Restriction map for
pF1KE0836
(Linear) (Six-base) MAPSORT of: KIEE0836.seq Check: 5421 from: 1 to: 954
KIEE0836.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 876 940 954
Size: 420 456 64 14
Fragments arranged by size:
456 420 64 14
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 658 954
Size: 658 296
AlwNI CAG_nnn'CTG
Cuts at: 0 899 954
Size: 899 55
ApoI r'AATT_y
Cuts at: 0 571 829 954
Size: 571 258 125
Fragments arranged by size:
571 258 125
AvrII C'CTAG_G
Cuts at: 0 183 954
Size: 183 771
BanII G_rGCy'C
Cuts at: 0 452 710 954
Size: 452 258 244
Fragments arranged by size:
452 258 244
BbsI GAAGACnn'nnnn_
Cuts at: 0 339 378 669 954
Size: 339 39 291 285
Fragments arranged by size:
339 291 285 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 704 954
Size: 704 250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 954
Size: 438 516
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 921 954
Size: 921 33
BsaI GGTCTCn'nnnn_
Cuts at: 0 2 954
Size: 2 952
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 549 579 954
Size: 549 30 375
Fragments arranged by size:
549 375 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 736 954
Size: 736 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 884 954
Size: 736 148 70
Fragments arranged by size:
736 148 70
BsmI GAATG_Cn'
Cuts at: 0 315 954
Size: 315 639
Bsp1286I G_dGCh'C
Cuts at: 0 452 710 954
Size: 452 258 244
Fragments arranged by size:
452 258 244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 793 954
Size: 793 161
BsrDI GCAATG_nn'
Cuts at: 0 108 954
Size: 108 846
BstEII G'GTnAC_C
Cuts at: 0 60 954
Size: 60 894
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 954
Size: 57 897
BstYI r'GATC_y
Cuts at: 0 25 954
Size: 25 929
Bsu36I CC'TnA_GG
Cuts at: 0 693 954
Size: 693 261
BtgI C'CryG_G
Cuts at: 0 424 954
Size: 424 530
BtsI GCAGTG_nn'
Cuts at: 0 113 614 954
Size: 113 501 340
Fragments arranged by size:
501 340 113
DraI TTT'AAA
Cuts at: 0 828 954
Size: 828 126
EarI CTCTTCn'nnn_
Cuts at: 0 439 586 769 895 916 954
Size: 439 147 183 126 21 38
Fragments arranged by size:
439 183 147 126 38 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 438 876 940 954
Size: 420 18 438 64 14
Fragments arranged by size:
438 420 64 18 14
EcoO109I rG'GnC_Cy
Cuts at: 0 188 954
Size: 188 766
MslI CAynn'nnrTG
Cuts at: 0 634 954
Size: 634 320
NcoI C'CATG_G
Cuts at: 0 424 954
Size: 424 530
PflMI CCAn_nnn'nTGG
Cuts at: 0 323 954
Size: 323 631
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 915 947 954
Size: 915 32 7
Fragments arranged by size:
915 32 7
PstI C_TGCA'G
Cuts at: 0 209 281 804 904 954
Size: 209 72 523 100 50
Fragments arranged by size:
523 209 100 72 50
SapI GCTCTTCn'nnn_
Cuts at: 0 439 769 895 954
Size: 439 330 126 59
Fragments arranged by size:
439 330 126 59
SfcI C'TryA_G
Cuts at: 0 205 277 443 800 900 954
Size: 205 72 166 357 100 54
Fragments arranged by size:
357 205 166 100 72 54
SmlI C'TyrA_G
Cuts at: 0 936 954
Size: 936 18
StyI C'CwwG_G
Cuts at: 0 183 424 529 954
Size: 183 241 105 425
Fragments arranged by size:
425 241 183 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 126 391 404 825 954
Size: 126 265 13 421 129
Fragments arranged by size:
421 265 129 126 13
TatI w'GTAC_w
Cuts at: 0 273 954
Size: 273 681
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 17 49 549 581 954
Size: 17 32 500 32 373
Fragments arranged by size:
500 373 32 32 17
Enzymes that do cut:
AcuI AlfI AlwNI ApoI AvrII BanII BbsI BmrI
BpmI BpuEI BsaI BsaXI BseRI BsgI BsmI Bsp1286I
BspMI BsrDI BstEII BstXI BstYI Bsu36I BtgI BtsI
DraI EarI Eco57MI EcoO109I MslI NcoI PflMI PpiI
PstI SapI SfcI SmlI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AseI
AsiSI AvaI BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI
CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI