Restriction map for pF1KE0837
 (Linear) (Six-base) MAPSORT of: KIEE0837.seq  Check: 7455  from: 1  to: 954



KIEE0837.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     668     954 
   Size:        668     286
 
AlwNI CAG_nnn'CTG

Cuts at:      0     445     954 
   Size:        445     509
 
ApoI r'AATT_y

Cuts at:      0     164     450     931     954 
   Size:        164     286     481      23

  Fragments arranged by size: 

                 481     286     164      23
 
BanII G_rGCy'C

Cuts at:      0     699     954 
   Size:        699     255
 
BbvCI CC'TCA_GC

Cuts at:      0     307     954 
   Size:        307     647
 
BfrBI ATG'CAT

Cuts at:      0      73     883     954 
   Size:         73     810      71

  Fragments arranged by size: 

                 810      73      71
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     111     412     868     954 
   Size:        111     301     456      86

  Fragments arranged by size: 

                 456     301     111      86
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     750     954 
   Size:        750     204
 
Bpu10I CC'TnA_GC

Cuts at:      0     307     954 
   Size:        307     647
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     819     954 
   Size:        819     135
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     662     954 
   Size:        662     292
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     715     954 
   Size:        715     239
 
BseYI C'CCAG_C

Cuts at:      0     673     954 
   Size:        673     281
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     684     954 
   Size:         34     650     270

  Fragments arranged by size: 

                 650     270      34
 
BsmI GAATG_Cn'

Cuts at:      0      73      75     647     954 
   Size:         73       2     572     307

  Fragments arranged by size: 

                 572     307      73       2
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     778     954 
   Size:        778     176
 
Bsp1286I G_dGCh'C

Cuts at:      0     699     954 
   Size:        699     255
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     310     954 
   Size:        310     644
 
BsrDI GCAATG_nn'

Cuts at:      0     755     954 
   Size:        755     199
 
BstYI r'GATC_y

Cuts at:      0      59     954 
   Size:         59     895
 
DraIII CAC_nnn'GTG

Cuts at:      0     662     954 
   Size:        662     292
 
EaeI y'GGCC_r

Cuts at:      0     152     954 
   Size:        152     802
 
EarI CTCTTCn'nnn_

Cuts at:      0     106     954 
   Size:        106     848
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     750     954 
   Size:        750     204
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      21     954 
   Size:         21     933
 
MscI TGG'CCA

Cuts at:      0     154     954 
   Size:        154     800
 
NsiI A_TGCA'T

Cuts at:      0      75     885     954 
   Size:         75     810      69

  Fragments arranged by size: 

                 810      75      69
 
NspI r_CATG'y

Cuts at:      0     538     883     954 
   Size:        538     345      71

  Fragments arranged by size: 

                 538     345      71
 
PfoI T'CCnGG_A

Cuts at:      0     391     954 
   Size:        391     563
 
SmlI C'TyrA_G

Cuts at:      0     834     954 
   Size:        834     120
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     362     954 
   Size:        362     592
 
Tth111I GACn'n_nGTC

Cuts at:      0     103     954 
   Size:        103     851
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     356     954 
   Size:        356     598
 
XmnI GAAnn'nnTTC

Cuts at:      0      73     954 
   Size:         73     881
 

 Enzymes that do cut:

     AlfI    AlwNI     ApoI    BanII    BbvCI    BfrBI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BseRI    BseYI     BsgI     BsmI    BsmBI
 Bsp1286I    BspMI    BsrDI    BstYI   DraIII     EaeI     EarI  Eco57MI
     MmeI     MscI     NsiI     NspI     PfoI     SmlI    TaqII  Tth111I
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BsiEI  BsiHKAI    BsiWI    BspEI    BspHI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI     XbaI     XhoI
     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com