Restriction map for
pF1KE0858
(Linear) (Six-base) MAPSORT of: KIEE0858.seq Check: 2509 from: 1 to: 945
KIEE0858.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 433 945
Size: 433 512
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 716 802 855 913 945
Size: 297 419 86 53 58 32
Fragments arranged by size:
419 297 86 58 53 32
AfeI AGC'GCT
Cuts at: 0 268 945
Size: 268 677
AhdI GACnn_n'nnGTC
Cuts at: 0 255 945
Size: 255 690
AleI CACnn'nnGTG
Cuts at: 0 22 742 945
Size: 22 720 203
Fragments arranged by size:
720 203 22
ApaLI G'TGCA_C
Cuts at: 0 751 945
Size: 751 194
ApoI r'AATT_y
Cuts at: 0 31 945
Size: 31 914
BbsI GAAGACnn'nnnn_
Cuts at: 0 916 945
Size: 916 29
BbvCI CC'TCA_GC
Cuts at: 0 856 945
Size: 856 89
BglI GCCn_nnn'nGGC
Cuts at: 0 283 416 434 945
Size: 283 133 18 511
Fragments arranged by size:
511 283 133 18
Bme1580I G_kGCm'C
Cuts at: 0 195 755 945
Size: 195 560 190
Fragments arranged by size:
560 195 190
BmrI ACTGGGnnnn_n'
Cuts at: 0 792 945
Size: 792 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 168 945
Size: 52 116 777
Fragments arranged by size:
777 116 52
Bpu10I CC'TnA_GC
Cuts at: 0 856 945
Size: 856 89
BsaAI yAC'GTr
Cuts at: 0 369 540 945
Size: 369 171 405
Fragments arranged by size:
405 369 171
BsaBI GATnn'nnATC
Cuts at: 0 826 945
Size: 826 119
BsaWI w'CCGG_w
Cuts at: 0 804 945
Size: 804 141
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4 34 560 590 945
Size: 4 30 526 30 355
Fragments arranged by size:
526 355 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 628 637 945
Size: 628 9 308
Fragments arranged by size:
628 308 9
BseYI C'CCAG_C
Cuts at: 0 117 263 624 945
Size: 117 146 361 321
Fragments arranged by size:
361 321 146 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 945
Size: 405 540
BsiEI CG_ry'CG
Cuts at: 0 363 945
Size: 363 582
BsiHKAI G_wGCw'C
Cuts at: 0 404 755 945
Size: 404 351 190
Fragments arranged by size:
404 351 190
Bsp1286I G_dGCh'C
Cuts at: 0 195 404 755 945
Size: 195 209 351 190
Fragments arranged by size:
351 209 195 190
BspEI T'CCGG_A
Cuts at: 0 804 945
Size: 804 141
BspHI T'CATG_A
Cuts at: 0 404 945
Size: 404 541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 260 433 945
Size: 134 126 173 512
Fragments arranged by size:
512 173 134 126
BstXI CCAn_nnnn'nTGG
Cuts at: 0 597 945
Size: 597 348
BstYI r'GATC_y
Cuts at: 0 209 518 683 945
Size: 209 309 165 262
Fragments arranged by size:
309 262 209 165
Bsu36I CC'TnA_GG
Cuts at: 0 877 945
Size: 877 68
BtgI C'CryG_G
Cuts at: 0 221 945
Size: 221 724
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 258 945
Size: 258 687
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 533 568 945
Size: 533 35 377
Fragments arranged by size:
533 377 35
EaeI y'GGCC_r
Cuts at: 0 137 224 245 371 482 587 668 945
Size: 137 87 21 126 111 105 81 277
Fragments arranged by size:
277 137 126 111 105 87 81 21
EarI CTCTTCn'nnn_
Cuts at: 0 85 187 945
Size: 85 102 758
Fragments arranged by size:
758 102 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 168 297 716 802 855 913 945
Size: 52 116 129 419 86 53 58 32
Fragments arranged by size:
419 129 116 86 58 53 52 32
EcoO109I rG'GnC_Cy
Cuts at: 0 793 945
Size: 793 152
EcoRI G'AATT_C
Cuts at: 0 31 945
Size: 31 914
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 933 945
Size: 933 12
HaeII r_GCGC'y
Cuts at: 0 270 945
Size: 270 675
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 945
Size: 241 32 672
Fragments arranged by size:
672 241 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 945
Size: 682 263
MscI TGG'CCA
Cuts at: 0 139 226 373 589 945
Size: 139 87 147 216 356
Fragments arranged by size:
356 216 147 139 87
MslI CAynn'nnrTG
Cuts at: 0 22 172 349 466 742 820 945
Size: 22 150 177 117 276 78 125
Fragments arranged by size:
276 177 150 125 117 78 22
MspA1I CmG'CkG
Cuts at: 0 72 945
Size: 72 873
NspI r_CATG'y
Cuts at: 0 171 243 945
Size: 171 72 702
Fragments arranged by size:
702 171 72
PflMI CCAn_nnn'nTGG
Cuts at: 0 146 945
Size: 146 799
PmlI CAC'GTG
Cuts at: 0 540 945
Size: 540 405
PpuMI rG'GwC_Cy
Cuts at: 0 793 945
Size: 793 152
PvuII CAG'CTG
Cuts at: 0 72 945
Size: 72 873
SapI GCTCTTCn'nnn_
Cuts at: 0 85 945
Size: 85 860
SphI G_CATG'C
Cuts at: 0 243 945
Size: 243 702
StuI AGG'CCT
Cuts at: 0 712 945
Size: 712 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 277 343 945
Size: 277 66 602
Fragments arranged by size:
602 277 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 560 592 945
Size: 560 32 353
Fragments arranged by size:
560 353 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 279 945
Size: 279 666
Enzymes that do cut:
AarI AcuI AfeI AhdI AleI ApaLI ApoI BbsI
BbvCI BglI Bme1580I BmrI BpmI Bpu10I BsaAI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BstXI BstYI Bsu36I BtgI BtgZI
CspCI EaeI EarI Eco57MI EcoO109I EcoRI FalI HaeII
Hin4I MmeI MscI MslI MspA1I NspI PflMI PmlI
PpuMI PvuII SapI SphI StuI TaqII TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AlfI
AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaI
BsaHI BsiWI BsmI BsmBI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I BtsI ClaI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI StyI SwaI TatI Tth111I XbaI
XhoI XmaI XmnI ZraI