(Linear) (Six-base) MAPSORT of: KIEE0839.seq  Check: 9561  from: 1  to: 936



KIEE0839.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     901     936 
   Size:        385     516      35

  Fragments arranged by size: 

                 516     385      35
 
AfeI AGC'GCT

Cuts at:      0     762     936 
   Size:        762     174
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     253     936 
   Size:        253     683
 
AlwNI CAG_nnn'CTG

Cuts at:      0     167     936 
   Size:        167     769
 
ApoI r'AATT_y

Cuts at:      0     502     518     936 
   Size:        502      16     418

  Fragments arranged by size: 

                 502     418      16
 
BclI T'GATC_A

Cuts at:      0     143     701     936 
   Size:        143     558     235

  Fragments arranged by size: 

                 558     235     143
 
BglI GCCn_nnn'nGGC

Cuts at:      0     694     936 
   Size:        694     242
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     659     730     936 
   Size:        659      71     206

  Fragments arranged by size: 

                 659     206      71
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     936 
   Size:        144     792
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     936 
   Size:        310     626
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     653     936 
   Size:        653     283
 
BsaBI GATnn'nnATC

Cuts at:      0     133     936 
   Size:        133     803
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     706     709     936 
   Size:        706       3     227

  Fragments arranged by size: 

                 706     227       3
 
BspHI T'CATG_A

Cuts at:      0     480     936 
   Size:        480     456
 
Bsu36I CC'TnA_GG

Cuts at:      0     773     936 
   Size:        773     163
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     574     936 
   Size:        574     362
 
BtsI GCAGTG_nn'

Cuts at:      0     704     936 
   Size:        704     232
 
EaeI y'GGCC_r

Cuts at:      0     152     936 
   Size:        152     784
 
EarI CTCTTCn'nnn_

Cuts at:      0     108     936 
   Size:        108     828
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     385     901     936 
   Size:        144     241     516      35

  Fragments arranged by size: 

                 516     241     144      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0     407     936 
   Size:        407     529
 
HaeII r_GCGC'y

Cuts at:      0     764     936 
   Size:        764     172
 
HincII GTy'rAC

Cuts at:      0      90     542     936 
   Size:         90     452     394

  Fragments arranged by size: 

                 452     394      90
 
HindIII A'AGCT_T

Cuts at:      0     872     936 
   Size:        872      64
 
HpaI GTT'AAC

Cuts at:      0      90     542     936 
   Size:         90     452     394

  Fragments arranged by size: 

                 452     394      90
 
MscI TGG'CCA

Cuts at:      0     154     936 
   Size:        154     782
 
NdeI CA'TA_TG

Cuts at:      0     225     936 
   Size:        225     711
 
NspI r_CATG'y

Cuts at:      0     646     936 
   Size:        646     290
 
PpuMI rG'GwC_Cy

Cuts at:      0     407     936 
   Size:        407     529
 
SfcI C'TryA_G

Cuts at:      0     647     936 
   Size:        647     289
 
SmlI C'TyrA_G

Cuts at:      0     325     936 
   Size:        325     611
 
SpeI A'CTAG_T

Cuts at:      0      84     936 
   Size:         84     852
 
SphI G_CATG'C

Cuts at:      0     646     936 
   Size:        646     290
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     362     936 
   Size:        362     574
 
TatI w'GTAC_w

Cuts at:      0      81     667     728     936 
   Size:         81     586      61     208

  Fragments arranged by size: 

                 586     208      81      61
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     770     802     936 
   Size:        770      32     134

  Fragments arranged by size: 

                 770     134      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     356     936 
   Size:        356     580
 
XmnI GAAnn'nnTTC

Cuts at:      0     875     904     936 
   Size:        875      29      32

  Fragments arranged by size: 

                 875      32      29
 

 Enzymes that do cut:

     AcuI     AfeI     AlfI    AlwNI     ApoI     BclI     BglI     BmrI
     BpmI    BpuEI     BsaI    BsaBI    BseRI    BspHI   Bsu36I    BtgZI
     BtsI     EaeI     EarI  Eco57MI EcoO109I    HaeII   HincII  HindIII
     HpaI     MscI     NdeI     NspI    PpuMI     SfcI     SmlI     SpeI
     SphI    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I    BsaAI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I     KasI     KpnI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SrfI     SspI     StuI     StyI
     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI