Restriction map for
pF1KE0868
(Linear) (Six-base) MAPSORT of: KIEE0868.seq Check: 4965 from: 1 to: 915
KIEE0868.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 700 915
Size: 700 215
AleI CACnn'nnGTG
Cuts at: 0 196 712 915
Size: 196 516 203
Fragments arranged by size:
516 203 196
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 886 915
Size: 27 859 29
Fragments arranged by size:
859 29 27
AlwNI CAG_nnn'CTG
Cuts at: 0 896 915
Size: 896 19
ApoI r'AATT_y
Cuts at: 0 4 915
Size: 4 911
BanI G'GyrC_C
Cuts at: 0 204 915
Size: 204 711
BbsI GAAGACnn'nnnn_
Cuts at: 0 247 915
Size: 247 668
BclI T'GATC_A
Cuts at: 0 83 915
Size: 83 832
BfrBI ATG'CAT
Cuts at: 0 528 915
Size: 528 387
Bme1580I G_kGCm'C
Cuts at: 0 209 757 915
Size: 209 548 158
Fragments arranged by size:
548 209 158
BmrI ACTGGGnnnn_n'
Cuts at: 0 101 301 915
Size: 101 200 614
Fragments arranged by size:
614 200 101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 747 915
Size: 747 168
Bpu10I CC'TnA_GC
Cuts at: 0 485 915
Size: 485 430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 915
Size: 634 281
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 364 915
Size: 364 551
BseYI C'CCAG_C
Cuts at: 0 757 915
Size: 757 158
BsiHKAI G_wGCw'C
Cuts at: 0 500 543 915
Size: 500 43 372
Fragments arranged by size:
500 372 43
Bsp1286I G_dGCh'C
Cuts at: 0 209 500 543 757 915
Size: 209 291 43 214 158
Fragments arranged by size:
291 214 209 158 43
BspHI T'CATG_A
Cuts at: 0 377 555 915
Size: 377 178 360
Fragments arranged by size:
377 360 178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 700 915
Size: 700 215
BsrDI GCAATG_nn'
Cuts at: 0 284 590 915
Size: 284 306 325
Fragments arranged by size:
325 306 284
BsrGI T'GTAC_A
Cuts at: 0 803 915
Size: 803 112
BstAPI GCAn_nnn'nTGC
Cuts at: 0 567 915
Size: 567 348
BstXI CCAn_nnnn'nTGG
Cuts at: 0 273 915
Size: 273 642
BstYI r'GATC_y
Cuts at: 0 653 915
Size: 653 262
Bsu36I CC'TnA_GG
Cuts at: 0 847 915
Size: 847 68
BtgI C'CryG_G
Cuts at: 0 323 915
Size: 323 592
BtsI GCAGTG_nn'
Cuts at: 0 191 203 303 820 915
Size: 191 12 100 517 95
Fragments arranged by size:
517 191 100 95 12
EaeI y'GGCC_r
Cuts at: 0 218 344 669 915
Size: 218 126 325 246
Fragments arranged by size:
325 246 218 126
EarI CTCTTCn'nnn_
Cuts at: 0 702 915
Size: 702 213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 747 915
Size: 747 168
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 234 266 372 404 915
Size: 234 32 106 32 511
Fragments arranged by size:
511 234 106 32 32
MscI TGG'CCA
Cuts at: 0 220 346 915
Size: 220 126 569
Fragments arranged by size:
569 220 126
MslI CAynn'nnrTG
Cuts at: 0 109 145 196 271 554 595 712 915
Size: 109 36 51 75 283 41 117 203
Fragments arranged by size:
283 203 117 109 75 51 41 36
NcoI C'CATG_G
Cuts at: 0 323 915
Size: 323 592
NsiI A_TGCA'T
Cuts at: 0 530 915
Size: 530 385
NspI r_CATG'y
Cuts at: 0 532 915
Size: 532 383
PflMI CCAn_nnn'nTGG
Cuts at: 0 216 915
Size: 216 699
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 866 898 915
Size: 866 32 17
Fragments arranged by size:
866 32 17
PstI C_TGCA'G
Cuts at: 0 198 725 915
Size: 198 527 190
Fragments arranged by size:
527 198 190
SapI GCTCTTCn'nnn_
Cuts at: 0 702 915
Size: 702 213
SfcI C'TryA_G
Cuts at: 0 194 229 721 915
Size: 194 35 492 194
Fragments arranged by size:
492 194 194 35
SmlI C'TyrA_G
Cuts at: 0 649 915
Size: 649 266
StyI C'CwwG_G
Cuts at: 0 57 323 915
Size: 57 266 592
Fragments arranged by size:
592 266 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 283 915
Size: 283 632
TatI w'GTAC_w
Cuts at: 0 149 803 915
Size: 149 654 112
Fragments arranged by size:
654 149 112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 252 664 915
Size: 252 412 251
Fragments arranged by size:
412 252 251
Enzymes that do cut:
AarI AleI AloI AlwNI ApoI BanI BbsI BclI
BfrBI Bme1580I BmrI BpmI Bpu10I BpuEI BseRI BseYI
BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstXI
BstYI Bsu36I BtgI BtsI EaeI EarI Eco57MI Hin4I
MscI MslI NcoI NsiI NspI PflMI PsrI PstI
SapI SfcI SmlI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BciVI BglI BglII BlpI BmgBI BmtI BplI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BsrBI BsrFI BssHII BssSI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI