(Linear) (Six-base) MAPSORT of: KIEE0833.seq Check: 3376 from: 1 to: 672 KIEE0833.orf With 182 enzymes: SgfI * April 24, 2009 13:18 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 568 672 Size: 568 104 ApoI r'AATT_y Cuts at: 0 6 25 672 Size: 6 19 647 Fragments arranged by size: 647 19 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 651 672 Size: 651 21 BbvCI CC'TCA_GC Cuts at: 0 570 616 672 Size: 570 46 56 Fragments arranged by size: 570 56 46 BmrI ACTGGGnnnn_n' Cuts at: 0 532 672 Size: 532 140 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 598 672 Size: 82 516 74 Fragments arranged by size: 516 82 74 Bpu10I CC'TnA_GC Cuts at: 0 570 616 672 Size: 570 46 56 Fragments arranged by size: 570 56 46 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 135 672 Size: 135 537 BseYI C'CCAG_C Cuts at: 0 36 290 672 Size: 36 254 382 Fragments arranged by size: 382 254 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 303 581 672 Size: 303 278 91 Fragments arranged by size: 303 278 91 BsiEI CG_ry'CG Cuts at: 0 357 672 Size: 357 315 BsmI GAATG_Cn' Cuts at: 0 452 672 Size: 452 220 BspHI T'CATG_A Cuts at: 0 398 672 Size: 398 274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 128 672 Size: 128 544 BsrGI T'GTAC_A Cuts at: 0 335 672 Size: 335 337 BstAPI GCAn_nnn'nTGC Cuts at: 0 283 672 Size: 283 389 BstXI CCAn_nnnn'nTGG Cuts at: 0 513 672 Size: 513 159 BtgI C'CryG_G Cuts at: 0 428 672 Size: 428 244 DraIII CAC_nnn'GTG Cuts at: 0 326 672 Size: 326 346 EarI CTCTTCn'nnn_ Cuts at: 0 301 672 Size: 301 371 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 568 598 672 Size: 82 486 30 74 Fragments arranged by size: 486 82 74 30 EcoO109I rG'GnC_Cy Cuts at: 0 434 672 Size: 434 238 EcoRI G'AATT_C Cuts at: 0 25 672 Size: 25 647 EcoRV GAT'ATC Cuts at: 0 534 672 Size: 534 138 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 401 433 672 Size: 401 32 239 Fragments arranged by size: 401 239 32 MfeI C'AATT_G Cuts at: 0 487 672 Size: 487 185 MslI CAynn'nnrTG Cuts at: 0 109 166 343 397 598 672 Size: 109 57 177 54 201 74 Fragments arranged by size: 201 177 109 74 57 54 MspA1I CmG'CkG Cuts at: 0 620 672 Size: 620 52 NcoI C'CATG_G Cuts at: 0 428 672 Size: 428 244 PflMI CCAn_nnn'nTGG Cuts at: 0 312 672 Size: 312 360 PpuMI rG'GwC_Cy Cuts at: 0 434 672 Size: 434 238 PvuII CAG'CTG Cuts at: 0 620 672 Size: 620 52 SapI GCTCTTCn'nnn_ Cuts at: 0 301 672 Size: 301 371 SfcI C'TryA_G Cuts at: 0 645 672 Size: 645 27 StyI C'CwwG_G Cuts at: 0 428 672 Size: 428 244 TatI w'GTAC_w Cuts at: 0 170 335 672 Size: 170 165 337 Fragments arranged by size: 337 170 165 Enzymes that do cut: AcuI ApoI BbsI BbvCI BmrI BpmI Bpu10I BseRI BseYI BsgI BsiEI BsmI BspHI BspMI BsrGI BstAPI BstXI BtgI DraIII EarI Eco57MI EcoO109I EcoRI EcoRV Hin4I MfeI MslI MspA1I NcoI PflMI PpuMI PvuII SapI SfcI StyI TatI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoNI FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI