(Linear) (Six-base) MAPSORT of: KIEE0840.seq  Check: 1713  from: 1  to: 1035



KIEE0840.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     334    1035 
   Size:        334     701
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     246    1035 
   Size:        246     789
 
ApoI r'AATT_y

Cuts at:      0     449    1014    1035 
   Size:        449     565      21

  Fragments arranged by size: 

                 565     449      21
 
AseI AT'TA_AT

Cuts at:      0     835    1035 
   Size:        835     200
 
AvaI C'yCGr_G

Cuts at:      0      92    1035 
   Size:         92     943
 
BamHI G'GATC_C

Cuts at:      0      88     470    1035 
   Size:         88     382     565

  Fragments arranged by size: 

                 565     382      88
 
BglI GCCn_nnn'nGGC

Cuts at:      0     220     745    1035 
   Size:        220     525     290

  Fragments arranged by size: 

                 525     290     220
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     600    1035 
   Size:        600     435
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     550    1035 
   Size:        550     485
 
BsaBI GATnn'nnATC

Cuts at:      0     166    1035 
   Size:        166     869
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     297     327    1035 
   Size:        297      30     708

  Fragments arranged by size: 

                 708     297      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     318    1035 
   Size:        318     717
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     172    1035 
   Size:         52     120     863

  Fragments arranged by size: 

                 863     120      52
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     334    1035 
   Size:        334     701
 
BsrDI GCAATG_nn'

Cuts at:      0     392    1035 
   Size:        392     643
 
BsrGI T'GTAC_A

Cuts at:      0     104     485    1035 
   Size:        104     381     550

  Fragments arranged by size: 

                 550     381     104
 
BstEII G'GTnAC_C

Cuts at:      0     812    1035 
   Size:        812     223
 
BstYI r'GATC_y

Cuts at:      0      88     470    1035 
   Size:         88     382     565

  Fragments arranged by size: 

                 565     382      88
 
BtgI C'CryG_G

Cuts at:      0     946    1035 
   Size:        946      89
 
DraI TTT'AAA

Cuts at:      0     992    1035 
   Size:        992      43
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     412    1035 
   Size:        412     623
 
EaeI y'GGCC_r

Cuts at:      0     746    1035 
   Size:        746     289
 
EarI CTCTTCn'nnn_

Cuts at:      0     357    1035 
   Size:        357     678
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     246     550    1035 
   Size:        246     304     485

  Fragments arranged by size: 

                 485     304     246
 
EcoO109I rG'GnC_Cy

Cuts at:      0      59    1035 
   Size:         59     976
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     523     555     595     627    1035 
   Size:        523      32      40      32     408

  Fragments arranged by size: 

                 523     408      40      32      32
 
HincII GTy'rAC

Cuts at:      0     599    1035 
   Size:        599     436
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     220    1035 
   Size:        220     815
 
MscI TGG'CCA

Cuts at:      0     748    1035 
   Size:        748     287
 
MslI CAynn'nnrTG

Cuts at:      0     460    1035 
   Size:        460     575
 
MspA1I CmG'CkG

Cuts at:      0      36     186     491    1035 
   Size:         36     150     305     544

  Fragments arranged by size: 

                 544     305     150      36
 
NcoI C'CATG_G

Cuts at:      0     946    1035 
   Size:        946      89
 
PasI CC'CwG_GG

Cuts at:      0     777    1035 
   Size:        777     258
 
PfoI T'CCnGG_A

Cuts at:      0     466    1035 
   Size:        466     569
 
PpuMI rG'GwC_Cy

Cuts at:      0      59    1035 
   Size:         59     976
 
PsiI TTA'TAA

Cuts at:      0     859     905    1035 
   Size:        859      46     130

  Fragments arranged by size: 

                 859     130      46
 
PvuII CAG'CTG

Cuts at:      0      36     186    1035 
   Size:         36     150     849

  Fragments arranged by size: 

                 849     150      36
 
SmaI CCC'GGG

Cuts at:      0      94    1035 
   Size:         94     941
 
StyI C'CwwG_G

Cuts at:      0     946    1035 
   Size:        946      89
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     232     582    1035 
   Size:        232     350     453

  Fragments arranged by size: 

                 453     350     232
 
TatI w'GTAC_w

Cuts at:      0     104     313     485    1035 
   Size:        104     209     172     550

  Fragments arranged by size: 

                 550     209     172     104
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      80     112    1035 
   Size:         80      32     923

  Fragments arranged by size: 

                 923      80      32
 
XmaI C'CCGG_G

Cuts at:      0      92    1035 
   Size:         92     943
 

 Enzymes that do cut:

     AarI     AcuI     ApoI     AseI     AvaI    BamHI     BglI     BmrI
     BpmI    BsaBI    BsaXI    BseRI     BsgI    BspMI    BsrDI    BsrGI
   BstEII    BstYI     BtgI     DraI     DrdI     EaeI     EarI  Eco57MI
 EcoO109I    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NcoI
     PasI     PfoI    PpuMI     PsiI    PvuII     SmaI     StyI    TaqII
     TatI     TstI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI    AvrII     BaeI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI