(Linear) (Six-base) MAPSORT of: KIEE0833.seq  Check: 3376  from: 1  to: 672



KIEE0833.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     568     672 
   Size:        568     104
 
ApoI r'AATT_y

Cuts at:      0       6      25     672 
   Size:          6      19     647

  Fragments arranged by size: 

                 647      19       6
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     651     672 
   Size:        651      21
 
BbvCI CC'TCA_GC

Cuts at:      0     570     616     672 
   Size:        570      46      56

  Fragments arranged by size: 

                 570      56      46
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     532     672 
   Size:        532     140
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     598     672 
   Size:         82     516      74

  Fragments arranged by size: 

                 516      82      74
 
Bpu10I CC'TnA_GC

Cuts at:      0     570     616     672 
   Size:        570      46      56

  Fragments arranged by size: 

                 570      56      46
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     135     672 
   Size:        135     537
 
BseYI C'CCAG_C

Cuts at:      0      36     290     672 
   Size:         36     254     382

  Fragments arranged by size: 

                 382     254      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     303     581     672 
   Size:        303     278      91

  Fragments arranged by size: 

                 303     278      91
 
BsiEI CG_ry'CG

Cuts at:      0     357     672 
   Size:        357     315
 
BsmI GAATG_Cn'

Cuts at:      0     452     672 
   Size:        452     220
 
BspHI T'CATG_A

Cuts at:      0     398     672 
   Size:        398     274
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     128     672 
   Size:        128     544
 
BsrGI T'GTAC_A

Cuts at:      0     335     672 
   Size:        335     337
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     283     672 
   Size:        283     389
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     513     672 
   Size:        513     159
 
BtgI C'CryG_G

Cuts at:      0     428     672 
   Size:        428     244
 
DraIII CAC_nnn'GTG

Cuts at:      0     326     672 
   Size:        326     346
 
EarI CTCTTCn'nnn_

Cuts at:      0     301     672 
   Size:        301     371
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     568     598     672 
   Size:         82     486      30      74

  Fragments arranged by size: 

                 486      82      74      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0     434     672 
   Size:        434     238
 
EcoRI G'AATT_C

Cuts at:      0      25     672 
   Size:         25     647
 
EcoRV GAT'ATC

Cuts at:      0     534     672 
   Size:        534     138
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     401     433     672 
   Size:        401      32     239

  Fragments arranged by size: 

                 401     239      32
 
MfeI C'AATT_G

Cuts at:      0     487     672 
   Size:        487     185
 
MslI CAynn'nnrTG

Cuts at:      0     109     166     343     397     598     672 
   Size:        109      57     177      54     201      74

  Fragments arranged by size: 

                 201     177     109      74      57      54
 
MspA1I CmG'CkG

Cuts at:      0     620     672 
   Size:        620      52
 
NcoI C'CATG_G

Cuts at:      0     428     672 
   Size:        428     244
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     312     672 
   Size:        312     360
 
PpuMI rG'GwC_Cy

Cuts at:      0     434     672 
   Size:        434     238
 
PvuII CAG'CTG

Cuts at:      0     620     672 
   Size:        620      52
 
SapI GCTCTTCn'nnn_

Cuts at:      0     301     672 
   Size:        301     371
 
SfcI C'TryA_G

Cuts at:      0     645     672 
   Size:        645      27
 
StyI C'CwwG_G

Cuts at:      0     428     672 
   Size:        428     244
 
TatI w'GTAC_w

Cuts at:      0     170     335     672 
   Size:        170     165     337

  Fragments arranged by size: 

                 337     170     165
 

 Enzymes that do cut:

     AcuI     ApoI     BbsI    BbvCI     BmrI     BpmI   Bpu10I    BseRI
    BseYI     BsgI    BsiEI     BsmI    BspHI    BspMI    BsrGI   BstAPI
    BstXI     BtgI   DraIII     EarI  Eco57MI EcoO109I    EcoRI    EcoRV
    Hin4I     MfeI     MslI   MspA1I     NcoI    PflMI    PpuMI    PvuII
     SapI     SfcI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI