Restriction map for
pF1KE0872
(Linear) (Six-base) MAPSORT of: KIEE0872.seq Check: 5792 from: 1 to: 990
KIEE0872.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 0 884 990
Size: 884 106
Acc65I G'GTAC_C
Cuts at: 0 419 990
Size: 419 571
AflIII A'CryG_T
Cuts at: 0 772 990
Size: 772 218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 633 990
Size: 633 357
ApaI G_GGCC'C
Cuts at: 0 566 683 990
Size: 566 117 307
Fragments arranged by size:
566 307 117
BanI G'GyrC_C
Cuts at: 0 419 990
Size: 419 571
BanII G_rGCy'C
Cuts at: 0 566 683 838 990
Size: 566 117 155 152
Fragments arranged by size:
566 155 152 117
BbsI GAAGACnn'nnnn_
Cuts at: 0 919 990
Size: 919 71
BfrBI ATG'CAT
Cuts at: 0 72 990
Size: 72 918
BglII A'GATC_T
Cuts at: 0 263 990
Size: 263 727
Bme1580I G_kGCm'C
Cuts at: 0 566 683 990
Size: 566 117 307
Fragments arranged by size:
566 307 117
BmrI ACTGGGnnnn_n'
Cuts at: 0 382 473 990
Size: 382 91 517
Fragments arranged by size:
517 382 91
BsaBI GATnn'nnATC
Cuts at: 0 670 990
Size: 670 320
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 173 203 262 292 436 466 990
Size: 173 30 59 30 144 30 524
Fragments arranged by size:
524 173 144 59 30 30 30
BseYI C'CCAG_C
Cuts at: 0 497 852 990
Size: 497 355 138
Fragments arranged by size:
497 355 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 990
Size: 215 775
BsmI GAATG_Cn'
Cuts at: 0 866 990
Size: 866 124
Bsp1286I G_dGCh'C
Cuts at: 0 566 683 838 990
Size: 566 117 155 152
Fragments arranged by size:
566 155 152 117
BspHI T'CATG_A
Cuts at: 0 458 990
Size: 458 532
BsrDI GCAATG_nn'
Cuts at: 0 899 990
Size: 899 91
BsrGI T'GTAC_A
Cuts at: 0 230 990
Size: 230 760
BstBI TT'CG_AA
Cuts at: 0 930 990
Size: 930 60
BstYI r'GATC_y
Cuts at: 0 263 700 990
Size: 263 437 290
Fragments arranged by size:
437 290 263
BstZ17I GTA'TAC
Cuts at: 0 885 990
Size: 885 105
BtgI C'CryG_G
Cuts at: 0 105 990
Size: 105 885
BtsI GCAGTG_nn'
Cuts at: 0 166 203 990
Size: 166 37 787
Fragments arranged by size:
787 166 37
DraIII CAC_nnn'GTG
Cuts at: 0 585 990
Size: 585 405
EaeI y'GGCC_r
Cuts at: 0 425 990
Size: 425 565
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 521 990
Size: 521 469
EcoO109I rG'GnC_Cy
Cuts at: 0 52 680 779 990
Size: 52 628 99 211
Fragments arranged by size:
628 211 99 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 173 205 861 893 990
Size: 173 32 656 32 97
Fragments arranged by size:
656 173 97 32 32
HincII GTy'rAC
Cuts at: 0 974 990
Size: 974 16
HindIII A'AGCT_T
Cuts at: 0 761 990
Size: 761 229
HpaI GTT'AAC
Cuts at: 0 974 990
Size: 974 16
KpnI G_GTAC'C
Cuts at: 0 423 990
Size: 423 567
MscI TGG'CCA
Cuts at: 0 427 990
Size: 427 563
MslI CAynn'nnrTG
Cuts at: 0 226 892 990
Size: 226 666 98
Fragments arranged by size:
666 226 98
NcoI C'CATG_G
Cuts at: 0 105 990
Size: 105 885
NsiI A_TGCA'T
Cuts at: 0 74 990
Size: 74 916
NspI r_CATG'y
Cuts at: 0 297 776 990
Size: 297 479 214
Fragments arranged by size:
479 297 214
PciI A'CATG_T
Cuts at: 0 772 990
Size: 772 218
PflMI CCAn_nnn'nTGG
Cuts at: 0 789 990
Size: 789 201
PpuMI rG'GwC_Cy
Cuts at: 0 52 779 990
Size: 52 727 211
Fragments arranged by size:
727 211 52
PspOMI G'GGCC_C
Cuts at: 0 562 679 990
Size: 562 117 311
Fragments arranged by size:
562 311 117
SanDI GG'GwC_CC
Cuts at: 0 52 779 990
Size: 52 727 211
Fragments arranged by size:
727 211 52
SfcI C'TryA_G
Cuts at: 0 921 990
Size: 921 69
StyI C'CwwG_G
Cuts at: 0 105 990
Size: 105 885
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 240 990
Size: 240 750
TatI w'GTAC_w
Cuts at: 0 230 990
Size: 230 760
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 436 468 990
Size: 436 32 522
Fragments arranged by size:
522 436 32
XbaI T'CTAG_A
Cuts at: 0 258 990
Size: 258 732
Enzymes that do cut:
AccI Acc65I AflIII AlfI ApaI BanI BanII BbsI
BfrBI BglII Bme1580I BmrI BsaBI BsaXI BseYI BsgI
BsmI Bsp1286I BspHI BsrDI BsrGI BstBI BstYI BstZ17I
BtgI BtsI DraIII EaeI EciI EcoO109I Hin4I HincII
HindIII HpaI KpnI MscI MslI NcoI NsiI NspI
PciI PflMI PpuMI PspOMI SanDI SfcI StyI TaqII
TatI TstI XbaI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BglI BlpI BmgBI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BseRI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstEII BstXI Bsu36I BtgZI ClaI CspCI DraI
DrdI EagI EarI EcoICRI Eco57MI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII KasI MfeI MluI
MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XcmI XhoI XmaI XmnI
ZraI