(Linear) (Six-base) MAPSORT of: KIEE0837.seq Check: 7455 from: 1 to: 954 KIEE0837.orf With 182 enzymes: SgfI * April 24, 2009 13:18 .. AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 668 954 Size: 668 286 AlwNI CAG_nnn'CTG Cuts at: 0 445 954 Size: 445 509 ApoI r'AATT_y Cuts at: 0 164 450 931 954 Size: 164 286 481 23 Fragments arranged by size: 481 286 164 23 BanII G_rGCy'C Cuts at: 0 699 954 Size: 699 255 BbvCI CC'TCA_GC Cuts at: 0 307 954 Size: 307 647 BfrBI ATG'CAT Cuts at: 0 73 883 954 Size: 73 810 71 Fragments arranged by size: 810 73 71 BmrI ACTGGGnnnn_n' Cuts at: 0 111 412 868 954 Size: 111 301 456 86 Fragments arranged by size: 456 301 111 86 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 750 954 Size: 750 204 Bpu10I CC'TnA_GC Cuts at: 0 307 954 Size: 307 647 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 819 954 Size: 819 135 BsaI GGTCTCn'nnnn_ Cuts at: 0 662 954 Size: 662 292 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 715 954 Size: 715 239 BseYI C'CCAG_C Cuts at: 0 673 954 Size: 673 281 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 684 954 Size: 34 650 270 Fragments arranged by size: 650 270 34 BsmI GAATG_Cn' Cuts at: 0 73 75 647 954 Size: 73 2 572 307 Fragments arranged by size: 572 307 73 2 BsmBI CGTCTCn'nnnn_ Cuts at: 0 778 954 Size: 778 176 Bsp1286I G_dGCh'C Cuts at: 0 699 954 Size: 699 255 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 310 954 Size: 310 644 BsrDI GCAATG_nn' Cuts at: 0 755 954 Size: 755 199 BstYI r'GATC_y Cuts at: 0 59 954 Size: 59 895 DraIII CAC_nnn'GTG Cuts at: 0 662 954 Size: 662 292 EaeI y'GGCC_r Cuts at: 0 152 954 Size: 152 802 EarI CTCTTCn'nnn_ Cuts at: 0 106 954 Size: 106 848 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 750 954 Size: 750 204 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 21 954 Size: 21 933 MscI TGG'CCA Cuts at: 0 154 954 Size: 154 800 NsiI A_TGCA'T Cuts at: 0 75 885 954 Size: 75 810 69 Fragments arranged by size: 810 75 69 NspI r_CATG'y Cuts at: 0 538 883 954 Size: 538 345 71 Fragments arranged by size: 538 345 71 PfoI T'CCnGG_A Cuts at: 0 391 954 Size: 391 563 SmlI C'TyrA_G Cuts at: 0 834 954 Size: 834 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 362 954 Size: 362 592 Tth111I GACn'n_nGTC Cuts at: 0 103 954 Size: 103 851 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 356 954 Size: 356 598 XmnI GAAnn'nnTTC Cuts at: 0 73 954 Size: 73 881 Enzymes that do cut: AlfI AlwNI ApoI BanII BbvCI BfrBI BmrI BpmI Bpu10I BpuEI BsaI BseRI BseYI BsgI BsmI BsmBI Bsp1286I BspMI BsrDI BstYI DraIII EaeI EarI Eco57MI MmeI MscI NsiI NspI PfoI SmlI TaqII Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TatI TstI XbaI XhoI XmaI ZraI