Restriction map for
pF1KE0845
(Linear) (Six-base) MAPSORT of: KIEE0845.seq Check: 9819 from: 1 to: 942
KIEE0845.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 691 942
Size: 202 489 251
Fragments arranged by size:
489 251 202
AflII C'TTAA_G
Cuts at: 0 772 942
Size: 772 170
AleI CACnn'nnGTG
Cuts at: 0 16 942
Size: 16 926
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 543 575 942
Size: 543 32 367
Fragments arranged by size:
543 367 32
AlwNI CAG_nnn'CTG
Cuts at: 0 60 942
Size: 60 882
ApoI r'AATT_y
Cuts at: 0 232 786 942
Size: 232 554 156
Fragments arranged by size:
554 232 156
BanII G_rGCy'C
Cuts at: 0 455 942
Size: 455 487
BbsI GAAGACnn'nnnn_
Cuts at: 0 198 942
Size: 198 744
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 489 824 942
Size: 428 61 335 118
Fragments arranged by size:
428 335 118 61
BsaBI GATnn'nnATC
Cuts at: 0 331 942
Size: 331 611
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 401 431 942
Size: 401 30 511
Fragments arranged by size:
511 401 30
BsiHKAI G_wGCw'C
Cuts at: 0 83 894 942
Size: 83 811 48
Fragments arranged by size:
811 83 48
Bsp1286I G_dGCh'C
Cuts at: 0 83 455 894 942
Size: 83 372 439 48
Fragments arranged by size:
439 372 83 48
BspHI T'CATG_A
Cuts at: 0 398 942
Size: 398 544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 577 942
Size: 577 365
BstYI r'GATC_y
Cuts at: 0 674 942
Size: 674 268
BtsI GCAGTG_nn'
Cuts at: 0 338 942
Size: 338 604
EaeI y'GGCC_r
Cuts at: 0 2 942
Size: 2 940
EarI CTCTTCn'nnn_
Cuts at: 0 88 942
Size: 88 854
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 428 489 691 824 942
Size: 202 226 61 202 133 118
Fragments arranged by size:
226 202 202 133 118 61
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 635 942
Size: 635 307
HincII GTy'rAC
Cuts at: 0 243 942
Size: 243 699
HindIII A'AGCT_T
Cuts at: 0 701 942
Size: 701 241
MscI TGG'CCA
Cuts at: 0 4 942
Size: 4 938
MslI CAynn'nnrTG
Cuts at: 0 16 343 832 942
Size: 16 327 489 110
Fragments arranged by size:
489 327 110 16
MspA1I CmG'CkG
Cuts at: 0 475 942
Size: 475 467
NdeI CA'TA_TG
Cuts at: 0 918 942
Size: 918 24
NspI r_CATG'y
Cuts at: 0 608 716 942
Size: 608 108 226
Fragments arranged by size:
608 226 108
PstI C_TGCA'G
Cuts at: 0 911 942
Size: 911 31
PvuII CAG'CTG
Cuts at: 0 475 942
Size: 475 467
SapI GCTCTTCn'nnn_
Cuts at: 0 88 942
Size: 88 854
SfcI C'TryA_G
Cuts at: 0 861 907 942
Size: 861 46 35
Fragments arranged by size:
861 46 35
SmlI C'TyrA_G
Cuts at: 0 772 942
Size: 772 170
StyI C'CwwG_G
Cuts at: 0 14 942
Size: 14 928
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 180 942
Size: 180 762
TatI w'GTAC_w
Cuts at: 0 170 666 942
Size: 170 496 276
Fragments arranged by size:
496 276 170
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 247 279 401 433 942
Size: 247 32 122 32 509
Fragments arranged by size:
509 247 122 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 12 942
Size: 12 930
Enzymes that do cut:
AcuI AflII AleI AloI AlwNI ApoI BanII BbsI
BpmI BsaBI BsaXI BsiHKAI Bsp1286I BspHI BspMI BstYI
BtsI EaeI EarI Eco57MI FalI HincII HindIII MscI
MslI MspA1I NdeI NspI PstI PvuII SapI SfcI
SmlI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI