  Fragments arranged by size: 

                 595     110      54
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     759 
   Size:        400     359
 
MfeI C'AATT_G

Cuts at:      0     739     759 
   Size:        739      20
 
NspI r_CATG'y

Cuts at:      0     690     759 
   Size:        690      69
 
ScaI AGT'ACT

Cuts at:      0     355     759 
   Size:        355     404
 
SfcI C'TryA_G

Cuts at:      0     543     759 
   Size:        543     216
 
StuI AGG'CCT

Cuts at:      0     437     759 
   Size:        437     322
 
StyI C'CwwG_G

Cuts at:      0     144     759 
   Size:        144     615
 
TatI w'GTAC_w

Cuts at:      0     271     353     759 
   Size:        271      82     406

  Fragments arranged by size: 

                 406     271      82
 

 Enzymes that do cut:

     AarI     AcuI     AlfI     BbsI    BciVI    BglII     BmrI     BsgI
    BspMI    BsrDI    BstYI     EarI  Eco57MI     MfeI     NspI     ScaI
     SfcI     StuI     StyI     TatI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI