Restriction map for
pF1KE0859
(Linear) (Six-base) MAPSORT of: KIEE0859.seq Check: 1494 from: 1 to: 987
KIEE0859.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 560 730 987
Size: 560 170 257
Fragments arranged by size:
560 257 170
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 577 716 802 896 987
Size: 42 535 139 86 94 91
Fragments arranged by size:
535 139 94 91 86 42
AflII C'TTAA_G
Cuts at: 0 679 987
Size: 679 308
AleI CACnn'nnGTG
Cuts at: 0 22 987
Size: 22 965
AlwNI CAG_nnn'CTG
Cuts at: 0 614 987
Size: 614 373
ApoI r'AATT_y
Cuts at: 0 12 987
Size: 12 975
BbsI GAAGACnn'nnnn_
Cuts at: 0 72 84 987
Size: 72 12 903
Fragments arranged by size:
903 72 12
BbvCI CC'TCA_GC
Cuts at: 0 690 987
Size: 690 297
BciVI GTATCCnnnnn_n'
Cuts at: 0 436 987
Size: 436 551
BmrI ACTGGGnnnn_n'
Cuts at: 0 457 987
Size: 457 530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 987
Size: 36 951
Bpu10I CC'TnA_GC
Cuts at: 0 690 987
Size: 690 297
BsaI GGTCTCn'nnnn_
Cuts at: 0 205 259 987
Size: 205 54 728
Fragments arranged by size:
728 205 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 916 946 987
Size: 916 30 41
Fragments arranged by size:
916 41 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 887 987
Size: 552 335 100
Fragments arranged by size:
552 335 100
BspHI T'CATG_A
Cuts at: 0 137 987
Size: 137 850
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 116 223 560 730 987
Size: 116 107 337 170 257
Fragments arranged by size:
337 257 170 116 107
BsrDI GCAATG_nn'
Cuts at: 0 132 275 987
Size: 132 143 712
Fragments arranged by size:
712 143 132
BsrGI T'GTAC_A
Cuts at: 0 867 987
Size: 867 120
Bsu36I CC'TnA_GG
Cuts at: 0 391 987
Size: 391 596
BtgI C'CryG_G
Cuts at: 0 912 987
Size: 912 75
DrdI GACnn_nn'nnGTC
Cuts at: 0 580 987
Size: 580 407
EaeI y'GGCC_r
Cuts at: 0 227 374 467 987
Size: 227 147 93 520
Fragments arranged by size:
520 227 147 93
EarI CTCTTCn'nnn_
Cuts at: 0 310 937 987
Size: 310 627 50
Fragments arranged by size:
627 310 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 42 577 716 802 896 987
Size: 36 6 535 139 86 94 91
Fragments arranged by size:
535 139 94 91 86 36 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 808 987
Size: 808 179
EcoO109I rG'GnC_Cy
Cuts at: 0 419 987
Size: 419 568
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 729 987
Size: 729 258
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 822 854 987
Size: 822 32 133
Fragments arranged by size:
822 133 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 987
Size: 453 534
MscI TGG'CCA
Cuts at: 0 229 376 469 987
Size: 229 147 93 518
Fragments arranged by size:
518 229 147 93
MslI CAynn'nnrTG
Cuts at: 0 22 175 352 826 987
Size: 22 153 177 474 161
Fragments arranged by size:
474 177 161 153 22
MspA1I CmG'CkG
Cuts at: 0 694 987
Size: 694 293
NdeI CA'TA_TG
Cuts at: 0 242 987
Size: 242 745
NspI r_CATG'y
Cuts at: 0 198 987
Size: 198 789
PasI CC'CwG_GG
Cuts at: 0 804 987
Size: 804 183
PsiI TTA'TAA
Cuts at: 0 976 987
Size: 976 11
PvuII CAG'CTG
Cuts at: 0 694 987
Size: 694 293
SapI GCTCTTCn'nnn_
Cuts at: 0 310 987
Size: 310 677
SmlI C'TyrA_G
Cuts at: 0 679 987
Size: 679 308
SphI G_CATG'C
Cuts at: 0 198 987
Size: 198 789
StuI AGG'CCT
Cuts at: 0 712 987
Size: 712 275
StyI C'CwwG_G
Cuts at: 0 459 987
Size: 459 528
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 298 987
Size: 298 689
TatI w'GTAC_w
Cuts at: 0 179 867 987
Size: 179 688 120
Fragments arranged by size:
688 179 120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 150 247 436 987
Size: 150 97 189 551
Fragments arranged by size:
551 189 150 97
Enzymes that do cut:
AarI AcuI AflII AleI AlwNI ApoI BbsI BbvCI
BciVI BmrI BpmI Bpu10I BsaI BsaXI BsgI BspHI
BspMI BsrDI BsrGI Bsu36I BtgI DrdI EaeI EarI
Eco57MI EcoNI EcoO109I FalI Hin4I MmeI MscI MslI
MspA1I NdeI NspI PasI PsiI PvuII SapI SmlI
SphI StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BcgI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII EagI EciI
EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI