Restriction map for
pF1KE0876
(Linear) (Six-base) MAPSORT of: KIEE0876.seq Check: 1204 from: 1 to: 1038
KIEE0876.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 70 1038
Size: 70 968
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 545 1036 1038
Size: 545 491 2
Fragments arranged by size:
545 491 2
ApoI r'AATT_y
Cuts at: 0 127 635 1038
Size: 127 508 403
Fragments arranged by size:
508 403 127
AseI AT'TA_AT
Cuts at: 0 779 1038
Size: 779 259
BanII G_rGCy'C
Cuts at: 0 164 421 1038
Size: 164 257 617
Fragments arranged by size:
617 257 164
BbvCI CC'TCA_GC
Cuts at: 0 788 1038
Size: 788 250
BclI T'GATC_A
Cuts at: 0 618 1038
Size: 618 420
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 1038
Size: 148 890
Bpu10I CC'TnA_GC
Cuts at: 0 788 1009 1038
Size: 788 221 29
Fragments arranged by size:
788 221 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 217 1038
Size: 217 821
BseYI C'CCAG_C
Cuts at: 0 71 1038
Size: 71 967
BsiHKAI G_wGCw'C
Cuts at: 0 164 390 1038
Size: 164 226 648
Fragments arranged by size:
648 226 164
Bsp1286I G_dGCh'C
Cuts at: 0 164 390 421 1038
Size: 164 226 31 617
Fragments arranged by size:
617 226 164 31
BspHI T'CATG_A
Cuts at: 0 500 689 1038
Size: 500 189 349
Fragments arranged by size:
500 349 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 70 1038
Size: 70 968
BsrGI T'GTAC_A
Cuts at: 0 2 197 869 1038
Size: 2 195 672 169
Fragments arranged by size:
672 195 169 2
BstEII G'GTnAC_C
Cuts at: 0 82 1038
Size: 82 956
Bsu36I CC'TnA_GG
Cuts at: 0 970 1038
Size: 970 68
DraIII CAC_nnn'GTG
Cuts at: 0 428 465 1038
Size: 428 37 573
Fragments arranged by size:
573 428 37
EaeI y'GGCC_r
Cuts at: 0 467 1038
Size: 467 571
EarI CTCTTCn'nnn_
Cuts at: 0 768 1038
Size: 768 270
EcoICRI GAG'CTC
Cuts at: 0 162 1038
Size: 162 876
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 1038
Size: 148 890
EcoRI G'AATT_C
Cuts at: 0 127 1038
Size: 127 911
EcoRV GAT'ATC
Cuts at: 0 669 1038
Size: 669 369
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 822 1038
Size: 822 216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 349 381 1002 1034 1038
Size: 349 32 621 32 4
Fragments arranged by size:
621 349 32 32 4
MscI TGG'CCA
Cuts at: 0 469 1038
Size: 469 569
MslI CAynn'nnrTG
Cuts at: 0 199 499 679 694 1038
Size: 199 300 180 15 344
Fragments arranged by size:
344 300 199 180 15
PflMI CCAn_nnn'nTGG
Cuts at: 0 465 1038
Size: 465 573
PfoI T'CCnGG_A
Cuts at: 0 538 1038
Size: 538 500
PstI C_TGCA'G
Cuts at: 0 395 1038
Size: 395 643
SacI G_AGCT'C
Cuts at: 0 164 1038
Size: 164 874
SfcI C'TryA_G
Cuts at: 0 391 963 1038
Size: 391 572 75
Fragments arranged by size:
572 391 75
SspI AAT'ATT
Cuts at: 0 705 1038
Size: 705 333
StyI C'CwwG_G
Cuts at: 0 408 1038
Size: 408 630
TatI w'GTAC_w
Cuts at: 0 2 197 869 1038
Size: 2 195 672 169
Fragments arranged by size:
672 195 169 2
XmnI GAAnn'nnTTC
Cuts at: 0 131 805 1038
Size: 131 674 233
Fragments arranged by size:
674 233 131
Enzymes that do cut:
AarI AlfI ApoI AseI BanII BbvCI BclI BpmI
Bpu10I BseRI BseYI BsiHKAI Bsp1286I BspHI BspMI BsrGI
BstEII Bsu36I DraIII EaeI EarI EcoICRI Eco57MI EcoRI
EcoRV FalI Hin4I MscI MslI PflMI PfoI PstI
SacI SfcI SspI StyI TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EagI EciI EcoNI EcoO109I FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI ZraI