(Linear) (Six-base) MAPSORT of: KIEE0841.seq  Check: 1591  from: 1  to: 1026



KIEE0841.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AccI GT'mk_AC

Cuts at:      0     817    1026 
   Size:        817     209
 
AlwNI CAG_nnn'CTG

Cuts at:      0     326    1026 
   Size:        326     700
 
ApoI r'AATT_y

Cuts at:      0     304     446     878    1026 
   Size:        304     142     432     148

  Fragments arranged by size: 

                 432     304     148     142
 
AseI AT'TA_AT

Cuts at:      0     832    1026 
   Size:        832     194
 
AvaI C'yCGr_G

Cuts at:      0      89    1026 
   Size:         89     937
 
BglI GCCn_nnn'nGGC

Cuts at:      0     217    1026 
   Size:        217     809
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     547    1026 
   Size:        547     479
 
BsaAI yAC'GTr

Cuts at:      0     482    1026 
   Size:        482     544
 
BseYI C'CCAG_C

Cuts at:      0     587    1026 
   Size:        587     439
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     169    1026 
   Size:         49     120     857

  Fragments arranged by size: 

                 857     120      49
 
BsiHKAI G_wGCw'C

Cuts at:      0     546    1026 
   Size:        546     480
 
Bsp1286I G_dGCh'C

Cuts at:      0     546    1026 
   Size:        546     480
 
BsrDI GCAATG_nn'

Cuts at:      0     206    1026 
   Size:        206     820
 
BsrGI T'GTAC_A

Cuts at:      0     101    1026 
   Size:        101     925
 
BstEII G'GTnAC_C

Cuts at:      0     809    1026 
   Size:        809     217
 
BstYI r'GATC_y

Cuts at:      0      55    1026 
   Size:         55     971
 
BstZ17I GTA'TAC

Cuts at:      0     818    1026 
   Size:        818     208
 
DraI TTT'AAA

Cuts at:      0     989    1026 
   Size:        989      37
 
EaeI y'GGCC_r

Cuts at:      0     743    1026 
   Size:        743     283
 
EarI CTCTTCn'nnn_

Cuts at:      0     320     354    1026 
   Size:        320      34     672

  Fragments arranged by size: 

                 672     320      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     547    1026 
   Size:        547     479
 
EcoO109I rG'GnC_Cy

Cuts at:      0      86    1026 
   Size:         86     940
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     459     491     915     947    1026 
   Size:        459      32     424      32      79

  Fragments arranged by size: 

                 459     424      79      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     868    1026 
   Size:        217     651     158

  Fragments arranged by size: 

                 651     217     158
 
MscI TGG'CCA

Cuts at:      0     745    1026 
   Size:        745     281
 
MslI CAynn'nnrTG

Cuts at:      0     457    1026 
   Size:        457     569
 
MspA1I CmG'CkG

Cuts at:      0     183     488    1026 
   Size:        183     305     538

  Fragments arranged by size: 

                 538     305     183
 
PasI CC'CwG_GG

Cuts at:      0     774    1026 
   Size:        774     252
 
PfoI T'CCnGG_A

Cuts at:      0     463    1026 
   Size:        463     563
 
PpuMI rG'GwC_Cy

Cuts at:      0      86    1026 
   Size:         86     940
 
PsiI TTA'TAA

Cuts at:      0     731     902    1026 
   Size:        731     171     124

  Fragments arranged by size: 

                 731     171     124
 
PvuII CAG'CTG

Cuts at:      0     183    1026 
   Size:        183     843
 
SanDI GG'GwC_CC

Cuts at:      0      86    1026 
   Size:         86     940
 
SmaI CCC'GGG

Cuts at:      0      91    1026 
   Size:         91     935
 
SpeI A'CTAG_T

Cuts at:      0     697    1026 
   Size:        697     329
 
StyI C'CwwG_G

Cuts at:      0     943    1026 
   Size:        943      83
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     579    1026 
   Size:        579     447
 
TatI w'GTAC_w

Cuts at:      0     101     310    1026 
   Size:        101     209     716

  Fragments arranged by size: 

                 716     209     101
 
XmaI C'CCGG_G

Cuts at:      0      89    1026 
   Size:         89     937
 

 Enzymes that do cut:

     AccI    AlwNI     ApoI     AseI     AvaI     BglI     BpmI    BsaAI
    BseYI     BsgI  BsiHKAI Bsp1286I    BsrDI    BsrGI   BstEII    BstYI
  BstZ17I     DraI     EaeI     EarI  Eco57MI EcoO109I    Hin4I     MmeI
     MscI     MslI   MspA1I     PasI     PfoI    PpuMI     PsiI    PvuII
    SanDI     SmaI     SpeI     StyI    TaqII     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI    BstXI   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI