(Linear) (Six-base) MAPSORT of: KIEE0875.seq  Check: 6178  from: 1  to: 1041



KIEE0875.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

Acc65I G'GTAC_C

Cuts at:      0     847    1041 
   Size:        847     194
 
AleI CACnn'nnGTG

Cuts at:      0     685    1041 
   Size:        685     356
 
ApoI r'AATT_y

Cuts at:      0     121     552     774    1041 
   Size:        121     431     222     267

  Fragments arranged by size: 

                 431     267     222     121
 
BanI G'GyrC_C

Cuts at:      0     847    1041 
   Size:        847     194
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     795    1041 
   Size:        795     246
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     139    1041 
   Size:        139     902
 
BclI T'GATC_A

Cuts at:      0     833    1041 
   Size:        833     208
 
BglII A'GATC_T

Cuts at:      0      58    1041 
   Size:         58     983
 
BlpI GC'TnA_GC

Cuts at:      0     813    1041 
   Size:        813     228
 
Bpu10I CC'TnA_GC

Cuts at:      0     496    1041 
   Size:        496     545
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     628     866    1002    1041 
   Size:        628     238     136      39

  Fragments arranged by size: 

                 628     238     136      39
 
BsaAI yAC'GTr

Cuts at:      0     809    1041 
   Size:        809     232
 
BsaWI w'CCGG_w

Cuts at:      0     541    1041 
   Size:        541     500
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     306    1041 
   Size:        306     735
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     401     406    1041 
   Size:        401       5     635

  Fragments arranged by size: 

                 635     401       5
 
BsiHKAI G_wGCw'C

Cuts at:      0     814    1041 
   Size:        814     227
 
Bsp1286I G_dGCh'C

Cuts at:      0     814    1041 
   Size:        814     227
 
BsrDI GCAATG_nn'

Cuts at:      0     219     413    1041 
   Size:        219     194     628

  Fragments arranged by size: 

                 628     219     194
 
BstYI r'GATC_y

Cuts at:      0      58    1041 
   Size:         58     983
 
BtgI C'CryG_G

Cuts at:      0     683    1041 
   Size:        683     358
 
BtsI GCAGTG_nn'

Cuts at:      0     389    1041 
   Size:        389     652
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     114    1041 
   Size:        114     927
 
EaeI y'GGCC_r

Cuts at:      0     461    1041 
   Size:        461     580
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     499    1041 
   Size:        499     542
 
EcoRI G'AATT_C

Cuts at:      0     774    1041 
   Size:        774     267
 
EcoRV GAT'ATC

Cuts at:      0     300     692    1041 
   Size:        300     392     349

  Fragments arranged by size: 

                 392     349     300
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     473     505    1041 
   Size:        473      32     536

  Fragments arranged by size: 

                 536     473      32
 
HincII GTy'rAC

Cuts at:      0     416    1041 
   Size:        416     625
 
KpnI G_GTAC'C

Cuts at:      0     851    1041 
   Size:        851     190
 
MscI TGG'CCA

Cuts at:      0     463    1041 
   Size:        463     578
 
MslI CAynn'nnrTG

Cuts at:      0     262     685    1041 
   Size:        262     423     356

  Fragments arranged by size: 

                 423     356     262
 
MspA1I CmG'CkG

Cuts at:      0     559    1041 
   Size:        559     482
 
NcoI C'CATG_G

Cuts at:      0     683    1041 
   Size:        683     358
 
NspI r_CATG'y

Cuts at:      0     626    1041 
   Size:        626     415
 
PfoI T'CCnGG_A

Cuts at:      0     293    1041 
   Size:        293     748
 
PmlI CAC'GTG

Cuts at:      0     809    1041 
   Size:        809     232
 
PvuII CAG'CTG

Cuts at:      0     559    1041 
   Size:        559     482
 
SfcI C'TryA_G

Cuts at:      0     975    1041 
   Size:        975      66
 
SmlI C'TyrA_G

Cuts at:      0     643     881     981    1041 
   Size:        643     238     100      60

  Fragments arranged by size: 

                 643     238     100      60
 
SspI AAT'ATT

Cuts at:      0     606     632     675    1015    1041 
   Size:        606      26      43     340      26

  Fragments arranged by size: 

                 606     340      43      26      26
 
StyI C'CwwG_G

Cuts at:      0     683    1041 
   Size:        683     358
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      82     969    1041 
   Size:         82     887      72

  Fragments arranged by size: 

                 887      82      72
 
TatI w'GTAC_w

Cuts at:      0      19     266    1041 
   Size:         19     247     775

  Fragments arranged by size: 

                 775     247      19
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     368     400    1041 
   Size:        368      32     641

  Fragments arranged by size: 

                 641     368      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     125     363     552    1041 
   Size:        125     238     189     489

  Fragments arranged by size: 

                 489     238     189     125
 

 Enzymes that do cut:

   Acc65I     AleI     ApoI     BanI     BbsI    BciVI     BclI    BglII
     BlpI   Bpu10I    BpuEI    BsaAI    BsaWI    BseRI     BsgI  BsiHKAI
 Bsp1286I    BsrDI    BstYI     BtgI     BtsI     DrdI     EaeI    EcoNI
    EcoRI    EcoRV    Hin4I   HincII     KpnI     MscI     MslI   MspA1I
     NcoI     NspI     PfoI     PmlI    PvuII     SfcI     SmlI     SspI
     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI    BfrBI     BglI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI     BsaI    BsaBI    BsaHI    BsaXI    BseYI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     MfeI     MluI     MmeI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI